Eb Biotek Rep I S2
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Transcript of Eb Biotek Rep I S2
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PERKEMBANGANTEKNOLOGI REPRODUKSI
Mas Yedi SumaryadiEDISI 1
Program PascasarjanaUniversitas Jenderal Soedirman, Purwokerto
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MATERI KULIAH
PERKEMBANGAN TEKNOLOGI REPRODUKSI PADA TERNAK:
TEKNIK MANIPULASI BERAHI TEKNIK INSEMINASI BUATAN TEKNIK SUPEROVULASI TEKNIK TRANSFER EMBRIO TEKNIK MIKROMANIPULASI EMBRIO, TRANSGENIK DAN
SEX SEPARASI TEKNIK PENGAWETAN MATERIAL GENETIK (BANK
GENETIKA) PELUANG RISET PENGEMBANGAN TEKNOLOGI
REPRODUKSI
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IB ET
PERKEMBANGAN TEKNOLOGI
REPRODUKSI
INEFISIENSI GAMET KASUS KLINIK
TRANSPLANTASI
SEMEN BEKU EMBRIO BEKU
MANIPULASI BERAHISUPEROVULASIIVF – IVM – IVEP
RG
MANIPULASI EMBRIO
TRANSGENIK
PRESERVASI
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PROFIL HORMON P/E SELAMA SIKLUS BERAHI
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PRECURSOR HORMON
KAJI TERAP PADA TERNAK RUMINANSIA TINGKAT KEBERHASILAN 90 – 100 % UJI COBA KERBAU PEMALANG
UMUM HORMONAL
APLIKASI APLIKASI TEKNOLOGITEKNOLOGIMANIPULASI MANIPULASI BERAHIBERAHI
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TEKNIK MANIPULASI BERAHI SECARA HORMONAL
BerahiPGF 2 IMPGF 1 IM
BerahiExert CIDR/IVS
Insert CIDR/IVS
H12-14H11Ho
H12-14H11Ho
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TEKNIK MANIPULASI BERAHI DENGAN PRECURSOR HORMON
NaOH 2N
CaCl2 jenuh
FRAKSINASILIMBAH CAIR
MINYAK
SABUN KALSIUM ASAM LEMAK
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PRODUKSI SEMEN BEKU DAN TEKNIK IB
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10 %
30 %
Sentrifugasi
Atas Sperma XBawah Sperma Y
Buang supernatannya
SEX SEPARASI GRADIEN PUTIH TELUR
Susilawati et al., 1997
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TEKNOLOGI SEX SEPARASITEKNOLOGI SEX SEPARASI
• ADANYA KECENDERUNGAN PANGSA PASAR SAPI POTONG MENGHENDAKI ANAK JANTAN UNTUK FEEDLOTER, SEDANGKAN SAPI PERAH ANAK BETINA
• ANAK HASIL IB BISA DISESUAIKAN DENGAN KEINGINAN
• PEMISAHAN DILAKUKAN SEBELUM PENGENCERAN DAN PEMBEKUAN
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TEKNOLOGI SEX SEPARASITEKNOLOGI SEX SEPARASI
• PENENTUAN JENIS KELAMIN DITENTUKAN KROMOSOM SEX
• SAPI MEMILIKI 60 KROMOSOM SEX• 29 PASANG AUTOSOM DAN 1
PASANG GENOSOM (KROMOSOM SEX)
• KROMOSOM SEX BETINA ( X DAN X)• KROMOSOM SEX JANTAN (X DAN Y)• GAMET (OVUM DAN SPERMATOZOA)
ADALAH SEL HAPLOID YANG MENGANDUNG KROMOSOM X ATAU Y
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SEX SEPARASI
SPERMA YANG MENGANDUNG KROMOSOM X DISEBUT SPERMATOZOA X
SPERMA YANG MENGANDUNG KROMOSOM Y DISEBUT SPERMATOZOA Y
KOLKISIN DAPAT MENAHAN PEMBELAHAN KROMOSOM SEX PADA FASE METAFASE
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PERBEDAAN SIFAT SPERMA X DAN Y
SPERMA Y SPERMA XKARAKTERISTIK
LEBIH SEDIKITLEBIH BANYAK 3-4%
DNA
LEBIH KECILLEBIH BESARUKURAN TUBUH
PANCAR SINAR
TDK BERSINARIDENTITAS
LEBIH CEPATLEBIH LAMBATGERAKAN
ANODAKATODAMUATAN
ASAMBASADERAJAT pH
ACIDOPHOBIC LEBIH PENDEK
ACIDOPHOBIC LEBIH LAMA
KEMOTAKSIS
LEBIH CEPATLEBIH TAHANDAYA HIDUP
BULATOVALBENTUK KEPALA
LEBIH KECILLEBIH BESARUKURAN KEPALA
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IDENTIFIKASI
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TEKNIK PEMISAHAN SEDIMENTASI (70 % BETINA DARI SPERMA X
MENGENDAP DAN LAPISAN ATAS SPERMA Y JANTAN)
ELEKTROFORESIS: 70, 1% SPERMA Y TERTARIK KE ARAH ANODA (POSITIF) DAN 87,8% SPERMA X TERTARIK KEARAH KATODA
IMUNOLOGIS: SPERMA Y BANYAK HY ANTIGEN
TEKNIK DENSITAS DAN VISCOSITAS DENGAN BOVINE SERUM ALBUMEN, PUTIH TELUR, PERCOLL: SPERMA Y PERGERAKAN CEPAT DARI PADA X
TEKNIK FILTRASI DENGAN SEPHADEX GEL FILTRASI DENGAN PENGURAN PANJANG DAN LEBAR KEPALA SPERMA
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TEKNIK SEDIMENTASI
• WAKTU SENTRIFUGASI
• RPM
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TEKNIK ELEKTROFORESIS
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TEKNIK IMUNOLOGIS
HY-ANTIGEN
Spo Sp1
ANTIBODI
PRODUKSI ANTIBODI
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APLIKASI DI LAPANGAN
ANTIBODI
ANTIGEN ANAK 1
ANAK 2
ANAK 3
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TEKNIK DENSITAS PERCOLL (ALBUMEN)
Semen GradienSegar putih telur 10 dan 30%
Pencucian PBS DulbeccosSentrifugasi 1500 rpm 5 menitSupernatan dibuang3. Filtrasi sephadex G-200
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TEKNIK FILTRASI SEPHADEX
Kolom Gel Sephadex
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TEKNIK SUPEROVULASI
H12-14H11H9Ho
IBPGF2FSH-LH
PGF 1
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Program Donor dengan Multiple OvulasiHo H11 H14 H25 H27 H29-H30 H36 PGF1
PGF2 BerahiPMSG-HCGPGF3IB 3 x
KoleksiEmbrioProgram ResipienHoH11H15H26H27H29-H30H36--PGF 1PGF 2-BerahiTransferEmbrioEN
TEKNIK DONOR RESEPI