Curriculum vitae

Dr. İkbal Agah İNCE

PERSONAL DATA

Address : Acıbadem University

Kerem Aydınlar Campus

School of Medicine

Department of Medical Microbiology

İçerenköy Mah. Kayışdağı Cad. No: 32

Ataşehir-İstanbul

Tel : 0090-216-5004124

0090-505-5268494

Fax : 0090-216-5765120

e-mail : agah.ince@acibadem.edu.tr

ikbal.agah.ince@gmail.com

Websites : Google Scholar

: LinkedIn

EXPERIENCE

Associate Professor at Acıbadem University

September 2013 - Present

Fundamental Research Lines; - Detection of pathogen signals in complex biological samples using well established model organisms (in Human, Drosophila, Tsetse).

- Manipulation of the host (vector) microbiota to block transmission of pathogens.

Innovative Research Lines; - Developing diagnostic tools for infection diseases especially Tuberculosis diagnosis on site.

- Exploring novel bioactive compounds in use for biotechnological and industrial purposes isolated from microbial sources including antibiotics,

immunosuppressive agents, anti-tumor agents.

- Applying non-targeted integrated meta approaches for characterization of marine sediments and unexplored microcosms.

Administrative roles; - Responsibility on Establishing Research and Development Projects and International Cooperation

Joint Research Associate at Laboratory of Biochemistry - Wageningen University

April 2012 - Present Generation of microbe-host protein interaction networks for developing mitigation strategies for infectious diseases.

Visiting Scientist at Laboratory of Virology - Wageningen University

April 2006 - October 2012 (PhD in Virology/Biochemistry)

Functional genomics of Chilo iridescent virus: a transcriptoproteomic approach. PhD Thesis dissertation

Research Assistant at Karadeniz Technical University March 2004 - April 2006 (PhD in Microbiology)

- Discovery of a novel inhibitor of apoptosis protein (IAP) in iridoviruses

- Construction of transient expression systems for testing anti apoptotic effects of viral proteins in cell culture models.

- Application of RNA interferens approaches for functional analysis of viral genes in cell culture

- Supervision of MSc students

Research Assistant at Ondokuz Mayıs University

September 2000 - April 2004 (MSc in Biochemistry)

-Experience in research animal model system (BALB/C mice) in basic research and nonclinical drug discovery.

-Hybridoma development and monoclonal antibody production

EDUCATION

PhD degree*

Functional genomics of Chilo iridescent virus: A transcriptoproteomic approach (2012)

Joint venture of the Laboratories of Virology and Biochemistry, Wageningen University, the Netherlands

*One of the publications from the dissertation selected as best PhD publication of the Graduate School - The thesis rated with Upper second class degree by the academic board.

PhD degree**

Functional analysis of a putative inhibitor genes of apoptosis (iap) encoded by an iridovirus model (2007)

Turkish Scientific and Technological Research Council, TUBİTAK

Institute of Natural and Applied Science, Karadeniz Technical University, Turkey

**Selected as the best PhD thesis from the Institute of Natural and Applied Science in the year 2007

–The thesis rated with First Class Degree.

Master of Science

Effect of nicotine and melatonin in liver antioxidant enzyme status in Swiss albino laboratory mice (2003)

Experience in research animal model systems (BALB/C mice) in basic research and nonclinical drug discovery

Hybridoma development and monoclonal antibody production

Institute of Natural and Applied Sciences, Ondokuz Mayıs University, Turkey

-First Class Degree; GPA 4.00/4.00

AWARDS

- Microbiology Society Grant, UK, 2016

- Agreenskills Scientist Mobility Grant, EU-FP7 2013

- FEMS Congress Grant for Young Scientists, UK, 2013

- Society for General Microbiology, UK, 2010

- Society for Invertebrate Pathology, USA, 2009

- *Best PhD Publication Award, Graduate School for PE&RC, NL, 2009

- Society for General Microbiology, UK, 2008

- FEMS Young Scientist Meeting Grant, UK, 2008

- **Postgraduate Encouragement Award, Karadeniz Technical University, TR, 2008

- Society for Invertebrate Pathology, USA, 2007

- Personal Research Grant, Laboratory of Virology, WUR, NL, 2007

- Doctoral Research Fellowship, TUBITAK, 2005

RESEARCH INTERESTS & SPECIALISATION*

Molecular Virology and Biochemistry – Microbe-host interaction, Microbial Pathogenesis Applied molecular virology approaches with DNA and RNA viruses; Functional genomics, Transcriptomics,

Cell/virus culture, Quantitative proteomics, Protein-Protein interactions, in silico identification of protein interaction

networks, Structural proteomics, Production of recombinant viruses, Gateway technology, Bac-to-Bac System, Protein

expression in eukaryotic and prokaryotic systems in vivo or in vitro, yeast two-hybrid system, pull down assays,

Split--luciferase assay; Reverse genetic applications, Immuno-labelling, Electron, & Fluorescent Microscopy

Current activity focus; Characterization of microbe-host protein interaction networks to unravel pivotal RNA and

proteins for developing mitigation or intervention strategies to control microbe infections.

*Extensive technical experience in molecular biology, virology and biochemistry

PROFFESIONAL MEMBERSHIP

Society of Invertebrate Pathology – USA

Society for General Microbiology – United Kingdom

Federation of European Microbiological Society, United Kingdom

Nederlandse Vereniging voor Microbiologie, the Netherlands

ASM- The American Society for Microbiology, USA

SELECTED PUBLICATIONS (h index=10)

1. Henry Muriuki Kariithi , İkbal Agah İnce, Sjef Boeren, Edwin Kimathi Murungi, Irene Kasindi Meki, Everlyne Achieng' Otieno, Steven Reuben Ger Nyanjom, Monique M. van Oers, Just M. Vlak and Adly M. M. Abd-Alla. Comparative analysis of salivary gland proteomes of two Glossina species with differential hytrosavirus pathologies. Frontiers Microbiology – Virology, 7:89, 2016 | http://dx.doi.org/10.3389/fmicb.2016.00089

2. Adly M.M. Abd-Alla, Henry M. Kariithi, François Cousserans, Nicolas J. Parker, İkbal Agah İnce, Erin D. Scully, Sjef Boeren, Scott M. Geib, Solomon Mekonnen, Just M. Vlak, Andrew G. Parker, Marc J. B. Vreysen, Max Bergoin. Comprehensive Annotation of the Glossina pallidipes Salivary Gland Hypertrophy Virus from Ethiopian Tsetse Flies: A proteogenomics Approach. Journal of General Virology, 2016 | http://dx.doi.org/10.1099/jgv.0.000409

3. İkbal Agah İnce, Sjef Boeren, Monique M. van Oers, Just M. Vlak. Temporal proteomic analysis and label-free quantization of viral proteins of an invertebrate iridovirus. Journal of General Virology, 96: 196-205, 2015

4. İkbal Agah İnce, Zihni Demirbağ and Hatice Katı. Arthrobacter pityocampae sp. nov., isolated from Thaumetopoea pityocampa (Lep., Thaumetopoeidae). International Journal of Systematic and Evolutionary Microbiology, 64: 3384-3389, 2014

5. Amir Mirzadi Gohari, Rahim Mehrabi, Olivier Robert, İkbal Agah İnce, Sjef Boeren, Martin Schuster, Gero Steinberg, Pierre JGM Wit, Gert HJ Kema. Molecular characterization and functional analyses of ZtWor1, a transcriptional regulator of the fungal wheat pathogen Zymoseptoria tritici. Molecular Plant Pathology, 15: 394-405, 2014

6. Henry M. Kariithi, Jan van Lent, İkbal Agah İnce, et al. Correlation between structure, protein composition, morphogenesis and cytopathology of GpSGHV. Journal of General Virology, 94:193-208, 2013

7. Arvind Kumar, İkbal Agah İnce, Ahmet Katı and Ranadhir Chakraborty. Brevibacterium siliguriense sp. nov., a novel facultatively oligotrophic bacterium isolated from river water. International Journal of Systematic and Evolutionary Microbiology, 63, 511–515, 2013

8. İkbal Agah İnce, Kadriye Özcan, Monique van Oers, Just M. Vlak. Temporal classification and mapping of nonpolyadenylated transcripts of an invertebrate iridovirus. Journal of General Virology, 94: 187-92, 2013

9. Alfred W. Bronkhorst, Koen W. R. van Cleef, Nicolas Vodovar, İkbal Agah İnce, Hervé Blanc, Just M. Vlak, Maria-Carla Saleh and Ronald P. van Rij. The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery. PNAS, 109: 3604–3613, 2012

10. Yeşim Aktürk Dizman, Zihni Demirbağ, İkbal Agah İnce et al. Transcriptomic analysis of Chilo iridescent virus immediate early promoter. Virus Research, 167: 353-357, 2012

11. Henry M. Kariithi, İkbal Agah İnce, Sjef Boeren, Adly Abd-Alla, Andrew G. Parker, Serap Aksoy, Just M. Vlak and Monique van Oers. The salivary secretome of the tsetse fly Glossina pallidipes (Diptera: Glossinidae) infected by salivary gland hypertrophy virus. PloS Neglected Tropical Diseases, 5: e1371. 2011

12. Fang Xu, İkbal Agah İnce, Sjef Boeren, Monique van Oers, Just M. Vlak. Protein composition of the occlusion derived virus of Chrysodeixis chalcites nucleopolyhedrovirus. Virus Research, 158: 1-7, 2011

13. Henry M. Kariithi, İkbal Agah İnce, Sjef Boeren, Jacques Vervoort, Max Bergoin, Monique van Oers, Addly M.M. Abd-Alla, Just M. Vlak. Proteomic analysis of Glossina pallidipes Salivary Gland Hypertrophy Virus virions for immune intervention in tsetse fly colonies. Journal of General Virology, 91: 3065–3074, 2010

14. İkbal Agah İnce, Sjef Boeren, Monique M. van Oers, Jacques J.M. Vervoort, Just M. Vlak. Proteomic analysis of Chilo iridescent virus particles. Virology, 405: 253-258, 2010

15. Hatice Katı, İkbal Agah İnce, İsmail Demir, Zihni Demirbağ. A novel bacterium, Brevibacterium pityocampa sp. nov., isolated from Thaumetopoea pityocampa Den. and Schiff., (Lep., Thaumetopoeidae). International Journal of Systematic and Evolutionary Microbiology, 60: 312-316, 2010

16. Hatice Katı, İkbal Agah İnce, Kazım Sezen, Zihni Demirbağ Characterization of two Bacillus thuringiensis subsp. morrisoni strains isolated from Thaumetopoea pityocampa Den. and Schiff., (Lep., Thaumetopoeidae). Biocontrol Science & Technology, 19: 475-484, 2009

17. Agata K. Jakubowska, İkbal Agah İnce, Salvador Herrero, Just M. Vlak, Monique M. van Oers. Spodoptera exigua nucleopolyhedrovirus is not infectious for Agrotis segetum larvae per os, but only after intrahemocoelic injection, IOBC/WPRS Bulletin 45: 99-102 2009.

18. İkbal Agah İnce, Zafer Eren, İrem Gürkanlı. Effect of melatonin on the antioxidant enzyme status in the liver of Swiss albino laboratory mice in single circadian rhythm. Journal of Applied Biological Sciences, 3: 1-6, 2009.

19. İkbal Agah İnce, Marcel Westenberg, Just M. Vlak, Zihni Demirbağ, Remziye Nalçacıoğlu, Monique M. van Oers. Open reading frame 193R of Chilo iridescent virus encodes a functional inhibitor of apoptosis (IAP), Virology, 376: 124-131, 2008

20. İkbal Agah İnce, Hatice Katı, Hüseyin Yılmaz, İsmail Demir, Zihni Demirbağ. Isolation and identification of entomopathogenic bacteria from Thaumetopoea pityocampa Den. and Schiff., (Lep., Thaumetopoeidae) and determination of their biocontrol potential. World Journal of Microbiology and Biotechnology, 24: 3005-3015, 2008

21. Remziye Nalçacıoğlu, İkbal Agah İnce, Just M. Vlak, Zihni Demirbağ and Monique M. van Oers. The Chilo iridescent virus DNA polymerase promoter contains an essential AAAAT motif. Journal of General Virology, 88: 2488-2494, 2007

22. İkbal Agah İnce, İsmail Demir, Zihni Demirbağ and Remziye Nalçacıoğlu. A cytoplasmic polyhedrosis virus isolated from the pine processionary caterpillar, Thaumetopoea pityocampa, Journal of Microbiology and Biotechnology, 17: 632-637, 2007

PRESENTATIONS (International symposia, workshops and conferences) (>20)

Regularly attended (several as a invited speaker) international congress, meetings or symposiums since 2006

PROJECT SUPERVISIONS

- PHIBI: Uncovering Pathogen-Host Interactions of Fungal Infections - BioInformatic services and multi-level

modeling of molecular networks to identify biomarkers and drug targets for infectious diseases".

- Investigation of Viral Infection Mechanisms through Bioinformatic Analysis of Virus-Human Protein-Protein

Interaction Networks Using Systems Biology Approach

- Factors associated with killing speed of ascovirus infection

- Identification of Dengue virus-encoded inhibitors of programmed cell death in insect cells

- Development of a pentavalent dengue virus vaccine

- Determination of the intermolecular disulfide bond of Helicoverpa armigera SNPV F protein

- Analysis of the Invertebrate iridovirus 6 DNA polymerase gene promoter region

- Functional analysis of Invertebrate iridovirus 6 anti-apoptotic proteins

- The role of apoptosis in cell to cell dissemination of Invertebrate iridovirus 6 infection

- Determination and functional analysis of structural proteins of Invertebrate iridovirus 6

- Proteomic analysis of GpSGHV for immune intervention in tsetse fly colonies

- The salivary gland sialome of the tsetse fly (G. pallidipes) infected by salivary gland hypertrophy virus

- Determination of protein-protein interactions of IIV-6 virion proteins using yeast two hybrid system

- Determination of protein composition of two life forms (ODV and BV) of Chrysodeixis chalcites SNPV

- Secretome study on plant pathogenic fungi mycosphaerella

- Construction of recombinant bacmid producing a hairpin construct against S. exigua protein kinase gene

- Screening marine microorganisms for novel enzyme activities by innovative functional metagenomic approaches

- Expression of iridoviral kinases by heterologous virus gene expression system using Gateway technology

- Characterization of novel components of the baculovirus per os infectivity factor complex (PIF)

VOLUNTEER EXPERIENCE

Student Representative at Society of Invertebrate Pathology

August 2007 - September 2011 (4 years 2 months) Organizing educational activities for postgraduate students.

SGM Society Champions Program

August 2014 - (present) Arranging talks within their workplace/learning environment on the Society's behalf. Promoting membership of the Society to their immediate

colleagues, students, networks. Arranging for the display of Society promotional materials within their workplace/at events.