COMMUNAL LIVING
Transcript of COMMUNAL LIVING
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can be made up of a multitude of bacterial species, some of which may have never been studied in a lab. They make for a for-midable adversary.
But researchers are starting to accu-mulate enough knowledge about how bio-films form to think seriously about how to control them. “In the past 10 or 15 years, we’ve seen the techniques and approaches of modern biology and genetic and mo-lecular tools applied to biofilms,” says Phil Stewart, director of the Center for Biofilm Engineering (CBE) at Montana State University. “That has helped change the biofilm from an amorphous primitive slab of slime into a biologically sophisticated,
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This clean room will be used to generate and study biofilms as part of the BASF Advanced Research Initiative at Harvard University.
through which nutrients are distributed. In the end, they are better off living as a colony than going it alone.
That safety in numbers poses a chal-lenge for anyone trying to get rid of a biofilm, which can plant itself virtually any-where. Bacteria are tricky enough to deal with on their own; they’ve spent billions of years learning to overcome whatever envi-ronmental threats come their way.
So imagine the power of bacteria banded together, living under something akin to a force field, and acting in unison in ways scientists have only recently begun to un-derstand. In addition to being able to thrive in pretty much any environment, biofilms
IT WAS LONG ASSUMED that bacteria were loners that floated through their single-celled existence without need of companionship. That simple, independent lifestyle made it pretty easy to study them in a test tube or grow them in a petri dish.
But it turns out that bacteria are actu-ally social creatures. They congregate and chemically communicate, working together to stay alive. More often than not, they exist in complex communities called biofilms, started up when one cell sticks to a surface in an aqueous environ-ment and somehow signals others to join in. Those recruits, along with descendents of that first cell, create a protective matrix
COVER STORY
Imagine the power of bacteria banded together, living under
something akin to a force field, and acting in unison.
COMMUNAL LIVINGScientists across academia and industry are making a concerted effort
to understand and control BACTERIA THAT FORM BIOFILMS
LISA M. JARVIS, C&EN NORTHEAST NEWS BUREAU
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BIOFILM TOPOGRAPHY Weibel’s lab uses
interferometry to create two-dimensional
arrays of Pseudomonas biofilms.
differentiated, and regulated community.”Indeed, academic researchers are in-
trigued and industry executives seem ready to devote money to real-world methods of manipulating biofilms. Everyone involved believes it is going to take a coordinated effort across the biological and physical sciences to better understand and harness these bacterial communities.
Biofilms’ ubiquity makes them a ripe subject for both academia and industry. If you have ever slipped on a rock while walking in a stream, you’ve encountered a biofilm. Run your tongue across the surface of your teeth; that plaque buildup you feel is actually a biofilm. When you’re standing over your sink wondering why the water refuses to drain, blame a biofilm.
But biofilms can have a more costly im-
pact. Bacteria like to freeload on the hulls of ships. Biofouling, or the accumula-tion of bacteria and other material on an underwater surface, exacts hefty fuel costs on the shipping industry. Antifouling paints can delay the process, but eventually bacteria will glom on. And removing the films takes a toll on the environment; those bacterial communities adhere to the sur-face so strongly that it takes chlorine and heavy-duty scraping to remove them.
Meanwhile, biofilms also have a major impact on human health. Hospital-ac-quired infections are now the fourth-lead-ing cause of death in the U.S. behind heart disease, cancer, and stroke. The culprit behind most of those infections? Biofilms. Bacteria can thrive for months on hospital surfaces—floors, instruments, gloves—and be passed on to open wounds or to implanted devices such as catheters, heart valves, and artificial hips where biofilms can form.
YET BIOFILMS can also be useful. Indus-trial wastewater treatment centers have long exploited bacteria’s propensity to suck in anything that looks tasty. Biofilms are incorporated into water treatment sys-tems to filter out organic compounds and pathogens.
Despite the prevalence of biofilms, the discovery that bacteria like to form their own gated communities was discounted for years. Most scientists finally took no-tice of biofilms in the mid-1990s. Since then, the number of journal articles about the topic has risen exponentially, CBE’s Stewart says.
Several underlying developments are spurring interest in biofilms. For one, ideas about bacteria are changing. “It used to be that we thought of microorganisms as being these little things that were all roughly the same clonal descendents of some maternal line who talked to one another, if at all, dis-tantly,” says George M. Whitesides, profes-sor of chemistry at Harvard University and surface science expert.
Now, scientists are beginning to under-stand that bacteria have a complex commu-nication system, the properties of which are dictated, at least in part, by biofilms. In a film, there’s no circulation or blood flow,
no con-vective mass transport, Whitesides notes. “Concentrations of a chemical can build up to higher levels; there’s an efficiency of chemical commu-nication that occurs in a biofilm that would not occur otherwise.”
And importantly, more people—be they scientists or members of the public—understand that “biofilms actually mat-ter,” Stewart says. “When you go out into nature, or in engineered systems, and increasingly in the medical context, the microorganisms really are banded together in aggregate.”
The human health threat, in particular, has attracted attention to the problem. Al-though ships have long dragged and pipes have forever clogged, those problems never seemed urgent, notes Roberto Kolter, pro-fessor of microbiology and molecular ge-netics at Harvard Medical School. But the proliferation of implants and other medical devices means more and more pernicious infections caused by the biofilms that love to call the devices home. Antibiotics are perplexingly ineffective against bacteria contained within a biofilm.
Once a few geneticists started point-ing out that bacteria like to spend most of their time on surfaces, “interest really ex-ploded,” Kolter says. Advanced microscopy and other scientific tools have aided both in convincing people that bacteria like the communal lifestyle and in facilitating re-searchers’ ability to study and understand how biofilms form.
One sure sign that biofilms are ripe for exploration is industry involvement. After all, the ultimate goal is learning to control the films in the real world. Last fall, BASF formed the BASF Advanced Research Ini-tiative at Harvard University, a partnership that brings together researchers from both organizations to tackle scientific chal-lenges. BASF put up $20 million to fund the
COVER STORYB
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five-year program, and biofilms are the first project on tap.
“Biofilms on surfaces cause multi-billion-dollar
losses each year,” Franz Brandstetter, head of BASF’s
Polymer Research Competence Center, told reporters at a sym-
posium on biofilms held in April to mark the launch of the partner-
ship. Montana State’s CBE also works closely with industry on biofilms. It has
32 member companies and ran projects aimed at solving biofilm-related problems
for 43 companies last year.Because biofilms are magnificently com-
plex systems, it will take a concerted effort by academia and industry to unravel the details of their formation and function.
SCIENTISTS ARE also weighing a chicken-or-egg question: Should they first develop a better understanding of biofilm forma-tion and makeup, or is it possible to head straight to products and methods to con-trol bacteria and then work backward to glean knowledge about how the systems work?
CBE’s Stewart wants to charge ahead. “If we try to wait until we have every gene in the circuit figured out, we’re not going to get there,” he says. “We need to do some fundamental science and build the infor-mation base, but this is the right time to begin actually exploring technologies.”
Biofilms’ complexity is daunting, but it also lends itself to myriad methods of attack. Scientists can imagine many ways to prevent or control a biofilm. One route is to create surfaces that don’t let bacteria stick at all. There are also multiple oppor-tunities for intercession during the forma-tion of a biofilm or after it has matured; researchers are considering everything from how the film feeds itself to how its composition changes over time. Scientists are even considering how they might en-courage a benign biofilm to grow on a sur-face, thereby preventing a more pernicious one from attaching itself.
Many scientists are working to find the overarching principles to which, despite their diversity, all biofilms subscribe. Find-ing those commonalities could accelerate the search for broad means of control. Thus, the hunt is on for general themes, such as how surface properties affect bio-film formation and how a change in the en-vironment affects the destiny of a colony.
The varying perspectives of the chem-
ists, biologists, medical doctors, and physi-cists involved in the field means a wide range of scientific approaches. Harvard’s Kolter, for example, is trying to understand the cellular changes that occur during biofilm formation and how different cell types organize themselves within the film matrix. By monitoring the gene expression of three important cell types—those re-
sponsible for motility, matrix production, and sporulation within a Bacillus subtilisbiofilm—scientists in Kolter’s lab found that the population of each type changed over time and was distributed based on the architecture of the colony.
Kolter also uncovered evidence of a developmental pathway by which the mo-tile cells transition to matrix production
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18WWW.CEN-ONLINE.ORG JUNE 9, 2008
and then to sporulation. Furthermore, he found checkpoints during biofilm forma-tion when interrupting the development of a cell will change its fate. “If you arrest normal development by having a mutation that does not make the matrix, the bacte-ria don’t progress to the next stage; they somehow get stuck,” Kolter says.
These checkpoints are something scien-tists previously wouldn’t have associated with bacterial communities, Kolter says, and could eventually provide the means to control or mediate them.
Whitesides’ lab, meanwhile, is bring-
ing its expertise in shaping and defining the mechanical properties of gels—a good proxy for slimy biofilms—to help elucidate the interface between a biofilm and a sur-face. The goal is to learn which properties of the biofilm are molecular and which are mechanical. “We don’t know whether the characteristics of the biofilm are intimately connected to the properties of the mol-ecules that are there or primarily a result of the gel and its influence on mass trans-port,” Whitesides says.
Biofilm formation occurs in two stages, Whitesides points out. The first is when a
bacterial cell has to stick to a surface—be it steel, Teflon, or bone—and form a gel-like matrix. The second is when subsequent parts of the biofilm stick to that matrix. Whitesides is interested in building gels to understand those two interfaces—one be-tween a surface and a microbial gel and one between two gels.
RESEARCHERS IN Whitesides’ lab are taking several tacks to explore those inter-actions. For example, they are using self-assembled monolayers (SAMs) to build structured molecular interfaces that have
COVER STORY
A lot of time and effort is
spent trying to rid the world
of biofilms, those slicks of
bacteria that cling to the
inside of pipes, the bottom
of ships, and the surface of
medical devices. But what if
the sticky, stubborn nature
of biofilms could be put to
good use? Biofilms have
long been applied as natural
filters in industrial wastewa-
ter treatment systems, and
researchers are now contem-
plating other ways they could
be exploited.
“We know biofilms can
be very stable, effective
systems. In fact, a lot of
conventional wastewater
treatment systems use them,
and they’re very robust and
efficient,” says Phil Stewart,
director of the Center for
Biofilm Engineering at Mon-
tana State University.
BASF, the world’s largest
chemical company, is at-
tempting to exploit biofilms
to make key compounds via
biocatalytic means. A biofilm
can be seen as nothing more
than a thin-film catalyst, says
Bernhard Hauer, the firm’s
vice president of biocatalysis
research. Hauer’s group is
trying to make viable reac-
tors in which a chemical
substrate is pumped over a
support coated with a biofilm
that converts the substrate
into a product. Scientists
at BASF hope to replace
conventional fermentation
processes and suspension-
based cultures with reactors
containing biofilms.
The process could offer
several advantages over
conventional fermentation.
A biofilm is relatively stable,
whereas batch fermentation
requires starting or inoculat-
ing a reactor every other day,
Hauer notes. Biofilms can
offer high cell density, Stew-
art adds, resulting in a lot of
reactivity in a small volume.
Because of the fixed nature
of biofilms, another advan-
tage is that the solution
coming out the other end of
the reactor is relatively free
of biomass. Moreover, the
biofilm can be immediately
reused as a catalyst.
So far, Hauer’s group has
constructed reactors filled
with rings made of several
materials—metals, glass, and
ceramics—as a support on
which the biofilms grow. After
experimenting with more
than 70 strains of bacteria,
the scientists found that
some thrived on specific sup-
ports, while others planted
themselves on whatever was
around. The fact that the
films grow so easily on a wide
variety of materials also cre-
ates complications; BASF has
to use special filters on either
end of the reactor to keep the
bacteria from growing back-
wards into the feedstock.
In the end, Hauer says,
steel rings proved to be a
suitable support for these
early systems, although BASF
will revisit the support ques-
tion if the systems are ex-
panded to commercial scale.
The trials also showed that
the biofilm systems are sta-
ble for more than 100 days.
Once a system was worked
out, Hauer’s group first at-
tempted catalytic conver-
sion by trying to create
R-2-butanol from a racemic
mixture of 2-butanol. The
group was able to isolate
R-2-butanol using a biofilm
but had trouble working at
high concentrations. Still,
the experiment proved the
system was viable, and the
researchers moved on to use
a biofilm to oxidize ethylene
glycol to glycoxylic acid.
Other groups have tried this
oxidation using Escherichia
coli without success. BASF
found a Pseudomonas-based
biofilm that proved to be
highly effective at turning
out the desired product.
Currently, BASF scientists
are working to increase the
concentration of starting
material while maintaining a
stable system. They are also
trying to identify the genes
that metabolize glycoxylic
acid and then use proteom-
ics to block that process and
improve the yield to 90%.
“Biofilms look promising,”
Hauer says. “Most industrial
strains form them, they are
metabolically active for a long
time, there isn’t fouling in
the reactor, and the specific
investment seems to be lower
than batch fermentation.”
BA
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EXPLOITING BIOFILMS
BASF Explores Using Bacterial Surfaces To Its Advantage
Hauer
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20WWW.CEN-ONLINE.ORG JUNE 9, 2008
both adsorbing and nonadsorbing surfaces and are then studying how well compounds attach to them. One goal is to understand how biofilm formation correlates with hydrophilicity, hydrophobicity, and other surface properties.
By screening for nonfouling surfaces, his group has been successful in finding a handful of SAMs that resist protein ad-sorption. Whitesides is also trying to build three-dimensional microfluidic devices—systems of micrometer-scale channels and wells that mimic the chemistry and physics of biological systems. Those 3-D approaches could help scientists build lay-ered biofilm systems that contain different organisms and closely replicate what is go-ing on in nature.
Rustem F. Ismagilov, a chemist at the University of Chicago, is using microfluid-ics to understand biofilms’ spatial struc-ture, which dictates the flow of nutrients, environmental signals, and how cells with-in the film chemically talk to each other. The structure also influences metabolic interactions within films, including the breakdown of molecules.
“We can’t understand biofilms until we understand the spatial structure,” Is-magilov says. This means determining the main bacterial players, how those players function depending on their location, and how that function changes as the biofilm matures and the external environment varies.
THE GOAL is to better understand how the highly organized structure enables cells within biofilms to act in concert. Such an un-derstanding could provide new ideas about how to break the structure down or recreate it in synthetic systems that mimic some of the functions performed by biofilms.
Douglas B. Weibel, a biochemist at the University of Wisconsin, Madison, is studying the point in biofilm development when that first bacterial cell adheres to a surface. His lab is trying to understand how a cell senses it is on a surface—a seemingly basic step that is well understood in mam-malian cells but is a mystery in bacterial cells.
Weibel first wants to figure out the en-vironmental trigger that tells the cell it is in contact with a surface and next to deter-
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mine the transcrip-tional change that tells it to abandon its solitary lifestyle. Although scientists have yet to prove whether that change is chemical or physi-
cal, Weibel, like Whitesides, is looking at a host of physical phenomena including sur-face stiffness, surface tension, hydropho-bicity, hydrophilicity, viscosity, and osmo-lality. The idea is that, despite differences between bacteria, commonalities based on physical phenomena could provide starting points for controlling bacterial behavior.
Others are diving directly into real-world problems by tapping general knowl-edge about bacteria and human biology and then extrapolating to how biofilms distrib-ute nutrients. For example, microbiology professors E. Peter Greenberg and Pradeep Singh, both at the University of Washing-ton, Seattle, are exploiting basic informa-tion about bacteria’s dependence on iron, which is required for biofilm development, to develop drugs that treat biofilm-related infections.
Singh found that lactoferrin, an iron-binding antimicrobial factor present in hu-man tears, sweat, mucus, and other bodily
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SURFACE STUDY
Ye Jin (Jenna) Eun, a graduate student in Weibel’s lab, works on a biofilm experiment.
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Scientists need to recognize the enormous
diversity contained in a biofilm.
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21WWW.CEN-ONLINE.ORG JUNE 9, 2008
fluids, sends a signal to bacteria that iron in the area is low, prompting them to move on rather than squat and form a biofilm. Ehud Banin, of Israel’s Bar Ilan University, and Greenberg showed that normal bio-film development needs a threshold level of cellular iron. Putting those concepts together led to the idea that the efficacy of antibiotics, which are notoriously bad at killing bacteria within a biofilm, could be increased by somehow limiting iron in the cell.
Singh decided to take a Trojan horse strategy and gain access to the cell using the ironlike element gallium, which inter-feres with bacterial iron metabolism. Banin and Greenberg combined efforts with Mottie Chevion, a chemist at Hebrew Uni-versity of Jerusalem, to make gallium-con-taining compounds that look like a source of iron to bacteria. In the lab, several com-pounds seem to enhance the efficacy of an-tibiotics, and early experiments in animals are validating the gallium approach.
IN ADDITION to potentially yielding new drugs, the research by Greenberg and Singh could explain why antibiotics are unable to kill bacteria living in a biofilm. They have shown that the combination of a gallium-containing compound with the antibiotic gentamicin is more effective against bio-films than either treatment on its own. Greenberg hypothesizes that the antibiotic is killing the bacteria on the surface of the biofilm, which is well fed and therefore susceptible to treatment, while the gallium compound is killing the bacteria in the middle of the biofilm, which is starved and scavenging for iron or ironlike compounds.
Despite the wide range of approaches to unraveling the complexity of biofilms and inventing ways of controlling them, there are some basic housekeeping issues that need to be resolved before any product can make it to market.
Wisconsin’s Weibel points to the issue of reproducibility. At present, the tech-niques for generating biofilms in the lab are not very precise, to the detriment of me-thodical study. For example, researchers would like to be able to make a whole slew of identical biofilms and then study each one under different conditions to decide which conditions are important and which are not.
“This is one area where I think chemists can have a big impact,” Weibel says. “We know how to control surface chemistry, which means we know how to control
where cells get seeded on a surface. We can take those concepts and extend them into techniques to make reproducible biofilms.”
Being able to generate reproducible films could also ease the registration of antibiofilm products, mainly disinfectants, with the Environmental Protection Agen-cy. “The test EPA uses to register disin-fectants is from the 1950s,” CBE’s Stewart
says. CBE is working with EPA, the Food & Drug Administration, and a number of companies to develop standardized meth-ods for testing biofilms.
The lack of agreed-upon standards for making biofilms also extends into the medical arena. The first drug to combat a biofilm “is going to be a hard one,” Uni-versity of Washington’s Greenberg says.
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COVER STORY
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“Whoever does this is going to have to work closely with FDA.”
Meanwhile, the Harvard and BASF re-searchers have ambitious goals for their biofilms collaboration. Andreas Kreimeyer, executive director for research at BASF, figures it will take about three years to parse through the general understanding of biofilms to decide which product opportu-nities are most viable. The approaches for tackling biofilms are vast, and the scientists at BASF and Harvard are likely to first con-sider how to develop controlled surfaces, adds David Weitz, a physicist at Harvard.
REAL-LIFE SOLUTIONS—new medical materials or ship coatings that can resist biofilms—are still years away. While work in the lab has largely focused on simple systems combining one surface and one strain of bacteria, the real world is never so simple. Most biofilm systems comprise multiple species of bacteria, including many organisms that have never been char-acterized, Kolter cautions. It’s a big leap to get from studying the basic mechanisms of a single cell to understanding what’s hap-pening on a ship’s hull, he says.
Scientists need to recognize the enor-mous diversity contained in a biofilm and consider that there may not be a single solution or even a set of them, Kolter says. Still, researchers are optimistic that a con-certed push across many disciplines will accelerate efforts to control biofilms. “This is an area where we think the problem is both complicated enough to be interest-ing, but simple enough that well-defined approaches will lead to good results,” Har-vard’s Whitesides says. ■
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