Cellometer X2 Image Cytometer Cellometer Reagents ......Cellometer Reagents Catalog # Description...

・（ビール）醸造酵母・ワイン酵母・血小板その他小さい細胞

Cellometer X2 Image CytometerOptimized Analysis for Yeast and other Small Cells

Features of the Cellometer X2

Dual Fluorescence and Bright Field Imaging: staining of both live and dead cells in yeast samples

User-Friendly Software and Assay Selection: Enhanced inter-operator reproducibility, minimal training, auto-save option

Fast Results: Obtain cell images, counts, size measurements, and viability calculations in 60 seconds

Small Sample Size: Only 20 µl of sample

Broad Dynamic Range: Measurable concentration range of 2.5 x 105 to 5 x 107 cells/mL using Nexcelom’s proprietary de-clustering function

Many Compatible Dyes: Trypan blue, AO, PI, EB, 7AAD, AO/PI, AO/EB, Calcein AM, CFDA-AM, Calcein AM/PI, CFDA/PI

Learn why thousands of users, including the top ten pharmaceutical companies, trust Cellometer.

On-Line Demonstrations are completed in just 20to 30 minutes and provide an overview of howCellometer works using existing images of cells that interest you.

On-Site Demonstrations are a convenient way to test a Cellometer system for a specific application. An experienced Applications Specialist will arrive at your lab for a hands-on session to test your cells and show how Cellometer can enhance your workflow.

Technical Seminars are an excellent way tointroduce Cellometer systems to a lab group or collaborators in different laboratories within anorganization. A trained biologist will discuss and demonstrate the capabilities and advantages of Cellometer image cytometry.

Call 978-327-5340 or E-mail [email protected] today to schedule a free demonstration or technical seminar.

Advantages of Cellometer Image Cytometer

Cell Imaging• Verify cell morphology and counted live/dead cells

• Export cell images for presentations and publications

Pattern Recognition Software• Accurately count cells in clumps

• Count irregular-shaped cells

• Eliminate debris from cell counts

• Differentiate cells based on size

Automated Data Management• Pre-set assays and automated reports

• Archive sample images and auto-save results

Maintenance-free System• Disposable counting chambers – no wash steps

• No required instrument maintenance

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See www.nexcelom.com/products for updated product selections.

For more information, visitwww.nexcelom.com

Contact us at:Nexcelom Bioscience360 Merrimack Street, Building 9Lawrence, MA 01843, USA

Email: [email protected]: 978.327.5340Fax: 978.327.5341

Which Cellometer is Right for Me?

Features Automated Cell Counters Image Cytometers

Mini Auto T4

Auto 1000

Auto 2000

X4 (10x) X1 X2 K2 Vision

CBAVision CBA (10x)

Cell / Sample Type

Objective Magnification 4X 4X 4X 4X 10X 10X 10X 4X 5X 10X

Cell Line X X X X X X

Cultured Primary Cells X X X X X X

Algae X X

Platelets X X X

Low Cocnentration Cell Lines X X X

Yeast (Clean Sample) X X X

Primary cells (Messy Sample*) X X X

PBMCs, Splenocytes, Stem Cells X X X

Yeast (Messy Sample) X X

Hepatocytes X X

Adipocytes*** X X X

Cell-Based Assay ** X X X X X

Apoptosis (Annexin V-FITC/PI) X X X

Apoptosis (Caspase Activity) X X X

Autophagy (CytoID-green) X X

Cell Proliferation (CFSE) X X

Cell Cycle (PI) X X X X X

GFP Transfection X X X X X

YFP Transfection X X

RFP Transfection X X

Mitochondrial Potential (JC-1) X X

Multi-drug Resistance (ABC Transporter) X X

Surface Marker Analysis X X

Vitality (Calcein-AM/PI) X X X X

Image Cytometry** X X* A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.** FCS Express 4 license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter

Cellometer Cell Counters, Cell Analysis Systems & Image Cytometry Nexcelom offers a wide range of Cellometer systems developed and optimized for specific applications and cell types.

Simply Counted Image Cytometer

Cellometer Reagents

Catalog # Description Instrument Compatibility Size Unit

CS1-0108-5ML AO (acridine orange) Staining Solution for staining of nucleated cells Auto 2000, K2, X2, X1, X4, Vision CBA 5 mL each

CS1-0109-5ML PI (propidium iodide) Staining Solution for staining of dead nucleated cells Auto 2000, K2, X2, X4, Vision CBA 5 mL each

CS2-0106-5ML AO/PI (acridine orange / propidium iodide) Staining Solution for live/dead Mammalian nucleated cells Auto 2000, K2, X2, Vision CBA 5 mL each

CS1-0114CS0-0115-100ML

CS1-0116Cellometer Annexin V-FITC / PI Apoptosis Reagents X2, K2, Vision CBA each

K183-100-NK183-25-N

Cellometer Caspase-3 Apoptosis Kit X2, K2, Vision CBA each

K188-100-NK188-25-N

Cellometer Caspase-8 Apoptosis Kit X2, K2,Vision CBA each

CSK-0112 Cellometer PI Cell Cycle Kit X1, X2, K2, Vision CBA each

CSK-0102 Cellometer ViaStain Kit for live/dead Yeast concentration including stainer buffer, fluorescent dye mixture X2, K2, Vision CBA

Cellometer Counting Chamber

Catalog # Description Size Unit

CHT4-PD100-003 Standard chamber thickness. Packed in microscope slide boxes. Ready to use.Case of 500 slides for 1,000 counts (10 individual boxes)

1 Case

CHT4-SD100-014Standard chamber thickness. Packed with protective film on both sides. Remove protective film before use.

Case of 900 slides for 1,800 counts 1 Case

CHT4-PD300-003 3x standard chamber thickness. Packed in microscope slide boxes. Ready to use.Case of 500 slides for 1,000 counts (10 individual boxes)

1 Case

イメージサイトメーター細胞カウンティング＆解析

Cellometer

® X2

Brewing YeastWine YeastPlateletsand Other Small Cells

セロメーター X2 イメージサイトメーター酵母や小さい細胞の解析に最適です。

セロメーター X2の特徴蛍光2色と明視野でのイメージング：酵母サンプルの生死判定が可能です。

簡便なソフトウェアと解析：再現性の強化、トレーニング不要、自動データ保存

迅速な測定：細胞イメージの取得、カウント、サイズ計測、生存率算出まで60秒以内

サンプル容量：わずか20μl

広範囲な検出可能濃度領域：ネクセローム社独自のデクラスター機能により、 2.5 x 105 - 5 x 107 cells/mLが検出可能

様々な色素に対応：トリパンブルー、AO、PI、EB、７AAD、AO/PI、AO/EB、Calcein AM、Calsein AM/PI、CFAD/PI

トップ製薬企業をはじめとするユーザーがセロメーターを信頼する理由をデモンストレーションで体感してください。

デモンストレーションビデオ

下記URLにて、セロメーターの使い方や測定原理の概要について知ることができます。１ビデオは数分で見ることができます。　※英語ビデオhttp://www.nexcelom.com/Support/Cellometer-Video-Library.html

オンサイトデモンストレーション

アプリケーションスペシャリストが訪問し、実際の細胞サンプルを用いての測定をハンズオンでお見せします。セロメーターが細胞カウント作業をいかに簡単で正確なものにするかを体感することができます。

ご用命はトミーデジタルバイオロジー株式会社へ電話：03－5834－0810E-mail:[email protected]

セロメーターの利点

細胞イメージング• 細胞の形状や生死判定の確認• 画像をエクスポートし、プレゼン資料や論文へ使用可能

細胞認識ソフトウェア• 凝集した細胞も分離して識別• 通常と異なる形状もカウント可能• 夾雑物をカウント対象から除外• サイズの違いによる識別

自動データ管理• 測定メニューと自動レポートを標準装備• サンプル画像と自動データ保存

メンテナンスフリー• 使い捨てのカウンティングチャンバーで洗浄不要• 装置本体のメンテナンス不要

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See www.nexcelom.com/products for updated product selections.

For more information, visitwww.nexcelom.com

Contact us at:Nexcelom Bioscience360 Merrimack Street, Building 9Lawrence, MA 01843, USA

Email: [email protected]: 978.327.5340Fax: 978.327.5341

Which Cellometer is Right for Me?

Features Automated Cell Counters Image Cytometers

Mini Auto T4

Auto 1000

Auto 2000

X4 (10x) X1 X2 K2 Vision

CBAVision CBA (10x)

Cell / Sample Type

Objective Magnification 4X 4X 4X 4X 10X 10X 10X 4X 5X 10X

Cell Line X X X X X X

Cultured Primary Cells X X X X X X

Algae X X

Platelets X X X

Low Cocnentration Cell Lines X X X

Yeast (Clean Sample) X X X

Primary cells (Messy Sample*) X X X

PBMCs, Splenocytes, Stem Cells X X X

Yeast (Messy Sample) X X

Hepatocytes X X

Adipocytes*** X X X

Cell-Based Assay ** X X X X X

Apoptosis (Annexin V-FITC/PI) X X X

Apoptosis (Caspase Activity) X X X

Autophagy (CytoID-green) X X

Cell Proliferation (CFSE) X X

Cell Cycle (PI) X X X X X

GFP Transfection X X X X X

YFP Transfection X X

RFP Transfection X X

Mitochondrial Potential (JC-1) X X

Multi-drug Resistance (ABC Transporter) X X

Surface Marker Analysis X X

Vitality (Calcein-AM/PI) X X X X

Image Cytometry** X X* A messy sample is a heterogeneous sample containing unwanted cell types, such as red blood cells, in addition to the cells of interest.** FCS Express 4 license must be purchased in order to perform Cell Based Assay or Image Cytometry analysis*** Cellometer CHT4-PD300 slides are required for cells greater than 80µm in diameter

Cellometer Cell Counters, Cell Analysis Systems & Image Cytometry Nexcelom offers a wide range of Cellometer systems developed and optimized for specific applications and cell types.

Simply Counted Image Cytometer

Cellometer Reagents

Catalog # Description Instrument Compatibility Size Unit

CS1-0108-5ML AO (acridine orange) Staining Solution for staining of nucleated cells Auto 2000, K2, X2, X1, X4, Vision CBA 5 mL each

CS1-0109-5ML PI (propidium iodide) Staining Solution for staining of dead nucleated cells Auto 2000, K2, X2, X4, Vision CBA 5 mL each

CS2-0106-5ML AO/PI (acridine orange / propidium iodide) Staining Solution for live/dead Mammalian nucleated cells Auto 2000, K2, X2, Vision CBA 5 mL each

CS1-0114CS0-0115-100ML

CS1-0116Cellometer Annexin V-FITC / PI Apoptosis Reagents X2, K2, Vision CBA each

K183-100-NK183-25-N

Cellometer Caspase-3 Apoptosis Kit X2, K2, Vision CBA each

K188-100-NK188-25-N

Cellometer Caspase-8 Apoptosis Kit X2, K2,Vision CBA each

CSK-0112 Cellometer PI Cell Cycle Kit X1, X2, K2, Vision CBA each

CSK-0102 Cellometer ViaStain Kit for live/dead Yeast concentration including stainer buffer, fluorescent dye mixture X2, K2, Vision CBA

Cellometer Counting Chamber

Catalog # Description Size Unit

CHT4-PD100-003 Standard chamber thickness. Packed in microscope slide boxes. Ready to use.Case of 500 slides for 1,000 counts (10 individual boxes)

1 Case

CHT4-SD100-014Standard chamber thickness. Packed with protective film on both sides. Remove protective film before use.

Case of 900 slides for 1,800 counts 1 Case

CHT4-PD300-003 3x standard chamber thickness. Packed in microscope slide boxes. Ready to use.Case of 500 slides for 1,000 counts (10 individual boxes)

1 Case

Image Cytometer for Cell Counting & Analysis

Cellometer

® X2

醸造工場で用いられている酵母一般的に、醸造工場で使用されている酵母は非常にクリーンです。これらの酵母はセロメーターX1およびX2カウント可能です。細胞濃度や生存率は明視野および蛍光測定によって算出することが可能です。

酵母の解析

Performance of the Cellometer X2 Image Cytometer

Concentration Dynamic Range Figure 3 depicts the dynamic range for cell concentration measured by Cellometer X1. This data set was taken on a concentration series of cultured yeasts.

Samples from 4 x 105 – 5 x 107 cells/ml can be counted without further dilution.

The %CV at each concentration was below 10%.

Consistency and Repeatability The results indicate the accuracy of the Cellometer X1 instrument in assessing the viability of yeasts using PI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X1 in measuring cell concentration and viability of yeast cells.

Cellometer X2 Image Cytometer for Yeast & Other Small Cells

from Nexcelom Bioscience

How It Worksé

Pipette 20 µl of Cell Sample

Insert Counting Chamber

Select Assay & Click Count

Get Results

Yeast Viability Analysis by DualFluorescence

Small Chain Yeast Culture Analysis

Platelet Analysis

Small Cell Concentration and Viability Analysis

Yeast Vitality Analysis

Sperm Analysis

Yeast Cell Cycle Analysis

ASSAYS

é

Cellometer Performance

Yeast AOPI ViabilitySmall Chain Yeast CulturePlateletsWindsor Ale RehydratedSmall Cell ConcentrationWine Yeast Rehydrated PI ViabilityYeast Vitality CFDA AMSpermYeast Cell Cycle

Cellometer®

SET UPAssayYeast AOPI Viability

Dual FL/BR Channels

Easily Edit and Import Assays

Images for Data Verification

Cell Size Histograms

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シングルセルカウントデクラスターカウント鎖状セルカウント

明視野での酵母の濃度測定

明視野と蛍光による酵母の濃度＆生存率測定

Propidium Iodide (PI)による生存率測定明視野で全細胞数を、蛍光で死細胞をカウントし、生存率を算出します。

Oxonolによる生存率測定明視野で全細胞数を、蛍光で死細胞をカウントし、生存率を算出します。

X1 X2

明視野イメージングモード ○ ○

1色蛍光イメージングモード ○ ○

2色蛍光イメージングモード ○

PIによる生存率 ○ ○

AOPIを用いた細胞濃度および生存率 ○

CFDA-AMを用いた生育能 ○

酵母細胞周期 ○ ○

デクラスター認識および鎖状細胞の識別 ○ ○

平均直径およびサイズ分布表示 ○ ○

新規細胞パラメーターの作成 ○ ○

Cell Cycle Analysis Using Propidium Iodide (PI)を用いた細胞周期解析一般的なパン酵母をNexcelom Bioscience社のCell Cycle Staining Kit で染色し60分インキュベート後、セロメーターX2で細胞周期を解析を行いました。左図はPI蛍光強度をヒストグラムで示しています。DNA量から活動期のポピュレーションが示されます。

酵母の細胞周期解析

酵母の生存率と生育能

2色蛍光による生存率測定酵母サンプルとアクリジンオレンジ（AO）・プロピジウムアイオダイド（PI)混合液とを1：1で混合し蛍光染色します。染色後すぐにセロメータX2で測定し、酵母の細胞濃度と生存率を算出します。生細胞は緑色蛍光、死細胞は赤色蛍光で検出されます。

酵素蛍光染色による生存率測定V

酵母サンプルとCarboxyfluorescein-AM を１：１で混合し酵素蛍光染色した後、セロメーターX2で生存率を算出します。明視野で全細胞を蛍光で活性酵母細胞を検出します。

全血中の血小板自動カウント

蛍光による結小板濃度の測定

血液サンプルをNexcelom Bioscience社のCalcein AM Vitality / Viability Kit で20分インキュベートして染色します。血小板と白血球が緑色蛍光を発するので、サイズで識別し血小板のみをカウントします。


Samples from 2.5 x 105 – 5 x 107 cells/ml can be counted without further dilution.


y = 1.0059xR2 = 0.99961

0.00E+001.00E+072.00E+073.00E+074.00E+075.00E+076.00E+077.00E+078.00E+07

0.00E+00 2.00E+07 4.00E+07 6.00E+07 8.00E+07

Conc

entr

atio

n (C

ells

/ml)

Expected Value (Cells/ml)


Cellometer X1 Average Total Cell Concentration via Bright-Field

Viability

AVE 4.07E+07 69.0%STDEV 2.28E+06 2.5%CV(%) 5.61 3.64

y = 1.026x

0.0E+00 2.0E+07 4.0E+075.0E+07 7.0E+07Expected Value (Cells/ml)

0.0E+001.0E+072.0E+07

3.0E+07

4.0E+07

5.0E+076.0E+07

7.0E+07

Conc

entr

atio

n (C

ells

/ml)

1.0E+07 3.0E+07 6.0E+07

R2 = 0.99482

↑

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FEATURES

Viability Dynamic Range The viability dynamic range is 0 - 100% for Cellometer X2 Image Cytometer using dual fluorescence AO/PI stain.

Figure 1. Table of results for cell concentration dynamic range

Cellometer X2 Average Live Cell Concentration Via Fluorescence

Viability

AVE 1.32E+07 78.1%STDEV 7.69E+05 2.2%CV(%) 5.84 2.78

Figure 2: Table of results for cell concentration and viability using acridine orange (AO) and propidium Iodide (PI)

Consistency and Repeatability The results indicate the accuracy of the Cellometer X2 instrument in assessing the viability of yeasts using AOPI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X2 in measuring cell concentration and viability of yeast cells.

Viability Dynamic Range The viability dynamic range is 0 - 100% for Cellometer X1 Image Cytometer using PI stain.


Figure 4. Table of results for cell concentration and viability using bright-field and Propidium iodide (PI)

Yeast Used in Brewing Industry

In general, yeast strains used in the brewing industry are very clean. They are counted using Cellometer X1 and X2 Image Cytometers. Concentration and viability are measured using Cellometer bright field and fluorescent images.

Cellometer Yeast Analysis



Samples from 4 x 105 – 5 x 107 cells/ml can be counted without further dilution.


Consistency and Repeatability The results indicate the accuracy of the Cellometer X1 instrument in assessing the viability of yeasts using PI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X1 in measuring cell concentration and viability of yeast cells.

酵母や小さい細胞用セロメーター　X2

操作方法é

サンプルを20μl充填

スライドチャンバーを本体へ挿入

Assayを選択し、カウントボタンをクリック

結果

２色蛍光で酵母の生死判定

小さい鎖状酵母の培養解析

血小板の解析

小さい細胞の濃度・生死判定

酵母の生育能解析

精子解析

酵母の細胞周期解析

解析

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Cellometer Performance


Cellometer®


2色蛍光/BR解析プログラムを簡単に編集・

インポート画像でデータを検証

細胞サイズをヒストグラム

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Single Cell Count De-clustering of Yeast Cells Chain-Forming Cell Count

Yeast Concentration Measurement by Bright Field Analysis

Yeast Concentration & Viability Measurement by Bright Field & Fluorescence

Viability Measurement Using Propidium Iodide (PI) Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

Viability Measurement by Oxonol Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

X1 X2

Bright field imaging mode X X

Single fluorescence imaging mode X X

Dual fluorescence imaging mode X

Viability using PI X X

Concentration & viability using AOPI X

Vitality using CFDA-AM X

Yeast cell cycle X X

Cellometer software for analysis of clumpy and irregular-shaped cells

X X

Mean diameter and cell size distribution X X

Cell type wizard for creating new cell type parameters X X

Cell Cycle Analysis Using Propidium Iodide (PI) Standard baker’s yeast stained with the Cell Cycle Staining Kit from Nexcelom Bioscience are incubated for 60 minutes before using Cellometer X2 to analyze the cell cycle. The plot shows the yeast population that is actively dividing. Their higher DNA content is measured using PI.


Yeast Viability and Vitality

Viability by Dual-Fluorescence Yeast samples are stained 1-to-1 with a mixture of acridine orange (AO) and propidium iodide (PI) dual-fluorescence stain. Yeast concentration and viability are obtained immediately after staining using Cellometer X2. Live yeast cells fluoresce green and dead cells fluoresce red.

Vitality by Fluorescent Enzymatic Stain Yeast samples are stained 1-to-1 with Carboxyfluorescein-AM fluorescent enzymatic stain for 45 minutes and then analyzed for vitality using Cellometer X2. Bright field images are used for total cell count and fluorescent images are used to measure the active yeast cells.

Automated Platelet Counting in Whole Blood

Fluorescence-Based Platelet Concentration Measurement A blood sample stained using the Calcein AM Vitality / Viability Kit from NexcelomBioscience is incubated for 20 minutes. Both platelets and white blood cells produce green fluorescence. Cell size gating is applied toexclusively count platelets.


Samples from 2.5 x 105 – 5 x 107 cells/ml can be counted without further dilution.


y = 1.0059xR2 = 0.99961

0.00E+00

1.00E+07

2.00E+07

3.00E+07

4.00E+07

5.00E+07

6.00E+07

7.00E+07

8.00E+07

0.00E+00 2.00E+07 4.00E+07 6.00E+07 8.00E+07

Conc

entr

ation

(Cel

ls/m

l)



Cellometer X1 Average Total Cell Concentration via Bright-Field

Viability

AVE 4.07E+07 69.0%

STDEV 2.28E+06 2.5%

CV(%) 5.61 3.64

y = 1.026x

0.0E+00 2.0E+07 4.0E+07 5.0E+07 7.0E+07


0.0E+00

1.0E+07

2.0E+07

3.0E+07

4.0E+07

5.0E+07

6.0E+07

7.0E+07

Conc

entr

ation

(Cel

ls/m

l)

1.0E+07 3.0E+07 6.0E+07

R2 = 0.99482

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機能

Viability Dynamic Range The viability dynamic range is 0 - 100% for Cellometer X2 Image Cytometer using dual fluorescence AO/PI stain.


Cellometer X2 Average Live Cell Concentration Via Fluorescence

Viability

AVE 1.32E+07 78.1%

STDEV 7.69E+05 2.2%

CV(%) 5.84 2.78

Figure 2: Table of results for cell concentration and viability using acridine orange (AO) and propidium Iodide (PI)

Consistency and Repeatability The results indicate the accuracy of the Cellometer X2 instrument in assessing the viability of yeasts using AOPI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X2 in measuring cell concentration and viability of yeast cells.

Viability Dynamic Range The viability dynamic range is 0 - 100% for Cellometer X1 Image Cytometer using PI stain.


Figure 4. Table of results for cell concentration and viability using bright-field and Propidium iodide (PI)

Yeast Used in Brewing Industry

In general, yeast strains used in the brewing industry are very clean. They are counted using Cellometer X1 and X2 Image Cytometers. Concentration and viability are measured using Cellometer bright field and fluorescent images.

Cellometer Yeast Analysis

セロメータX2の性能評価

細胞濃度の測定範囲 Figure 3ではセロメータX1で酵母希釈系列の細胞濃度を測定した時の濃度範囲を示しています。

4 x 105 – 5 x 107 cells/mlの濃度範囲ではサンプルを希釈することなく測定することが可能です。

各濃度の %CVは10％以下でした。

整合性と再現性Figure 4ではセロメーターX1を用い、PIで酵母の細胞濃度と生存率を24回測定した時の正確性を示しています。

Cellometer X2 Image Cytometer for Yeast & Other Small Cellsfrom Nexcelom Bioscience

How It Worksé

Pipette 20 µl of Cell Sample

Insert Counting Chamber

Select Assay & Click Count

Get Results

Yeast Viability Analysis by DualFluorescence

Small Chain Yeast Culture Analysis

Platelet Analysis

Small Cell Concentration and Viability Analysis

Yeast Vitality Analysis

Sperm Analysis


ASSAYS

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セローメーターの性能


Cellometer®


Dual FL/BR Channels

Easily Edit and Import Assays

Images for Data Verification

Cell Size Histograms

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Single Cell Count De-clustering of Yeast Cells Chain-Forming Cell Count

Yeast Concentration Measurement by Bright Field Analysis

Yeast Concentration & Viability Measurement by Bright Field & Fluorescence

Viability Measurement Using Propidium Iodide (PI) Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

Viability Measurement by Oxonol Bright field images are used to obtain total cell count, while fluorescent images are used to count dead cells.

X1 X2

Bright field imaging mode X X

Single fluorescence imaging mode X X

Dual fluorescence imaging mode X

Viability using PI X X

Concentration & viability using AOPI X

Vitality using CFDA-AM X

Yeast cell cycle X X

Cellometer software for analysis of clumpy and irregular-shaped cells

X X

Mean diameter and cell size distribution X X

Cell type wizard for creating new cell type parameters X X

Cell Cycle Analysis Using Propidium Iodide (PI) Standard baker’s yeast stained with the Cell Cycle Staining Kit from Nexcelom Bioscience are incubated for 60 minutes before using Cellometer X2 to analyze the cell cycle. The plot shows the yeast population that is actively dividing. Their higher DNA content is measured using PI.


Yeast Viability and Vitality

Viability by Dual-Fluorescence Yeast samples are stained 1-to-1 with a mixture of acridine orange (AO) and propidium iodide (PI) dual-fluorescence stain. Yeast concentration and viability are obtained immediately after staining using Cellometer X2. Live yeast cells fluoresce green and dead cells fluoresce red.

Vitality by Fluorescent Enzymatic Stain Yeast samples are stained 1-to-1 with Carboxyfluorescein-AM fluorescent enzymatic stain for 45 minutes and then analyzed for vitality using Cellometer X2. Bright field images are used for total cell count and fluorescent images are used to measure the active yeast cells.

Automated Platelet Counting in Whole Blood

Fluorescence-Based Platelet Concentration Measurement A blood sample stained using the Calcein AM Vitality / Viability Kit from NexcelomBioscience is incubated for 20 minutes. Both platelets and white blood cells produce green fluorescence. Cell size gating is applied toexclusively count platelets.

細胞濃度の測定範囲

Figure 1 にセロメーターX2で酵母の希釈系列を測定したグラフを示します。

2.5 x 105 – 5 x 107 cells/ml の範囲では、希釈することなくカウントすることが可能です。各濃度のCV（％）は10％以下でした。

y = 1.0059xR2 = 0.99961

0.00E+00

1.00E+07

2.00E+07

3.00E+07

4.00E+07

5.00E+07

6.00E+07

7.00E+07

8.00E+07

0.00E+00 2.00E+07 4.00E+07 6.00E+07 8.00E+07

Conc

entr

ation

(Cel

ls/m

l)

セロメーター　X1の性能

セロメーター　X1 明視野での細胞濃度測定生存率

AVE 4.07E+07 69.0%STDEV 2.28E+06 2.5%CV(%) 5.61 3.64

y = 1.026x

0.0E+00 2.0E+07 4.0E+07 5.0E+07 7.0E+070.0E+00

1.0E+07

2.0E+07

3.0E+07

4.0E+07

5.0E+07

6.0E+07

7.0E+07

Conc

entr

ation

(Cel

ls/m

l)

1.0E+07 3.0E+07 6.0E+07

R2 = 0.99482

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FEATURES

生死判定の範囲AO/PIを用いた２色蛍光染色によって、セロメーターX2で0 - 100% の範囲で生死判定できます。


Figure 1. 細胞濃度の測定可能範囲

セロメーターX2 蛍光による細胞濃度測定生存率

AVE 1.32E+07 78.1%STDEV 7.69E+05 2.2%CV(%) 5.84 2.78

Figure 2: AO/PI染色による細胞濃度と生存率の測定結果

整合性と再現性AOPIを用いた生死判定の正確性をFigure 2に示します。24回繰り返し測定し、生存率の平均を算出しました。この結果から、酵母の細胞濃度および生存率をセロメーターX2で測定した時の信頼性と再現性が示されました。

生死判定の範囲PIを用いた蛍光染色によって、セロメーターX１で0 - 100% の範囲で生死判定できます。

Expected Value

(Cells/ml) Figure 3. 細胞濃度の測定可能範囲

Figure 4. 明視野とPropidium iodide (PI)による細胞濃度と生存率の測定結果

Cellometer X2 Image Cytometer Cellometer Reagents ......Cellometer Reagents Catalog # Description...

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