CAT201502-Protein Research Product Catalogblossombio.com/pdf/promotion/CAT201502-Protein Research...

8
伯森蛋白質研究產品專刊 伯森蛋白質研究產品專刊 伯森蛋白質研究產品專刊 ProteinResearchProductCatalog ProteinResearchProductCatalog ProteinResearchProductCatalog Expression LEXSY Protein Expression System P. 2 Purification Affinity Size Exclusion Ion Exchange FPLC Accessories P. 3 P. 5 P. 6 P. 6 Crystallization Screening Kit Crystallography Starter Kit Detergents P. 7 P. 7 P. 7 Labeling FluoroSpin Protein Labeling and Purification Kit P. 8 B B 伯森生物科技股份㈲限公司 Blossom Biotechnologies Inc. 網址:www.blossombio.com 客服專線:台北0800-059668台南0800-259988花蓮0800-056668 台北(02)2788-2121 新⽵(037)581-106 台中(04)2323-3939 台南(06)235-6628 ⾼雄(07)555-0764

Transcript of CAT201502-Protein Research Product Catalogblossombio.com/pdf/promotion/CAT201502-Protein Research...

伯森蛋白質研究產品專刊伯森蛋白質研究產品專刊伯森蛋白質研究產品專刊Protein�Research�Product�Catalog�Protein�Research�Product�Catalog�Protein�Research�Product�Catalog�

Expression

LEXSY ProteinExpression System

P. 2

Purification

Affinity

Size Exclusion

Ion Exchange

FPLC Accessories

P. 3

P. 5

P. 6

P. 6

Crystallization

Screening Kit

Crystallography Starter Kit

Detergents

P. 7

P. 7

P. 7

Labeling

FluoroSpin Protein Labeling and Purification Kit

P. 8

BB伯森生物科技股份㈲限公司Blossom Biotechnologies Inc.

網址:www.blossombio.com 客服專線:台北0800-059668��台南0800-259988��花蓮�0800-056668

台北�(02)�2788-2121 新⽵�(037)�581-106 台中�(04)�2323-3939 台南�(06)�235-6628 ⾼雄�(07)�555-0764

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Expression

2

Cultured Leishmania tarentolae cells expressing green uorescent protein.

JB-EGE-1300

JB-EGE-1410

JB-EGE-1420

JB-EGE-2002-15

LEXSYcon2 Expression Kit, for constitutive protein expression

LEXSinduce3 Expression Kit, for inducible expression from integrative constructs

LEXSinduce4 Expression Kit, for inducible expression from episomal constructs

LEXSY in vitro Translation Kit, for plasmid based cell-free protein synthesis

Cat. No. Product Name

LEXSY Protein Expression SystemLEXSY 是德商 Jena Bioscience 的獨家

蛋白質表現系統,以單細胞原蟲為宿

主,兼具了真核生物系統以及大腸桿菌

系統的優點,是您大量表現蛋白的最佳

選擇!

Bacteria Yeaste.g. Pichia

Insect cellse.g. Sf9/21

LEXSY Mammaliancells

LEXSY performs mammalian type of glycosylation (Breitling et al. 2002)

LEXSY strains grow higher densitiesthan insect and mammalian cells

(Fritsche et al. 2007)

LEXSY grows fasterthan insect and mammalian cells

LEXSY�完美克服其他蛋⽩質表現系統常⾒問題

YeastBacteriaInsect &Mammalian cells

§�⽣產蛋⽩質無後修飾§�無法摺疊成正確的構形§�具包涵體

§�後修飾與哺乳⽣物差異⼤§�細胞不易破壞,�抽取蛋⽩質困難

§�表現量低§�⽣⾧速率緩慢§�培養液價格昂貴

LEXSY�特點:

þ��不具包涵體�(inclusion�body)þ��後修飾�(post-translational�modification)�近似哺乳⽣物細胞þ��操作流程條件穩定þ��表現量⾼,產量⼤þ��不具⽣物毒性�(Biosafety�level�1)

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Purification

3

FPLC 純化膠體與管柱伯森公司擁有完整的 FPLC 純化商品,包含親和性層析、膠體過濾、

離子交換層析等,提供來自德國、瑞典與日本的優良產品。各式產品

均擁有極高性價比,歡迎進一步索取相關資料及免費試用服務!

Affinity 親和性層析His-tag 重組蛋白純化

His-tag 重組蛋白偵測

膠體

重⼒流

磁珠

預注管柱

ABT-6BCL-NTANi-10

ABT-6BCL-NTANi-100

ABT-6BCL-NTANi-500

CU-31103

CU-31105

ABT-6BCL-QHNi-C8

ABT-6BCL-QHNi-C5

MF-HIS-3000-2ML

MF-HIS-3000-5ML

BW-45-655-103

BW-45-655-104

BW-45-655-107

BW-45-655-108

ABT-AF6NI-CTG5-2

ABT-AF6NI-CTG5-5

NICKEL NTA Agarose Resin, 10 ml

NICKEL NTA Agarose Resin, 100 ml

NICKEL NTA Agarose Resin, 500ml

PureCube Ni-NTA Agarose, 10 ml

PureCube Ni-NTA Agarose, 50 ml

His-COLUMN HIGH Density NICKEL (8x1 ml)

HisXL-COLUMN HIGH Density NICKEL (5x5 ml)

Qbeads-NTA-Ni (2x1 ml)

Qbeads-NTA-Ni (5x1 ml)

BabyBio Ni-NTA (5x1 ml)

BabyBio Ni-NTA (10x1 ml)

BabyBio Ni-NTA (5x5 ml)

BabyBio Ni-NTA (10x5 ml)

NICKEL Afnity Cartridges 5ml (2x5 ml)

NICKEL Afnity Cartridges 5ml (5x5 ml)

NI Sepharose 6FF

NI Sepharose 6FF

NI Sepharose 6FF

NI Sepharose HP

NI Sepharose HP

His Mag Sepharose

His Mag Sepharose

Histrap HP

Histrap HP

Histrap HP

Histrap HP

Histrap FF

Histrap FF

Type Cat. No. Product Name GE 對應商品

JB-PS110 His Detection Kit

Detection of poly-histidine tagged proteins, ready-to-use solutionUp to 3 x 3 reactions

Cat. No. Product Name

His Detection Kit

l 快速偵測 His Tag 重組蛋白純化結果

l 不需 Blocking, Washing 等步驟

l 偵測極限可達 0.1 pmole

l 肉眼即可觀察,也可再使用儀器定量

1 hrTransfer

1-15 hrBlock

30'Wash

1-2 hr1st Ab

30'Wash

1-2 hr2nd Ab

30'Wash

5'Develop

1 hrFix Gel

TOTAL

6-21 hr

20'Wash

1 hrStain

20'Wash

TOTAL

3 hr

1 hrTransfer

30'-90'Incubation

In-GelStain

HisDetection Kit

WesternBlot

>5X�Faster�Than�Western�Blot!TOTAL

1.5-2.5 hr

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Purification

4

GST-tag 重組蛋白純化

抗體純化

其它親和性層析純化

膠體

磁珠

預注管柱

Tag�切除

ABT-4B-GLU-10

ABT-4B-GLU-100

CU-32103

CU-32105

CU-32205

CU-32303

CU-32305

CU-32307

BML-SE363-1000

Glutathione Agarose Resin, 10 ml

Glutathione Agarose Resin, 100ml

PureCube Glutathione Agarose, 10 ml

PureCube Glutathione Agarose, 50 ml

PureCube Glutathione MagBeads (5 ml)

PureCube Glutathione Cartridge (5x1 ml)

PureCube Glutathione Cartridge (1x5 ml)

PureCube Glutathione Cartridge (5x5 ml)

Thrombin

Type Cat. No. Product Name

Glutathione Sepharose 4B

Glutathione Sepharose 4B

Glutathione Sepharose HP

Glutathione Sepharose HP

GSTrap HP

GSTrap HP

GSTrap HP

GE 對應商品

膠體

磁珠

預注管柱

BB-02-01PA-01

BB-02-01PG-01

ABT-4BCLPL-R5

MF-PRA-3000-2ML

MF-PRA-3000-5ML

MF-PRA-3000-10ML

MF-PRG-3000-2ML

MF-PRG-3000-5ML

MF-PRG-3000-10ML

BW-45-605-102

BW-45-605-103

BW-45-605-106

BW-45-605-107

Protein A agarose, 10 ml

Protein G agarose, 10 ml

Protein L agarose, 5ml

Qbeads-Protein A, 2ml

Qbeads-Protein A, 5ml

Qbeads-Protein A, 10ml

Qbeads-Protein G, 2ml

Qbeads-Protein G, 5ml

Qbeads-Protein G, 10ml

BabyBio proteinA (5x1 ml)

BabyBio proteinA (10x1 ml)

BabyBio proteinA (5x5 ml)

BabyBio proteinA (10x5 ml)

Type Cat. No. Product Name

nProtein A Sepharose

Protein G Sepharose

Protein L sepharose

Protein A Mag Sepharose Xtra 5

Protein A Mag Sepharose Xtra 5

Protein A Mag Sepharose Xtra 5

Protein G Mag Sepharose Xtra 5

Protein G Mag Sepharose Xtra 5

Protein G Mag Sepharose Xtra 5

Hitrap protein A HP

Hitrap protein A HP

Hitrap protein A HP

Hitrap protein A HP

GE 對應商品

膠體

磁珠

ABT-6RRF-STV-5

CHI-676943324

CU-50303

CU-33102

MF-STA-3000-5ML

MF-STA-3000-10ML

MF-DEX-3000-5ML

MF-COO-3000-5ML

MF-NHH-3000-5ML

Streptavidin Agarose, 5ml

Cellune sulfate

PureCube NHS Activated Agarose, 10ml

PureCube Rho1D4 Agarose, 5ml

Qbeads-Streptavidin

Qbeads-Streptavidin

Qbeads-Hydroxyl

Qbeads-Carboxyl

Qbeads-Amine

Type Cat. No. Product Name

Streptavidin Sepharose

Heparin Sepharose

NHS Sepharose

Streptavidin Mag Sepharose

Streptavidin Mag Sepharose

GE 對應商品

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Purification

5

Size Exclusion 膠體過濾去鹽與置換緩衝液

Gel Filtration 膠體過濾

離⼼

膠體

重⼒流

CP-0201-Z025.0-001

CP-0201-Z100.0-001

CP-0205-Z025.0-001

CP-0205-Z100.0-001

CP-0202-Z025.0-001

CP-0202-Z100.0-001

TM-0102-E100.0-001

TM-0102-E500.0-001

TM-0105-E100.0-001

TM-0105-E500.0-001

TM-0106-E100.0-001

CP-0109-Z050.0-001

CP-0104-Z050.0-001

CP-0105-Z025.0-001

Z-50 MINI Spin Columns, 25pk

Z-50 MINI Spin Columns, 100pk

Z-25 MINI Spin Columns, 25pk

Z-25 MINI Spin Columns, 100pk

SEQ Z-50 MINI Spin Columns, 25pk

SEQ Z-50 MINI Spin Columns, 100pk

Zetadex-25 Fine, 100g

Zetadex-25 Fine, 500g

Zetadex-50 Fine, 100g

Zetadex-50 Fine, 500g

Zetadex-50, 100g

N2 Gravity Columns (1-200 µl), 50pk

N10 Gravity Columns (0.5-1 ml), 50pk

N25 Gravity Columns (1-2.5 ml), 25pk

Type Cat. No. Product Name

Microspin G-50 columns

Microspin G-50 columns

SpinTrap G-25

SpinTrap G-25

AUTOSEQ G-50

AUTOSEQ G-50

Sephadex G-25 ne

Sephadex G-25 ne

Sephadex G-50 ne

Sephadex G-50 ne

Sephadex G-50 DNA Grade

MiniTrap G-25

PD-10 Desalting Columns

PD-10 Desalting Columns

GE 對應商品

膠體 EMP-TM-0501-E100.0-001

EMP-TM-0501-E500.0-001

ABT-A-1020S-1000

ABT-A-1040S-1000

ABT-A-1060S-1000

ABT-A-1021S-1000

ABT-A-1041S-1000

ABT-A-1061S-1000

Zetadex 20-LH, 100g

Zetadex 20-LH, 500g

2% B Agarose Bead

4% B Agarose Bead

6% B Agarose Bead

2% BCL Agarose Bead

4% BCL Agarose Bead

6% BCL Agarose Bead

Type Cat. No. Product Name

Sephadex LH-20

Sephadex LH-20

Sepharose 2B

Sepharose 4B

Sepharose 6B

Sepharose CL-2B

Sepharose CL-4B

Sepharose CL-6B

GE 對應商品

BabyBio Ni-NTA Mini Columns Z-25 and Z-50 MINI Spin Columns N2, N10, and N25 Gravity Columns

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Purification

6

Ion Exchange 離子交換

膠體

預注管柱

CHI-675982327

CHI-2120051

CHI-675980327

CHI-675983327

CHI-1990751

CHI-2120051

CHI-1980551

CHI-1980051

BW-45-100-107

BW-45-200-103

BW-45-200-107

BW-45-150-103

BW-45-150-107

Cellune Q, 100ml

Cellune S, 100ml

Cellune A, 100ml

Cellune C, 100ml

Cellune Q (5x1 ml)

Cellune S (5x1 ml)

Cellune A (5x1 ml)

Cellune C (5x1 ml)

BabyBio Q (5x5 ml)

BabyBio S (5x1 ml)

BabyBio S (5x5 ml)

BabyBio DEAE (5x1 ml)

BabyBio DEAE (5x5 ml)

Type Cat. No. Product Name

Q Sepharose

SP Sepharose

DEAE Sepharose

CM Sepharose

Hitrap Q FF

Hitrap SP FF

Hitrap DEAE FF

Hitrap CM FF

Hitrap Q HP

Hitrap S HP

Hitrap S HP

Hitrap DEAE HP

Hitrap DEAE HP

GE 對應商品

FPLC Accessories 純化配件

空管柱

磁座

C-50

CXL-50

MC-25

MC-100

ABT-FPLC8-3

ABT-FPLC30-2

ABT-FPLC45-2

ABT-FPLC80-1

O-MAGRACK

PLASTIC columns, 12ml

PLASTIC XL columns, 35ml

PLASTIC MINI columns, 25pk

PLASTIC MINI columns, 100pk

EMPTY FPLC 8ml Columns, 3pk

EMPTY FPLC 30ml Columns, 2pk

EMPTY FPLC 45ml Columns, 2pk

EMPTY FPLC 80ml Columns, 1pk

Mag Rack (1.5 ml x 8 well)

Type Cat. No. Product Name

COLUMN PD-10,EMPTY

Microspin columns

Microspin columns

GE 對應商品

Cellune Chromatography Media PLASTIC Columns (12ml & 35ml) Mag Rack

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Crystallization

7

Screening Kit蛋白質結晶條件多如繁星,JBScreen 系列商品幫助實驗者快速篩選出最適宜的結晶條件!

提供 240 種最常見的緩衝液與沉澱劑之組合

整理出學者研究最容易成功的 96 種條件, 廣泛的 pH 範圍、多種沉澱劑

由 JCSG 機構發表,以稀疏矩陣為原理的篩選組合

針對 Protein Kinase 的初期結晶所設計,涵蓋了 96 種常見結晶條件

對於 PEG 3350 與 PEG 5000 MME 與 48 種鹽類組合的條件篩選

JBScreen Classic

JBScreen PEG/Salt

JBScreen Basic

JBScreen JCSG++

JBScreen Kinase

請前往下列網址瀏覽完整產品項目,或使用 QR code 以獲得進一步資訊

www.jenabioscience.com/cms/en/1/browse/632_crystal_screens.html

™Crystallography Starter Kit

Detergents

l 包含一系列實用工具,讓使用者以省時省力的方式獲取結晶

l MicroMounts™ 具獨家光圈設計,減少取結晶時多餘液體

l 相容於常見 X 光設備,並具極低 X 光背景值

l 堅固耐用,即使在低溫條件下也不變形

JB-CSK-2 Crystallography Starter Kit, with everything to mount and collect X-ray data from your crystals

Cat. No. Product Name

Each kit contains 20 Dual-Thickness MicroMounts™ (M2-L18SP-A2), with 5 each of 75, 100, 150 and 200 µm apertures.

CU-16003

CU-16007

CU-16011

CU-16015

CU-16019

CU-16043

CU-16023

CU-16031

CU-16039

CU-16092

CU-16093

Octylglucopyranoside (OG) (5x5 g)

N,N-Dimethyl-n-dodecylamine N-oxide (LDAO) (5x5 g)

n-Decyl-beta-maltoside (DM) (5x5 g)

n-Dodecyl-beta-maltoside (DDM) (5x5 g)

n-Octyl-1-thio-beta-D-glucopyranoside (OTG) (5x5 g)

n-Decylphosphocholine (Fos-Choline™ 10) (5x5 g)

n-Dodecyl-phosphocholine (Fos-Choline™ 12) (5x5 g)

n-Tetradecylphosphocholine (Fos-Choline™ 14) (5x5 g)

n-Hexadecylphosphocholine (Fos-Choline™ 16) (5x5 g)

Detergent Screening Set Classic (1 g each of OG, LDAO, DM, DDM, OTG)

Detergent Screening Set Phosphocholines 1 (1 g each of Fos-12, Fos-14, and Fos-16)

Features Cat. No. Product Name

純度 >99.5%

專供結晶學使用

for�Sample�Retrieval

伯森生物 / www.blossombio.com / 0800-059668 / (02) 2788-2121

Labeling

8

FluoroSpin Protein Labeling and Purification Kit

FluoroSpin 能將廣泛的螢光物質與大於 25 kDa

的抗體、酵素與其它蛋白質快速地接合與純化。

其特點如下:

l 快速 – 標定加上純化,10分鐘就完成

l 簡單 – 只要三步驟:Mix, Incubate, Purify

l 精確 – 可精確控制蛋白質標定的程度

l 方便 – 產品內已包含 Spin Columns

市⾯上最快速的�Protein�Labeling�Kit�!�

10�分鐘內完成標定�!