Biological Sequence Analysis Project Presentation - SNU · PDF fileBiological Sequence...
Transcript of Biological Sequence Analysis Project Presentation - SNU · PDF fileBiological Sequence...
Biological Sequence Analysis Biological Sequence Analysis Project PresentationProject Presentation
Project2)
Using HMMER to Search Homologues Protein Family
2002-20631
Tutorial Execution to Understand HMMER
Install Linux -> Not Complete
Trial to Dos version Dowonload from HMMER Homepagehttp://hmmer.wustl.edu/
After downloads, creat folder at C:hmmer and file is unzipped.
Get multiple sequence alignment of a protein domain(protein sequence family) from Pfam databaes
http://pfam.wustl.edu/
http://pfam.wustl.edu/http://pfam.wustl.edu/
My interesting protein family is Aminoacyl-tRNA Synthetase(ARS)
What is Aminoacyl-tRNA Synthetase?
ARS domain search from Pfam database
HMM construction with hmmbuild
HMM calibration with hmmcalibrate
Biological Sequence Analysis Project Presentation