BIOL 123 PRACTICAL Complement Mediated Lysis 2014 Data
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Tube No % lysis 1 0.159 0.019 0.272 2 0.293 0.153 2.192 3 0.455 0.315 4.519 4 0.14 0 0 5 6.98 6.84 97.994 0.272 2.192 4.519 150 0 300 97.994 450 OD reading from True OD of sample spectro photome ter. 350 400 450 500 f(x) = − 3.21740546601867 x² + 86.0526870682699 x + 126.83170564342 Chart Ti
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BIOL 123 Practical Complement Mediated Lysis 2014
Transcript of BIOL 123 PRACTICAL Complement Mediated Lysis 2014 Data
Sheet1
Tube NoSheepGelatinDiluted guineaDistilled waterTube NoOD reading fromTrue OD of sample % lysiserythrocytes (l)Veronalpig serum(l)spectrophotometer.Buffer(l) made in step 3(GVB) (l)10.1590.0190.272115082515002150675300020.2930.1532.1923150525450041509750030.4550.3154.519515000975
40.1400
56.986.8497.994
0.2722.1924.5191500.27203002.19297.9944504.519100
53630757.572
linear = 539950y = -20.888x2 + 192.89x50-42575.5437.352polynomial = -42575.5
0.2721502.1923004.519450
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