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TOPIC:

TO STUDY THE EFFECTS OF TEMPERATURE ON THE ACTIVITY OF

SALIVARY AMYLASE

QUESTION:

You are required to plan out an investigation about the effect of temperature on the

reaction rate of enzyme activity. The activity of the enzyme ill be determined by

time ta!en for hydrolysis to be completed.

Your e"perimental planning should include the folloing:

• #im of investigation

• $tatement of hypothesis

• %ariables

•

&ist of apparatus and materials• Techique used

• '"perimental procedure or method

• Presentation of data

• Conclusion

(rite a report of the investigation)plan *+, mar!s-

ANSWER:

Aim: To study the effects of temperature on the activity of salivary amylase.

Problem stteme!t: (hat are the effects of temperature on the activity of salivary

amylase

H"#ot$esis: The optimum temperature for salivary amylase is /, 0C.

Vribles:

M!i#%lte& 'rible: Temperature

Res#o!&i!( 'rible: 1ate of reaction

Fi)e& 'ribles:  volume and concentration of starch suspension2 volume and

concentration of saliva suspension2 p3 of starch and saliva suspension.

A##rt%s: 4ea!ers2 test tube2 tripod stand2 ire gauze2 4unsen burner2 thermometer2

dropper2 glass rod2 syringes2 stopatch2 spotting tile and test tube rac!.

Mterils: +5 starch suspension2 saliva2 iodine solution2 ice cubes and distilled ater 

Te*$!i+%e:  1ecording the time ta!en for the complete hydrolysis of starch. The

 presence of starch is indicated by the iodine test.

Pro*e&%re:

+. 1inse the mouth ith distilled ater.

+

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6. Prepared the saliva suspension by splitting into a small bea!er and diluting the

saliva ith an amount of distilled ater equal to the amount of saliva.

/. Prepare five ater bath at the folloing temperature : 78C2 678C2 /,8C2 978C2

78C.

;. &abel to test tube  P  and Q.

9. Put ; ml of starch suspension in test tube P and + ml of saliva suspension intest tube