제 권 호46 1 새벽에 하나님이 도우시리로다 · 2019. 1. 5. · 2019 1 6년 월 일 ì·제 권 호46 1 새벽에 하나님이 도우시리로다 2019년도 한 해를
Antigen-Antibody Interactions : principles and Applications 미생물학교실 권 형 주.
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Transcript of Antigen-Antibody Interactions : principles and Applications 미생물학교실 권 형 주.
![Page 1: Antigen-Antibody Interactions : principles and Applications 미생물학교실 권 형 주.](https://reader035.fdocument.pub/reader035/viewer/2022062321/56649e4c5503460f94b42565/html5/thumbnails/1.jpg)
Antigen-Antibody Interactions : principles
and Applications
미생물학교실 권 형 주
![Page 2: Antigen-Antibody Interactions : principles and Applications 미생물학교실 권 형 주.](https://reader035.fdocument.pub/reader035/viewer/2022062321/56649e4c5503460f94b42565/html5/thumbnails/2.jpg)
Strength of Ag-Ab interactions
Ag + Ab Ag-Ab k1
K-1
Affinity: strength of the interaction between an antigen and an antibody Ka : association constant, affinity constant
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Determination of antibody affinity by equilibrium dialysis
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Ka = [Ab-Ag]/[Ab][Ag]= r
c(n-r)
r= the ratio of the concentration of bound ligandc= the concentration of free ligandn= the number of binding sites per antibody molecule = valency
rc
= Kan-Kar
Scatchard equation
Avidity : the strength of multiple interaction between a multivalent antibody and antigen
Affinity : Low HighAvidity : High LowValence : High Low
IgM IgG
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Cross-reactivity
ABO blood-group antigens : antigen (glycolipids) on RBC - Difference in the terminal residues of the sugars Blood group antibodies - elicited by microbial antigens
Streptococcus pyrogenes M protein Ab -- cross-react with myocardinal and skeletal muscle protein - Autoimmune diseases
Vaccines : Cowpox---smallpox express cross-reacting epitopes
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Surface Plasmon Resonance (SPR)
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Precipitation reactions
Immunoelectrophoresis of an antigen mixture
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Agglutination reactions
Hemagglutination
- Hemagglutination is used in blood typing- Bacterial agglutination is used to diagnose infection- Passive agglutination is useful with soluble antigens : Antigen-coated RBC - soluble Ag with RBC treated with tanic acid or chromium chloride
Radioimmunoassay
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Enzyme-Linked Immunosorbent Assay (ELISA)
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ELISPOT assay Western Blotting
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Immunoprecipitation
Immunofluorescence
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Flow Cytometry and Fluorescence
Flow Cytometry and Leukemia Typing