Analisis Sperma
-
Upload
sukandranaarya -
Category
Documents
-
view
79 -
download
20
description
Transcript of Analisis Sperma
![Page 1: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/1.jpg)
dr. Ety Retno Setyowati,M.Kes.,SpPK, MARS
![Page 2: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/2.jpg)
PENGERTIAN:Sperma adalah cairan/bahan setengah cair /setengah kental yang keluar melalui urethra pada pria dewasa yang sehat pada waktu mengalami orgasme (puncak kenikmatan seksual).
Nama lain : semen = air mani = ejakulatSperma 1. Spermatozoa = Sel mani 2. Plasma sperma
![Page 3: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/3.jpg)
TUJUAN :
Untuk mengetahui kuantitas dan kualitas
sperma.
PERSYARATAN
1. Abstinensi seksual (2 s/d 7 hari)
2. Evaluasi awal 2 sampel sperma
3. Pengumpulan sperma
- Masturbasi/onani
- Koitus Intruptus
![Page 4: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/4.jpg)
- Koitus kondomatus
- Refluks pascasanggama
- Pijatan Prostat (massage prostat)
- Dengan vibrator
4. Tempat penampungan bahan gelas
Tidak boleh dari logam/plastik
5. Pemeriksaan dilakukan 15-20 menit
6. Penanganan di Lab: harus hati-hati dan
petugas harus memakai pengaman (jas,
masker, sarung tangan).
![Page 5: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/5.jpg)
INDIKASI
1. Infertilitas = ketidak suburan
Primer (belum pernah),Sekunder (pernah
punya anak).
2. Penyakit-penyakit organ reproduksi pria (varikokel, hernia, kriptorhidi).
3. Interseks (sifat wanita > pria) Turner
pria, sindroma Klinefelter.
4. Homoseks
![Page 6: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/6.jpg)
5. Pengontrolan fertilitas pria pada KB
(pos vasektomi, KB hormonal).
6. Cek Up analisis sperma sebelum
perkawinan.
KOMPOSISI SPERMA
Merupakan campuran dari bahan/cairan yang dikeluarkan oleh bermacam-macam kelenjar traktus reproduksi pria.
![Page 7: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/7.jpg)
1. Kelenjar Testis (Tubuli Semeniferi Testis)
spermatozoa
2. Kelenjar Epididymis 0,3 CC
3. Kelenjar Vesika Seminalis 2-2,5 CC
4. Kelenjar Prostat 0,5 CC
5. Kelenjar Bulbourethralis (Cowper Gland)
dan kel Urethralis (Littre) 0,1-0,2 CC
![Page 8: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/8.jpg)
VARIABEL SPERMASperma mempunyai bagian-bagian,
sifat-sifat, serta keadaan-keadaan tertentu dan semua unsur itu menyusun sifat – sifat dan keadan khas sperma variabel sperma
Variabel Sperma :1. Variabel Dasar
(biologis)konsentrasi, motilitas, dan morfologi
spermatozoa2. Variabel Biokimia
![Page 9: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/9.jpg)
PEMERIKSAAN :
1. Makroskopis Likuefaksi Penampakan & warna sperma Bau sperma Volume sperma Konsistensi sperma pH sperma
![Page 10: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/10.jpg)
2. Mikroskopis Pemeriksaan per lapangan pandang - Sperma diteteskan pada obyek
glas,lalu ditutup dengan cover glas dan dilihat di bawah mikroskop cahaya pembesaran 400 X - Dievaluasi spermatozoa /plp : 25-50/plp; 50-100/plp; 100-200/plp; 300-400/plp; 400-500/plp.
![Page 11: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/11.jpg)
MAKROSKOPIS MIKROSKOPIS
• LIQUEFACTION LIQUEFACTION
• WARNAWARNA
• BAUBAU
• VISKOSITASVISKOSITAS
• VOLUMEVOLUME
• pHpH
• LIQUEFACTION LIQUEFACTION
• WARNAWARNA
• BAUBAU
• VISKOSITASVISKOSITAS
• VOLUMEVOLUME
• pHpH
• JUMLAHJUMLAH• MOTILITASMOTILITAS• AGLUTINASIAGLUTINASI• ELEMEN ELEMEN
SELULER SELULER NONSPERMANONSPERMA
• JUMLAHJUMLAH• MOTILITASMOTILITAS• AGLUTINASIAGLUTINASI• ELEMEN ELEMEN
SELULER SELULER NONSPERMANONSPERMA
![Page 12: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/12.jpg)
MIKROSKOPIS LANJUTAN
• VITALITAS SPERMA VITALITAS SPERMA • PENCACAHAN SPERMAPENCACAHAN SPERMA• MORFOLOGIMORFOLOGI• LEUKOSITLEUKOSIT• ANTISPERMA ANTIBODI (MAR TESTANTISPERMA ANTIBODI (MAR TEST
• VITALITAS SPERMA VITALITAS SPERMA • PENCACAHAN SPERMAPENCACAHAN SPERMA• MORFOLOGIMORFOLOGI• LEUKOSITLEUKOSIT• ANTISPERMA ANTIBODI (MAR TESTANTISPERMA ANTIBODI (MAR TEST
![Page 13: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/13.jpg)
Sel-sel bulat (sel germinal/leukosit) Agglutinasi spermatozoa Benda-benda khusus sperma (epitel, kristal-kristal, lemak, benda prostat, bakteri dan protozoa)
Menghitung spermatozoa Analisis karakteristik morfologi spermatozoa
![Page 14: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/14.jpg)
AGLUTINASI SPERMATOZOA
![Page 15: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/15.jpg)
Motilitas spermatozoa
a. Gerak sangat cepat, maju lurus
kedepan
b. Gerak kurang cepat/pelan maju
lurus kedepan
c. Gerak di tempat /zigzag
d. Diam / tidak bergerak
![Page 16: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/16.jpg)
Menghitung Spermatozoa
Bila plp < 100 10 X, >100 20X 10 X sedot sperma 1 encerkan 11 20 X sedot sperma 0,5 encerkan 11 Hitung dalam Hemositometer (Improve Neubauer), pada bagian sentral. Hasil perhitungan : N X 10.000/20.000Konsentrasi spermatozoa juta/ml
![Page 17: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/17.jpg)
Kamar hitung Hemositometer
![Page 18: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/18.jpg)
Bagian sentral kamar hitung
![Page 19: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/19.jpg)
Analisis karakteristik morfologi spermatozoa
Untuk memeriksa morfologi spermatozoa harus dibuat preparat hapusan, dan setelah diwarnai dan dikeringkan dilihatDibawah mikroskop dengan pembesaran 400 x.Kemudian diidentifikasi bentuk-bentukabnormal spermatozoa
![Page 20: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/20.jpg)
NOMENKLATURE
1. Normozoospermia
2. Oligozoospermia
3. Ekstrem oligozoospermia
4. Asthenozoopermia
![Page 21: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/21.jpg)
5.Teratozoospermia
6. Oligoasthenozoospermia (OA)
7. Oligoasthenoteratozoospermia (OAT)
8. Polizoospermia
![Page 22: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/22.jpg)
9. Azoospermia
10. Nekrozoospermia
1. Kriptozoospermia
12. Aspermia
![Page 23: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/23.jpg)
-Membuat preparat hapus sperma- Fiksasi dikeringkan pewarnaan- Pewarnaan : Giemza, Meyer, Fast Green, Wright, Steeno, Bryan/Leishman, dan Papanicolou- Pembacaan di bawah mikroskop cahaya dengan pembesaran 1000 kali- Penentuan bentuk-bentuk spermatozoa normal dan abnormal.
![Page 24: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/24.jpg)
Bentuk Spermatozoa Normal :
- Kepala oval, akrosom meliputi 1/3 nya,
Panjang 3-5 mikron, lebar 2-3 mikron
(1/2-2/3 panjang kepala).
- Midpiece langsing (< ½ lebar kepala),
panjang 2 kali panjang kepala, berada
satu garis dengan sumbu panjang
kepala.
- Ekor batas tegas berupa garis, panjang
9 kali panjang kepala
![Page 25: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/25.jpg)
Macam abnormalitas kepala :
• Makro 25% lebih besar
• Mikro 25% lebih kecil
• Kepala Taper
• Kepala piri tetesan air mata
• Kepala amorf (terato)
• Kepala round (bulat)
• Kepala ganda
• Kepala pin tidak ada bentuk kepala
Sitoplasma droplet, midpiece & ekor abn.
![Page 26: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/26.jpg)
A & B: SPERMATOZOA NORMAL, C: KEPALA MAKRO, D KEPALA MIKRO, E,F KEPALA TAPER
![Page 27: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/27.jpg)
G:KEPALA PIRI, H:KEPALA DOBEL, I:KEPALA AMORF, J: KELAINAN EKOR (SPIRAL),K,L:DROPLET SITOPLASMA
![Page 28: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/28.jpg)
A:spermatogonium, B:Spermatosit primer, C:Spermatosit sekunder, D,E,F:Spermatid (Sab).
![Page 29: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/29.jpg)
A,B,C,D: Lekosit polimorfonuklear (PMN)
![Page 30: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/30.jpg)
E : Limfosit, F: sel Epitel
![Page 31: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/31.jpg)
![Page 32: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/32.jpg)
NORMAL
![Page 33: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/33.jpg)
![Page 34: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/34.jpg)
PIRIFORM
![Page 35: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/35.jpg)
LEPTO
![Page 36: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/36.jpg)
DOUBLE HEAD
![Page 37: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/37.jpg)
MIDPIECE EFECT
![Page 38: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/38.jpg)
MACRO - MICRO
![Page 39: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/39.jpg)
GERM CELL
![Page 40: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/40.jpg)
TAIL DEFECT
![Page 41: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/41.jpg)
SPERMATID
![Page 42: Analisis Sperma](https://reader035.fdocument.pub/reader035/viewer/2022082210/55cf8fc1550346703b9f77e5/html5/thumbnails/42.jpg)
42
Terima Kasihk