An Introduction to the Sydney University Proteome Research Unit
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Transcript of An Introduction to the Sydney University Proteome Research Unit
An Introduction to the An Introduction to the Sydney University Sydney University Proteome Research UnitProteome Research Unit
Dr Ben CrossettDr Ben Crossett
The History… • Opened in July 2004 with $2m
MNRF grant & USyd funding• Centre of excellence for
proteomics• Level 2, Building G08• People come from: SMB, SoBS,
Medicine, VetSci, Agriculture, • ...as well as: MQ, UNWS, UWS,
UTS, Brisbane, UK, USA, Germany etc...
To gel or...
...or not to gel
The Results…
• Whole protein mass• Identify proteins• Labelling QC• Changes in protein
abundance, e.g. DIGE or iTRAQ
• Mapping post-translational modifications
• MS ‘western blots’
2000.0 7600.2 13200.4 18800.6 24400.8 30001.0
Mass (m/z)
1.2E+4
0
10
20
30
40
50
60
70
80
90
100
% In
ten
sity
Voyager Spec #1[BP = 23504.3, 12073]
23505.27
23452.36
23401.56
23585.13
23622.9023659.68
23700.26
23744.51
11742.36
23319.85
2014.3623799.20
2280.91
3014.85
2466.6411846.38
2362.002856.22
23162.722979.573832.54 11936.27
22607.404491.42
5796.63 25204.0117286.42
Accessing SUPRU • Contract research: samples
analysed for fixed fee • Research hotel: come and work
in SUPRU • Collaborative research projects• Short courses &
under/postgraduate lectures
• Contact us at:sydney.edu.au/[email protected]