8/13/2019 Amicon Pro Diafiltration

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Choose the direct route. Replace old techniques with the pro.Amicon® Pro Purification System

EMD Millipore is a division of Merck KGaA, Darmstadt, Germany

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Competitor 0.5 mL affinity-based spin column Amicon® Pro Device

E1 (0.5 mL) E2 (0.5 mL) E3 (0.5 mL) E1 (1 mL) E2 (1 mL)Total (1.5 mL)

Elution without dilution – simultaneous elution and

concentration with the Amicon® Pro system.

1 Spin – efficient clearance of non-specific protein with one

wash.

Gravi ty Column Amicon® Pro dev ice Compet itor Spin

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Wash 3

All-In-ONE:Purify, concentrate and exchange

buffer in 1.5 hours; 5 spins.

Workingwith

Proteins?

AbLabeling

1 hr, 3 spins

Dialysis20 min, 1 spin

Desalting20 min, 1 spin

Continuous buffer exchange:Novel design enables 1000 fold

(99.99%) desalting while maintainingprotein integrity

AffinityPurification

1 hr, 4 spins

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