Alu I
2
M AluI Hi nf I Hi nfI+EcoRI I AfaI Msp I 200 500 100 bp TGGAAGGACAGATTCGCCATTAC AAATGGTGTTGCTCGAGGGCTTTTATATCTTCATCAAGACTCACGGTTTAGGATAATCCACAG GGATTTGAAACCAGGTAACATTTTGCTTGATAAATATATGATCCCAAAGATCTCGGATTTTGGGATGGCCAGAATCTTTGCAAGGG ACGAAACTCAAGCTAGGACAGACAATGCGGTCGGAACTTA Gtaagcaagtctttgaagatattttcttacccataactctatagaactcatgacctctaagttcattttttttgctactcag CGGGTACATGTCTCCGGAGTACGCAATGGATGGGGTAATCTCGGAAAAAACAGATGTTTTCAGTTTTGGAGTCATAGTTCTTGAAA TTGTTAGTGGAAAAAGGAATAGAGGATTCTACCAGGTGAACCCTGAAAACAATCTTCTAAGCTAT Gtaagttaaagaaccaataatattctatctactctcgaaattgctaaaacacttttttttaacaacgctaaaacaattgaaatgct tttatttttataaacag GCATGGAGTCATTGGGCGGAGGGAAGAGCGCTAGAAATCGTAGATCCAGTCATCGTAGATCCATTGGCATCTCTGCCATCAACATT TCAACCAAAAGAAGTCCTAAAATGCATACAAATTGGTCTATTGTGTATTCAAGAACGTGCGGAGCACAGACCAACGATGTCGTCGG TGGTTTGGATGCTTGGAAGTGAAGCAACAGAGATTCCTCAGCCTAAACCGCCAGTTTATTGTCTCATAGCAAGTTATTATGCAAAT AATCATTCCTCAAGTAGGCAATTCGACGACGATGAATCCTGGACGGTGAACAAGTACACCTGCTCAGTCATCGATGCCCGGTAA TATGAAAGC Supplementary Fig. 1 PCR amplification of SRK class I gene (899 bp) of rapid cycle B. oleracea ‘ TO1000DH3’ using KD5 and KD8 primers and subsequent partial digestion with restriction enzymes. The gene sequence is mentioned below with primers and restriction sites.
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Hin fI+ Eco RII. Msp I. Hin fI. Alu I. Afa I. M. bp. 500. 200. 100. - PowerPoint PPT Presentation
Transcript of Alu I
M AluI
HinfI
HinfI+EcoR
IIAfaI
MspI
200
500
100
bp
TGGAAGGACAGATTCGCCATTACAAATGGTGTTGCTCGAGGGCTTTTATATCTTCATCAAGACTCACGGTTTAGGATAATCCACAGGGATTTGAAACCAGGTAACATTTTGCTTGATAAATATATGATCCCAAAGATCTCGGATTTTGGGATGGCCAGAATCTTTGCAAGGGACGAAACTCAAGCTAGGACAGACAATGCGGTCGGAACTTAGtaagcaagtctttgaagatattttcttacccataactctatagaactcatgacctctaagttcattttttttgctactcagCGGGTACATGTCTCCGGAGTACGCAATGGATGGGGTAATCTCGGAAAAAACAGATGTTTTCAGTTTTGGAGTCATAGTTCTTGAAATTGTTAGTGGAAAAAGGAATAGAGGATTCTACCAGGTGAACCCTGAAAACAATCTTCTAAGCTATGtaagttaaagaaccaataatattctatctactctcgaaattgctaaaacacttttttttaacaacgctaaaacaattgaaatgcttttatttttataaacagGCATGGAGTCATTGGGCGGAGGGAAGAGCGCTAGAAATCGTAGATCCAGTCATCGTAGATCCATTGGCATCTCTGCCATCAACATTTCAACCAAAAGAAGTCCTAAAATGCATACAAATTGGTCTATTGTGTATTCAAGAACGTGCGGAGCACAGACCAACGATGTCGTCGGTGGTTTGGATGCTTGGAAGTGAAGCAACAGAGATTCCTCAGCCTAAACCGCCAGTTTATTGTCTCATAGCAAGTTATTATGCAAATAATCATTCCTCAAGTAGGCAATTCGACGACGATGAATCCTGGACGGTGAACAAGTACACCTGCTCAGTCATCGATGCCCGGTAATATGAAAGCSupplementary Fig. 1 PCR amplification of SRK class I gene (899 bp) of rapid cycle B. oleracea ‘ TO1000DH3’ using KD5 and KD8 primers and subsequent partial digestion with restriction enzymes. The gene sequence is mentioned below with primers and restriction sites.
Supplementary Fig. 2 Alignment of nucleotide sequences of the kinase domain of TO1000DH3 and BoS-7. Numbers on the right margin indicate the positions of base pairs. The identical amino acids are marked in dark background, and dissimilar in light
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
TO1000DH3
BoS-7
