Aktivni transport preko celičnih membran ATP-gnane črpalkebio.ijs.si/~krizaj/group/Predavanja...
Transcript of Aktivni transport preko celičnih membran ATP-gnane črpalkebio.ijs.si/~krizaj/group/Predavanja...
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Aktivni transport preko celičnih membran
ATP-gnane črpalke
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MCB - Chapter 15
The four classes of ATP-powered transport proteins
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MCB - Chapter 15
Model of mechanism of action of muscle-type Ca2+ ATPase(SERCA)
Km = 10-7 M
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MCB - Chapter 15
Schematic structural model for the α-subunit of muscle Ca2+ ATPase
Inhibited by thapsigargin
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Na+/K+ ATPase is the most important ion pumpin formation of the PM potential
Lehninger - Chapter 12
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MCB - Chapter 15
Na+/K+ ATPase and its mode of action
Inhibited by ouabain
KmNa+ = 0.6 mM
KmK+ = 0.2 mM
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MCB - Chapter 15
Acidification of the stomach lumen by P-class H+/K+ ATPase in parietal cells
pH 1pH 7
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Structural comparison of the V- and F-ATPases
Kawasaki-Nishi, S. et al. (2003) FEBS Lett. 545, 76-85
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Characteristics of V-ATPase subunits
Forgac, M. (2007) Nature Rev. MCB 8, 917-929
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Kawasaki-Nishi, S. et al. (2003) FEBS Lett. 545, 76-85
Electron micrographs of the V0 and V1V0 complexes
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Kawasaki-Nishi, S. et al. (2003) FEBS Lett. 545, 76-85
Structure and function of the V0 domain
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Forgac, M. (2007) Nature Rev. MCB 8, 917-929
Mechanism of proton transport by the V-ATPase
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Function of V-ATPases in exo/endocytosis and intracellular targeting
Forgac, M. (2007) Nature Rev. MCB 8, 917-929
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Functions of plasma membrane V-ATPases
Nishi, T. and Forgac, M. (2002) Nat. Rev. Mol. Cell Biol. 3, 94-103
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MCB - Chapter 15
V-class ATPases in toad bladder epithelial cells
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Regulation of V-ATPases
Cipriano, D.J. et al. (2008) BBA 1777, 599-604
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Lehninger - Chapter 12
Cotransport systemsdriven by gradients of Na+ and H +
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MCB - Chapter 15
Transport of the glucose (or an amino acid) from the intestinal lumen (or urine) into the blood
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ABC transporters
- Bacterial plasma membrane permeases
- Multidrug-resistance proteins (MDR1, MDR2)
- Cystic fibrosis transmembrane regulator (CFTR) protein
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MCB - Chapter 15
PM of many bacteria contain differentABC transporters - permeases
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MCB - Chapter 15
Schematic structural model of mammalianmultidrug-resistance (MDR) transport protein -- a general structural model of ABC transporters
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MCB - Chapter 15
Possible mechanisms of action of the MDR1 protein
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Cystic fibrosis transmembrane-conductance regulator (CFTR)protein is a Cl- channel
Lehninger - Chapter 12
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MCB - Chapter 15
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Recommended reading:
Lodish, H. et al. (2000): Transport across cell membranes. In: Molecular Cell Biology, 4th edition, W.H. Freeman and Company, New York.
Nelson, D.L. and Cox, M.M. (2000): Biological membranes and transport. In: Lehninger Principles of Biochemistry, 3th edition, Worth Publishers, New York.
Alberts, B. et al. (2002): Membrane transport of small molecules and the electrical properties of membranes. In: Molecular Biology of the Cell, 4th edition, Garland Science, New York.
Nishi, T. and Forgac, M. (2002): The vacuolar (H+)-ATPases – nature`s most versatile proton pumps. Nat. Rev. Mol. Cell Biol. 3, 94-103.
Kawasaki-Nishi, S., Nishi, T. and Forgac, M. (2003): Proton translocation driven by ATP hydrolysis in V-ATPases. FEBS Lett. 545, 76-85.
Forgac, M. (2007): Vacuolar ATPases: rotary proton pumps in physiology and pathophysiology Nature Rev. MCB 8, 917-929.
Cipriano, D.J. et al. , M. (2008): Structure and regulation of the vacuolar ATPases. Biochim. Biophys. Acta 1777, 599-604.