A new On-line Solid Phase extraction system from Waters...The Anatomy of the Acquity OSM Acquity...
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©2009 Waters Corporation | COMPANY CONFIDENTIAL ©2011 Waters Corporation – COMPANY CONFIDENTIAL A new On-line Solid Phase extraction system from Waters Acquity OSM Denis Calnan Clinical/Forensic Business Specialist (Northern Europe) Båstad Nordics Symposium 2011
Transcript of A new On-line Solid Phase extraction system from Waters...The Anatomy of the Acquity OSM Acquity...
©2009 Waters Corporation | COMPANY CONFIDENTIAL©2011 Waters Corporation – COMPANY CONFIDENTIAL
A new On-line Solid Phase
extraction system
from Waters
Acquity OSM
Denis Calnan
Clinical/Forensic Business
Specialist (Northern Europe)
Båstad Nordics Symposium 2011
©2009 Waters Corporation | COMPANY CONFIDENTIAL©2011 Waters Corporation – COMPANY CONFIDENTIAL
Agenda
Acquity Online Solid Phase Extraction Manager (OSM)
How was it developed?
Benefits of online SPE
Hardware
• Questions/ Discussion
©2011 Waters Corporation – COMPANY CONFIDENTIAL 3
Introducing Acquity Online SPE Manager (OSM)
“A UPLC®-enabled online solid phase
extraction system”
©2011 Waters Corporation – COMPANY CONFIDENTIAL 4
What is Acquity OSM?
The Acquity OSM
— A device to provide UPLC-enabled online SPE
— OSM (online SPE Manager)
— Part of the Acquity online SPE System
o Acquity Sample Manager
o Acquity Binary Solvent Manager
o Acquity Column Manager – A
o Acquity TQ Detector
(or Xevo TQ MS + TQS)
©2011 Waters Corporation – COMPANY CONFIDENTIAL 5
How did we get here?
Online SPE investigations began in Jan 2006
— Spark Holland Symbiosis Pharma
o Plasma Free Metanephrines
o Urinary Free Cortisol
o Testosterone & TDM
Attractive features
— Processing of biological matrices
— New cartridge for every sample
— Decent results & publications
— Easier than any Waters solution at the time
©2011 Waters Corporation – COMPANY CONFIDENTIAL 6
Undesirable Features of Spark Symbiosis and other HPLC based on-line SPE system
Awkward to deploy in the field
— Sales & support of non-Waters system
Size & Configuration
— Large sample management module
— Limited solvent capacity (mobile phases)
— Lengthy fluidic pathways
— Separate column oven (control?)
HPLC pressures in a UPLC world
©2011 Waters Corporation – COMPANY CONFIDENTIAL 7
What are the attractions of online SPE?
All of the benefits of standard SPE
— Sample clean-up
— Sample concentration
— Automation
Online SPE
— Sample efficiency
o Off-line SPE (plates, cartridges) always loses something
o Online, everything on the cartridge is passed to the column
— Parallel processing of samples
— Multiple Modes of Operation
©2011 Waters Corporation – COMPANY CONFIDENTIAL 8
The Anatomy of the Acquity OSM
Acquity Sample Manager
• 2 samples trays• Variable injection volume (max 200µL)• (optional sample organizer)
Acquity BinarySolvent Manager
• 4 Solvent Selection• 15,000 psi
Acquity Column Manager
• 4 column capacity
•Modified access characteristics
Acquity Online SPE Manager
Acquity TQ Detector
“A UPLC®-enabled online solid phase
extraction system”
©2011 Waters Corporation – COMPANY CONFIDENTIAL 9
A closer look at Acquity OSM
Left Clamp(Elution)
Right Clamp(Loading)
High Pressure Dispenser (HPD) SPE Solvent
Inlets
Valving to allow switching of
modes
Gripper to move cartridges to & form clamps
2 x 96-well plates/trays of cartridges
©2011 Waters Corporation – COMPANY CONFIDENTIAL 10
Tray of SPE cartridges
©2011 Waters Corporation – COMPANY CONFIDENTIAL 11
Amount Of Phospholipids Present In Whole Blood Before And After Extraction Using The OSM
whole blood after extraction
Whole blood before extraction
©2011 Waters Corporation – COMPANY CONFIDENTIAL 12
Ion Suppression For Whole Blood Extracted Using The OSM
Extracted whole blood
50% methanol (aqueous)
©2011 Waters Corporation – COMPANY CONFIDENTIAL 13
IATDMCT 2011 poster
©2011 Waters Corporation – COMPANY CONFIDENTIAL 14
Benefits of Acquity OSM
A single vendor solution with traceability features
— Hardware, chemistries & integrated software
Automated online SPE – all of the benefits of SPE and more
— Removing the human factor
o Time, errors and injury
o Parallel processing & improved time-to-first result
— Sample Clean-up
— Sample Concentration
Consolidation of methods means system flexibility
— Reduction in column changes
— Reduction in mobile phase changes
— Reduction in SPE solvent handling
— Other online systems are dedicated to a particular analysis
©2011 Waters Corporation – COMPANY CONFIDENTIAL 15
Conclusion
Single cartridge per sample – very low risk of carry-over
Operates in UPLC mode ie, faster runtimes, enhanced
resolution + increased sensitivity
Options of up to 23 solvents for SPE steps
Flexible system with multiple chemistry options will allow
seamless switching between methods
Complete software control within MassLynx
ISO 13485 and EU IVDD 98/79/EC
Currently in Demo labs and ―Seed evaluation‖ sites
Available Q2 2012
