61 DAFTAR PUSTAKA 1. WHO. Dibalik Angka Pengkajian Kematian ...
Transcript of 61 DAFTAR PUSTAKA 1. WHO. Dibalik Angka Pengkajian Kematian ...
61
DAFTAR PUSTAKA
1. WHO. Dibalik Angka Pengkajian Kematian Maternal dan Komplikasi untuk
Mendapatkan Kehamilan yang Lebih Aman. Jakarta, 2007:172.
2. RI KK. Pusat data dan informasi: Situasi Kesehatan Ibu. Jakarta Selatan,
2014.
3. Say L, Chou D, Gemmill A, et al. Global causes of maternal death: a WHO
systematic analysis. The Lancet Global Health;2:e323-e333.
4. Osungbade KO, Ige OK. Public health perspectives of preeclampsia in
developing countries: implication for health system strengthening. Journal of
pregnancy 2011;2011:481095.
5. Statistics by country for preeclampsia:(diakses pada 30 November 2014).
Diunduh dari http://www.wrongdiagnosis.com/p/preeclampsia/stats-
country.htm.
6. Katz DL, Gnanaraj J, Treu JA, Ma Y, Kavak Y, Njike VY. Effects of egg
ingestion on endothelial function in adults with coronary artery disease: a
randomized, controlled, crossover trial. American heart journal
2015;169:162-9.
7. F. Gary Cunningham KJL, Steven L. Bloom, John C. Hauth, Dwight J.
Rouse, Catherine Y. Spong. Pregnancy Hypertension. In: William Obstetrics,
23rd ed. New York: McGraw-Hill, 2010.
8. Schlembach D. Pre-eclampsia--still a disease of theories. Fukushima journal
of medical science 2003;49:69-115.
9. Borges K, Hassan N, Hussain R, Akhtar MT. Effects of variation in umbilical
artery resistive index on placental morphology and birth weight in pregnancy
induced hypertension. Journal of Ayub Medical College, Abbottabad: JAMC
2013;25:23.
10. Di Naro E, Ghezzi F, Raio L, Franchi M, D’Addario V. Umbilical cord
morphology and pregnancy outcome. European Journal of Obstetrics &
Gynecology and Reproductive Biology 2001;96:150-157.
11. Junek T, Baum O, Läuter H, Vetter K, Matejevic D, Graf R. Pre-eclampsia
associated alterations of the elastic fibre system in umbilical cord vessels.
Anatomy and embryology 2000;201:291-303.
12. Barnwal M, Rathi S, Chhabra S, Nanda S. Histomorphometry of Umbilical
Cord and its Vessels in Pre-Eclampsia as Compared to Normal Pregnancies.
Nepal Journal of Obstetrics and Gynaecology 2013;7:28-32.
13. Dekker GA, Sibai BM. Etiology and pathogenesis of preeclampsia: current
concepts. American journal of obstetrics and gynecology 1998;179:1359-
1375.
62
14. Blanco MV, Vega HR, Guerri-Guttenberg RA, et al. Histopathology and
histomorphometry of umbilical cord blood vessels. Findings in normal and
high risk pregnancies. Artery Research 2011;5:50-57.
15. Stehbens* WE, Wakefield JSJ, Gilbert-Barness E, Zuccollo JM.
Histopathology and ultrastructure of human umbilical blood vessels. Fetal &
Pediatric Pathology 2005;24:297-315.
16. Kim KS, Kim YS, Lim JI, Jung MH, Park HK. Nanoscale imaging of
morphological changes of umbilical cord in pre-eclampsia. Microscopy
research and technique 2012;75:1445-51.
17. Espinoza J. Uteroplacental ischemia in early‐and late‐onset pre‐eclampsia: a
role for the fetus? Ultrasound in Obstetrics & Gynecology 2012;40:373-382.
18. Obstetricians ACo, Gynecologists. Hypertension in pregnancy. Report of the
American College of Obstetricians and Gynecologists’ Task Force on
Hypertension in Pregnancy. Obstetrics and gynecology 2013;122:1122.
19. Frias AE, Jr., Belfort MA. Post Magpie: how should we be managing severe
preeclampsia? Current opinion in obstetrics & gynecology 2003;15:489-95.
20. Kesehatan DJBU. Pedoman Nasional Pelayanan Kedokteran Diagnosis dan
Tatalaksana Preeklampsia. Jakarta: Kementerian Kesehatan RI, 2013.
21. Vanderlelie J. Placental Oxidative Stress in Preeclampsia. Science Medicine.
Melbourne: Griffith, 2005:40.
22. Rogers BB, Bloom SL, Leveno KJ. Atherosis revisited: current concepts on
the pathophysiology of implantation site disorders. Obstetrical &
gynecological survey 1999;54:189-195.
23. Lindheimer MD, Taler SJ, Cunningham FG. Hypertension in pregnancy.
Journal of the American Society of Hypertension : JASH 2008;2:484-94.
24. Bisseling TM, Maria Roes E, Raijmakers MT, Steegers EA, Peters WH,
Smits P. N-acetylcysteine restores nitric oxide-mediated effects in the
fetoplacental circulation of preeclamptic patients. American journal of
obstetrics and gynecology 2004;191:328-33.
25. van Pampus MG, Aarnoudse JG, 15805804 AN, et al. Long-term outcomes
after preeclampsia. Clinical obstetrics and gynecology 2005;48:489-94.
26. Bellamy L, Casas JP, Hingorani AD, Williams DJ. Pre-eclampsia and risk of
cardiovascular disease and cancer in later life: systematic review and meta-
analysis. Bmj 2007;335:974.
27. Rana J, Ebert GA, Kappy KA. Adverse perinatal outcome in patients with an
abnormal umbilical coiling index. Obstet Gynecol 1995;85:573-7.
28. Bańkowski E, Sobolewski K, Romanowicz L, Chyczewski L, Jaworski S.
Collagen and glycosaminoglycans of Wharton's jelly and their alterations in
EPH-gestosis. European Journal of Obstetrics & Gynecology and
Reproductive Biology 1996;66:109-117.
63
29. Kurita M, Hasegawa J, Mikoshiba T, et al. Ultrasound evaluation of the
amount of Wharton's jelly and the umbilical coiling index. Fetal diagnosis
and therapy 2009;26:85-9.
30. Kuswani L. Perbandingan Indeks Koil Tali Pusat Terhadap Luaran Berat
Badan Bayi Lahir. 2011.
31. Ferguson VL, Dodson RB. Bioengineering aspects of the umbilical cord.
European journal of obstetrics, gynecology, and reproductive biology
2009;144 Suppl 1:S108-13.
32. Bimpong S. Quantitative Evaluation of Umbilical Cord and Placental Indices
and Pregnancy Outcome. Theoretical and Applied Biology. Ghana: Kwame
Nkrumah, 2012.
33. Sadler TW. Langman's Medical Embryology: Lippincott Williams & Wilkins,
2011.
34. Inan S, Sanci M, Can D, Vatansever S, Oztekin O, Tinar S. Comparative
morphological differences between umbilical cords from chronic
hypertensive and preeclamptic pregnancies. Acta Medica Okayama
2002;56:177-186.
35. Ilie C, Hrubaru N, Ilie R, et al. Histological modifications of the umbilical
cord in pregnancy induced hypertension. I GENETICS 1915:12.
36. Baptiste-Roberts K, Salafia CM, Nicholson WK, Duggan A, Wang N-Y,
Brancati FL. Maternal risk factors for abnormal placental growth: the national
collaborative perinatal project. BMC pregnancy and childbirth 2008;8:44.
37. S. P. Ilmu kebidanan. Jakarta: EGC, 2010.
38. Botdorf J, Chaudhary K, Whaley-Connell A. Hypertension in cardiovascular
and kidney disease. Cardiorenal medicine 2011;1:183.
39. Møller S, Henriksen J. Cirrhotic cardiomyopathy: a pathophysiological
review of circulatory dysfunction in liver disease. Heart 2002;87:9-15.
40. Eadara Murthy IR M. Review: diabetes and pregnancy. Diabetologia croatica.
2002:131-146.
41. Wiknjosastro. Ilmu kebidanan. Jakarta: EGC, 2005.
42. Eclampsia (seizure) and preeclampsia, 2012.
43. Irnawati MH W. Ibu hamil perokok pasif sebagai faktor risiko bayi berat lahir
rendah. Jurnal Gizi Klinik Indonesia. 2011:54-59.
44. de Laat MW, Nikkels PG, Franx A, Visser GH. The Roach muscle bundle
and umbilical cord coiling. Early human development 2007;83:571-4.
45. Machin GA, Ackerman J, Gilbert-Barness E. Abnormal umbilical cord
coiling is associated with adverse perinatal outcomes. Pediatric and
developmental pathology : the official journal of the Society for Pediatric
Pathology and the Paediatric Pathology Society 2000;3:462-71.
64
46. Jasovic-Siveska E, Jasovic V, Stoilova S. Previous pregnancy history, parity,
maternal age and risk of pregnancy induced hypertension. Bratislavske
lekarske listy 2011;112:188-91.
47. Luo Z-C, An N, Xu H-R, Larante A, Audibert F, Fraser WD. The effects and
mechanisms of primiparity on the risk of pre-eclampsia: a systematic review.
Paediatric and perinatal epidemiology;21:36-45.
48. Hardiyanti MD, Pramono BA. FAKTOR-FAKTOR YANG
BERPENGARUH TERHADAP LUARAN MATERNAL DAN
PERINATAL PADA IBU HAMIL DI USIA TUA Studi Kasus di RS.
Adhyatma Semarang selama Tahun 2012: Faculty of Medicine Diponegoro
University, 2014.
49. WIyono S. Hubungan antara kadar serum ferritin dengan tebal dan diameter
arteri umbilikalis pada preeklampsia berat dan hamil normotensi. 2014.
50. Weissman A, Jakobi P. Sonographic measurements of the umbilical cord in
pregnancies complicated by gestational diabetes. Journal of ultrasound in
medicine 1997;16:691-694.
51. Ekapatria C, Sabarudin U, Sasotya S. Placental Growth Factor Level is Lower
in Early-Onset Preeclampsia, while Tumor Necrosis Factor Alpha Level does
not Show any Difference between Early and Late Onset Preeclampsia.
Indonesian Journal of Obstetrics and Gynecology 2013;36:181-184.
52. Valensise H, Vasapollo B, Gagliardi G, Novelli GP. Early and late
preeclampsia two different maternal hemodynamic states in the latent phase
of the disease. Hypertension 2008;52:873-880.
53. Soto E, Romero R, Kusanovic JP, et al. Late-onset preeclampsia is associated
with an imbalance of angiogenic and anti-angiogenic factors in patients with
and without placental lesions consistent with maternal underperfusion.
Journal of Maternal-Fetal and Neonatal Medicine 2012;25:498-507.
54. Espinoza J, Uckele JE, Starr RA, Seubert DE, Espinoza AF, Berry SM.
Angiogenic imbalances: the obstetric perspective. American journal of
obstetrics and gynecology 2010;203:17. e1-17. e8.
55. LaMarca B, Brewer J, Wallace K. IL-6-induced pathophysiology during pre-
eclampsia: potential therapeutic role for magnesium sulfate? International
journal of interferon, cytokine and mediator research: IJIM 2011;2011:59.
56. Sugimoto J, Romani AM, Valentin-Torres AM, et al. Magnesium decreases
inflammatory cytokine production: a novel innate immunomodulatory
mechanism. The Journal of Immunology 2012;188:6338-6346.
57. Smith P, Anthony J, Johanson R. Nifedipine in pregnancy. BJOG: An
International Journal of Obstetrics & Gynaecology 2000;107:299-307.
65
58. Belfort MA, Saade GR, Suresh M, Johnson D, Vedernikov YP. Human
umbilical vessels: responses to agents frequently used in obstetric patients.
American journal of obstetrics and gynecology 1995;172:1395-1403.
59. Immunohistochemistry guide:(diakses pada 12 Juni 2015). Diunduh dari
http://www/.mdbioproducts.com/resources/protocols/immunohistochemistry.
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Lampiran 1. Ethical Clearance
67
Lampiran 2. Izin Penelitian
68
Lampiran 3. Informed Consent
69
Lampiran 3. Informed Consent (lanjutan)
70
Lampiran 3. Informed Consent (lanjutan)
71
Lampiran 3. Informed Consent (lanjutan)
v
v
72
Lampiran 4. Prosedur Pembuatan Preparat
Judul : Perbedaan Diameter Lumen Arteri Umbilikalis Pada
Preeklampsia Berat dan Kehamilan Normotensi
Tempat : Laboratorium Sentral RSUP Dr. Kariadi
1. Potong tali pusat sepanjang 2 cm dari bagian fetal
masukkan ke dalam container berisi formalin
dengan perbandingan volume jaringan: formalin
1:10beri identitas bawa ke laboratorium
Patologi Anatomi
2. Jaringan mentah dipotong menjadi 3 bagian (dipilih
yang mencurigakan) lalu masukkan ke dalam kaset
dan ke dalam wadah berisi formalin 10%
3. Processing jaringan selama 17, 5 jam:
Kaset dimasukkan ke dalam keranjang (1 keranjang
bisa memuat 80 kaset) masukkan pada alat
processing jaringan Microm STP 120, yang terdiri
atas beberapa tabung dengan lama masing-masing
waktu perendaman:
- Formalin 10% 12 jam
- Formalin 10% 22 jam
- Alkohol 70 % 1 jam
- Alkohol 80% 1 jam
- Alkohol 96%2 jam
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- Etanol2 jam
- Xylol1 jam
- Parafin 12 jam
- Parafin 22 jam
- Parafin 32 jam
Tujuan processing:
- Menghilangkan cairan pada jaringan (dehidrasi
dg alkohol bertingkat yakni : alkohol 70%,
80%, 96% dan etanol)
- Xylol berfungsi untuk clearing
- Parafin untuk mematangkan jaringan
2 Membuat blok jaringan yang bermula dari jaringan
matang, kemudian dengan alat histocenter: dipanas-
dinginkan memakai cairan parafin dibekukan
3. Hasil blok jaringan dipotong dengan mikrotom
dengan ketebalan 2 mikron.
Hasil pemotongan dimasukkan ke air hangat agar
mengembang tempelkan pada kaca objek lalu
beri identitas
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4. Preparat dipanaskan dalam oven 70 derajat selama
10 menit (untuk melelehkan lilin)dinginkan
tata di rak
5. Lakukan proses pewarnaan dengan alat Veristein
dengan total waktu 2,5 jam yang masing-masing
terdiri dari:
- Xylol 1, 2,3 masing-masing 10 menit
fungsi untuk melarutkan lilin
- Etanol 3 menit
- Alkohol 96% 3 menit
- Alkohol 80% 3 menit
- Alkohol 70% 3 menit
- Alkohol 50% 3 menit
C
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- Aqua merupakan pelarut hematoksilin
fungsi menyamakan pH dengan hematoksilin
- Hematoksilin 15 menit (untuk mewarnai
inti)
- HCL1 menit. Fungsi agar pewarnaan tidak
terlalu biru
- Bruing 10 menit fungsi untuk lebih
memperjelas warna inti
- Aqua 3 menit
- Alkohol 50%3 menit
- Alkohol 70% 3 menit f:merupakan
pelarut eosin
- Eosin 3 menit (untuk pewarnaan
sitoplasma)
- Alkohol 70% 3 menit
- Alkohol 96%3 menit
- Etanol 3 menit (alkohol bertingkat untuk
dehidrasi lagi)
- Xylol 1 10 menit untuk clearing
- Xylol 2 10 menit
- Xylol 3 10 menit
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6. Preparat yang sudah diwarnai ditutup dengan deck
glass yang sebelumnya ditetesi dengan Ez Mount
dengan tujuan untuk merekatkan deck glass dan
sebagai pengawet (bisa disimpan sampai 10 tahun)
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Lampiran 5. Lembar Pengumpulan Data
No. sampel :
1. IDENTITAS
- Nama / umur :
- Alamat :
- No. register :
- Riwayat obstetri :
- Diagnosis :
2. HASIL PEMERIKSAAN PATOLOGI ANATOMI
Hasil
pengukuran
Arteri
umbilikalis
1
Arteri
umbilikalis
2
Rata-
rata
keliling
lumen
(
)
Rata-rata
diameter
lumen
arteri
umbilikalis
(
)
{ (
)
}
Rata-rata
luas lumen
arteri
umbilikalis
Keliling
lumen
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Lampiran 6. Hasil pemeriksaan
DIAGNOSIS UMUR UMURKEHA
MILAN
CARAPERS
ALINAN PARITAS
KELILING
LUMEN
DIAMETER
LUMEN
ARTERI
UMBILIKAL
IS
LUAS
LUMEN
ARTERI
UMBILIKAL
IS
N1 25 39 1 2 2054.950 654 335757.06
N2 27 40 1 1 1887.475 601 283542.785
N3 33 38 1 2 2825.750 899 634437.785
N4 39 38 3 2 2442.980 778 475147.94
N5 25 39 2 1 3156.200 1005 792869.625
N6 35 38 1 2 6494.705 2068 3357149.84
N7 27 39 1 1 2218.795 706 391272.26
N8 26 41 3 1 2248.055 715 401311.625
N9 36 38 3 2 2455.335 781 478819.385
N10 20 38 3 1 2944.765 937 689205.665
N11 28 38 1 2 2196.735 699 383551.785
N12 21 42 2 1 2011.385 640 321536
N13 23 38 2 2 2014.050 641 322541.585
N14 24 40 2 1 2689.205 856 575197.76
N15 32 38 3 1 2782.175 886 616221.86
N16 32 38 1 1 3101.930 987 764722.665
PE1 39 38 1 2 12289.095 3913 12019581.67
PE2 35 38 1 2 1450.835 462 167553.54
PE3 36 38 2 2 2204.425 702 386851.14
PE4 40 39 3 2 2339.480 745 435694.625
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Lampiran 6. Hasil pemeriksaan (lanjutan)
DIAGNOSIS UMUR UMURKEHA
MILAN
CARAPERS
ALINAN PARITAS
KELILING
LUMEN
DIAMETER
LUMEN
ARTERI
UMBILIKAL
IS
LUAS
LUMEN
ARTERI
UMBILIKAL
IS
PE5 20 39 1 1 5461.575 1739 2373934.985
PE6 39 29 2 2 4240.245 1350 1430662.5
PE7 30 36 1 2 3452.845 1099 948123.785
PE8 26 37 3 2 3326.250 1059 880362.585
PE9 18 39 1 1 3956.085 1259 1244288.585
PE10 36 38 1 2 3709.470 1181 1094887.385
PE11 34 38 2 2 3498.700 1114 974181.86
PE12 35 39 3 2 7693.960 2450 4711962.5
PE13 34 39 1 2 1952.665 621 302728.185
PE14 38 36 3 2 3273.020 1042 852324.74
PE15 31 36 3 2 2548.495 811 516310.985
PE16 37 35 3 1 3476.830 1107 961977.465
DIAGNOSIS RATA-RATA DIAMETER LUMEN
ARTERI UMBILIKALIS
NORMOTENSI 865.8125
PREEKLAMPSIA BERAT 1290.875
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Lampiran 7. Hasil analisis
Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
USIA .135 32 .143 .941 32 .079
UMUR_KEHAMILAN .254 32 .000 .852 32 .000
CARA PERSALINAN .282 32 .000 .753 32 .000
PARITAS .402 32 .000 .615 32 .000
a. Lilliefors Significance Correction
Independent Samples Test
t-test for Equality of Means
t df Sig. (2-
tailed)
Mean
Difference
Std. Error
Difference
95% Confidence Interval of the
Difference
Lower Upper
USIA Equal variances assumed 2.176 30 .038 4.688 2.154 .289 9.086
Equal variances not assumed 2.176 29.248 .038 4.688 2.154 .284 9.091
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Lampiran 7. Hasil analisis (lanjutan)
Test Statisticsa
UMUR_KEHAM
ILAN
CARA
PERSALINAN
PARITAS
Mann-Whitney U 85.500 123.500 80.000
Wilcoxon W 221.500 259.500 216.000
Z -1.697 -.182 -2.156
Asymp. Sig. (2-tailed) .090 .855 .031
Exact Sig. [2*(1-tailed Sig.)] .110b .867
b .073
b
a. Grouping Variable: DIAGNOSIS
b. Not corrected for ties.
Report
DIAGNOSIS USIA UMUR_KEHAM
ILAN
CARA
PERSALINAN
PARITAS
Preeklampsia Berat
Mean 33.00 38.06 1.94 1.81
Std. Deviation 6.563 .854 .929 .403
Median 35.00 38.00 2.00 2.00
Minimum 18 37 Persalinan
Spontan
Primipara
Maximum 40 39 Sectio Caesaria Multipara
Sum 528 609 31 29
Normotensi
Mean 28.31 38.88 1.88 1.44
Std. Deviation 5.582 1.258 .885 .512
Median 27.00 38.00 2.00 1.00
Minimum 20 38 Persalinan
Spontan
Primipara
Maximum 39 42 Sectio Caesaria Multipara
Sum 453 622 30 23
Total
Mean 30.66 38.47 1.91 1.63
Std. Deviation 6.449 1.135 .893 .492
Median 32.00 38.00 2.00 2.00
Minimum 18 37 Persalinan
Spontan
Primipara
Maximum 40 42 Sectio Caesaria Multipara
Sum 981 1231 61 52
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Test Statisticsa
diameter_makro
s
Mann-Whitney U 48.500
Wilcoxon W 184.500
Z -3.002
Asymp. Sig. (2-tailed) .003
Exact Sig. [2*(1-tailed Sig.)] .002b
a. Grouping Variable: diagnosis
b. Not corrected for ties.
Report
DIAMETER LUMEN ARTERI UBILIKALIS
DIAGNOSIS Mean Std. Deviation Median Minimum Maximum Sum
Preeklampsia Berat 1290.8750 843.20522 1103.0000 462.00 3913.00 20654.00
Normotensi 865.8125 345.78649 779.5000 601.00 2068.00 13853.00
Total 1078.3437 669.71046 892.5000 462.00 3913.00 34507.00
Lampiran 7. Hasil analisis (lanjutan)
Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
DIAMETER LUMEN ARTERI UBILIKALIS .260 32 .000 .670 32 .000
a. Lilliefors Significance Correction
Test Statisticsa
DIAMETER
LUMEN
ARTERI
UBILIKALIS
Mann-Whitney U 67.000
Wilcoxon W 203.000
Z -2.299
Asymp. Sig. (2-tailed) .022
Exact Sig. [2*(1-tailed Sig.)] .021b
a. Grouping Variable: DIAGNOSIS
b. Not corrected for ties.
Tests of Normality
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
diameter_makros .164 32 .029 .918 32 .019
a. Lilliefors Significance Correction
83
Lampiran 8. Dokumentasi
83
Lampiran 9. Biodata Mahasiswa
Identitas
Nama : Aulia Rizqi Mulyani
NIM : 22010111130128
Tempat/tanggal lahir : Tegal/ 6 Maret 1993
Jenis kelamin : Perempuan
Alamat : Cluster Edelweis no 26 Permata Tembalang Semarang
Nomor Telpon : -
Nomor HP : 081225919130
e-mail : [email protected]
Riwayat Pendidikan Formal
1. TK : TK Perwanida 2 Slawi Lulus tahun : 1999
2. SD : SDN Mangkukusuman 7 Tegal Lulus tahun : 2005
3. SMP : Semesta Bilingual Boarding School Lulus tahun : 2008
4. SMA : Semesta Bilingual Boarding School Lulus tahun : 2011
5. FK/UNDIP : Masuk tahun : 2011
Keanggotaan Organisasi
1. Staf Hubungan Luar (HUBLU) HIMA KU UNDIP Tahun 2011 s/d 2014
2. Bendahara AMSA FK UNDIP Tahun 2012 s/d 2013
3. Anggota volunteer Group Work Camp Tahun 2011 s/d 2014
4. Ambassador of Publich Health Distric 4 AMSA Indonesia Tahun 2013/2014
Pengalaman penelitian
-
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