51009904 Thuc Hanh Ky Thuat Duong Chuan 0454
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Transcript of 51009904 Thuc Hanh Ky Thuat Duong Chuan 0454
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Nhm thc hin: Nhm 01GVHD: Th.s Trn Nguyn An Sa
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Ni dungTng quan k thut ng chunng dng xc nh hm lng Fe(II) v tng Fe Nguyn tcCc cht gy nhiuHo chtDng c - thit bCch tin hnh
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K thut ng chun
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u im- Vi mt ng chun cho php phn tch hng lot mu nn nhanh v hiu qu kinh t.2.Nhc imCn c my, my cng chnh xc th kt qu cng tin cy.S hp thu nh sng ca dung dch phi tun theo nh lut Bughe- Lambe- Beer.Khng loi c nh hng ca nn mu.u v nhc im
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ng dng xc nh hm lng Fe(II) v tng Fe
St l nguyn t vi lng cn thit cho c th. V th, st vi hmlng 0,3 mg/l l mc cho php i vi nc sinh hot. Vt qu giihn trn, st c th gy ra nhng tr ngi:Mi tanh c trng ca st, lm nc c mu to n tng khng tt cho ngi s dng. Nc c st khng th dng cho mt s nghnh cng nghip nh: giy, si, dt, thc phm, dc phm Kt ta st lng ng thu hp dn tit din hu dng ca ng dn trong mng li phn phi nc.
Do cc l do trn, vic xc nh hm lng st trong nc c nghaquan trng trong i sng cng nh trong sn xut cng nghip.
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Fe2+ to phc bn c mu cam vi 1,10 phenanthroline.Phc [Fe(Phen)3]2+ bn c max 508nm, cng mu khng thay i trong khong pH t 2 9.
Tng hm lng St: dng hidroxylamine kh Fe3+ v Fe2+.Hm lng Fe3+ = tng hm lng Fe - hm lng Fe2+
Nguyn tc
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Nhng cht oxi ha mnh: CN-, NO2-, PO43- Zinc vi hm lng ln hn st 10 lnCopper ln hn 5ppmNiken ln hn 2ppmBitmut,thy ngn, molybdate, bc kt ta vi phenanthroline (thm mt lng tha phenanthroline)Cht hu c (un si mu nhiu gi vi HCl 1:1trong cc s, khi mu cn, t nh, phn tro cn li ha tan bng axit).Cc cht gy cn nhiu
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Ho cht
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4. Dung dch Fe3+ 15ppm
5. Dung dch NH2OH 10% pha trong nc.6. m axetat pH = 5.7. Dung dch 1,10 phenanthroline 0.5% pha trong nc.
Ho cht
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Bnh nh mc 1L01Pipet vch 10ml 01Cuvet 01My pH k01Cn phn tch01Bp cao su01Beaker 250ml03Beaker 100ml02My khuy t01a thy tinh01Pipet bu 5ml02C t01My so mu01
Dng c - thit bMy quang ph Genesye 10UV scanning
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Cch tin hnhQut ph v xy dng ng chun.Hiu sut thu hi ca phng php.Kim tra mu th.Vn hnh thit b.
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Qut ph v xy dng ng chun
Bnh s123456VFe 50ppm (ml)01.02.03.04.05.0Hydroxylamin (ml)2.0Dung dch m pH = 5 (ml)10.01,10- phenanthroline2.0nh mc bng nc ct n vch 50ml
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Qut ph v xy dng ng chun
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Hiu sut thu hi ca phng php
Bnh s1234567V_dd B101010V_ dd C101010Hydroxylamin2.00.02.0 yn 10 phtDung dch m pH = 5 (ml)10.01,10- phenanthroline (ml)2.0nh mc bng nc ct n vch 50ml
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Kim tra mu
Bnh s1234567V_ddA (ml)101010101010Hydroxylamin (ml)2.00.02.0 yn 10 phtDung dch m pH = 5 (ml)10.01,10- phenanthroline (ml)2.0nh mc bng nc ct n vch 50ml
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Vn hnh my
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Trng hp my khng kt ni vi phn mm Visionlite
Nhn cng tc ngun ON/OFF trn my, ch n nh.Nhn phm Test mn hnh hin th:
- Advanced A; %T; C- Kinetics- Standard curve- Scanning- Absorbance Ratio- 3-point Net- Absorbance Difference- Mulatiwavelength- Performance ValidationVn hnh my
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Nu qut bc sng, nhn phm hoc chn Scanning; sau Enter. Chn khong bc sng cn qut v chn lnh qut bc sng.- Chy nn bng cch qut vi mu trng.- Chy mu bng cch chn mt mu bt k qut.Nu o mu ring bit nhn phm hoc chn ch o Advanced A ; %T; C.Nu xy dng ng chun ta nhn phm hoc chn Standard curve.Vn hnh my
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2. Trng hp my o quang kt ni vi phn mm VisionliteNhn cng tc ngun ON/OFF trn my, ch n nh.Khi ng my vi tnh, m phn mm Visionlite
Nu qut bc sng ta chn ch Scan v thc hin cc bc sau:
Vn hnh my
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Phn mm gm 4 module: Scan: Ghi nhn ph. Rate:Fixed: Ghi nhn s hp th ca mu ti mt im ca mt bc sng.Quant: nh lng theo nh lut Lambert Beer.
Scan.mscRate.mraFixed.mfxQuant.mqa
Vn hnh my
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Scan
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Rate
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Fixed
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Quant
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Vn hnh my Nhp cc thng s ca ch qut
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- Qut mu trng bng nc ct hoc bnh Blank, ta cho mutrng vo my quang, nhn Baseline hoc Measure samples v sau check vo mc Measure blank v nhnMeasure qut mu trng.
- Sau khi qut mu trng (my bo 100%), ta t mu tht vov nhn Measure qut mu tht, v lu file vi ui msc.Nu xy dng ng chun, ta chn ch Quant, v thc hin cc bc sau:
Vn hnh my
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Vn hnh my - Nhp cc thng s xy dng ng chun
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Sau khi nhp cc thng s xy dng ng chun, ta nhn Measure standards v sau check vo mc Measure blank v nhn Measure o mu trng. Sau khi o mu trng (my bo 100%) , ta ln lt t cc chun t thp n cao v nhn Measure o chun, lu file vi ui mqa. o mu, ta m li file ng chun lu, t mu trng v nhn Measure sample v sau check vo mc Measure blank v nhn Measure o mu trng. Sau khi o mu trng (my bo 100%), ta ln lt t mu tht, nhp thng tin mu, nhn Measure o mu tht.Vn hnh my
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Kt lunNm r kin thc v k thut ng chun.ng dng xc nh hm lng Fe2+ v Fe tng.Cch kho st bc sng cc i, xy dng ng chun.Vn hnh my o quang ph Genesye 10UV scanning v phn mm Visionlite.
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Ti liu tham khoGio trnh thc hnh phn tch ha l.Trn T Hiu(2003), Phn tch trc quang ( Ph hp th UV Vis), NXB i hc quc gia H Ni, H Ni.Http://www.google.com.vn/searchHttp://www. Bioblock.com