超高速バイオアセンブラ

 NewsletterHyper  Bio  Assembler   for  3D  Cel lu lar   Innovat ion

No.4

Contents研究組織・研究項目...2A01班  金子  真  研究紹介...3A02班  福田  敏男  研究紹介...4A03班  鈴木  治  研究紹介...5研究業績紹介...6-14第1回若手研究者第1回若手研究者

     シンポジウム報告...15Information....................16

2013年3月号

http://bio-asm.jp

科学研究費補助金　「新学術領域研究」　平成23年度発足領域略称名：バイオアセンブラ

設定期間：平成23年度～平成27年度

領域代表：大阪大学大学院基礎工学研究科　教授  新井健生

A03

A

02

2

A01

A02 3

3

3

MEMS 3

4

3

A03 3

3

Organ-Explant-Chip

in vitro

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3

[ ]

A02 H24

1/3

(a) (b)

A01 A01-2

4

EVE(Endovascular Evaluator)

3

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(1)

(E-SEM)

(2) 3

3

(3)3

A02 A02-02

5

[ ]

HA

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Suzuki O, Anada T et al. Biomaterials 2006; Tissue Eng Part A

2008; ibid 2009: Cell Tissue Res 2013; J Tissue Eng Regen Med 2013

A01

30

3E1-3 2012

Anada T, Suzuki O et al. Biomaterials 2012

A03 A03-02

Bar=500μm

6

A01

1) Kawahara T, Sugita M, Hagiwara M, Arai F, Kawano H,

Shihira-Ishikawa I, Miyawaki A. On-chip microrobot for investigation of stimulant property of aquatic microorganisms. Lab Chip 2012; DOI: 10.1039/C2LC41190C.

2) Masuda T, Takei N, Nakano T, Anada T, Suzuki O, Arai F, A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomed Microdevices, 2012; 14: 1085-1093. DOI 10.1007/s10544-012-9713-0, 2012.

3) Hagiwara M, Kawahara T, Iijima T, Arai F. High speed magnetic microrobot actuation in a microfluidic chip by fine V-groove surface. IEEE T Robot 2012; DOI: 10.1109/TRO.2012.2228310.

4) . V

. 2012; 30: 727-34. 5) Hagiwara M, Kawahara T, Arai F., Local streamline

generation by mechanical oscillation in a microfluidic chip for noncontact cell manipulations, Appl Phys Lett, 2012; 101: 074102-1-5.

6) , , “”,

, 2012; 30: 411–419, 2012. 7) Kawahara T, Ohashi S, Hagiwara M, Yamanishi Y, Arai F.

Air-flow-based single-cell dispensing system. Advanced Robotics 2012; 26: 291-306.

8) Feng L, Kawahara T, Yamanishi Y, Hagiwara M, Kosuge K, Arai F. On-chip capacitance sensor based size controllable droplet generation. Journal of Robotics and Mechatronics 2012; 24: 133-40.

9) Onda K, Arai F, “Multi-beam bilateral teleoperation of holographic optical tweezers accelerated by general-purpose GPU”, Optics Express, 2012; 20: 3642-3653.

20

1. Best Paper Award, 2012 11 2012 International

Symposium on Micro-NanoMechatronics and Human Sciences “On-chip Cell Loading Using untethered Nano-pipette Robot”. Ichikawa A, Sakuma S, Arai F.

2. Best Paper Award, 2012 11 2012 International Symposium on Micro-NanoMechatronics and Human Sciences “Virus Chromatography; On-chip Diagnosis of Virus Infection from Human Body Fluid”. Niimi M, Masuda T, Kaihatsu K, Kato N, Arai F.

3. IEEE Robotics and Automation Society Japan Chapter Young Award, 2012 10 The 2012 IEEE/RSJ Int. Conf. on Intelligent Robots and Systems “Organ-explanted Bionic Simulator (OBiS) : Concurrent Microcardiovascular Anastomosis of Chick Embryo”. Owaki H.

4. ROBOMEC , 2012 5

2011 “―

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7. Best Conference Paper Award, 2012 5 2012 IEEE

International Conference on Robotics and Automation“High speed microrobot actuation in a microfluidic chip by levitated structure with riblet surface”. Hagiwara M, Kawahara T, Iijima T, Yamanishi Y, Arai F.

8

2012 11 16

1) Tsai C, Kao I, Higashimori M, and Kaneko M. Modeling, Sensing and Interpretation of Viscoelastic Contact Interface. Journal of Advanced Robotics, 2012; 11-12, 1393-1418.

2) .

, 2012; 6: 655-661. 3) Imin K.

. 4)

.

1) Fukui W, Kaneko M, Sakuma S, Kawahara T, and Arai F. -cell fatigue test. Proc. of the IEEE Int. Conf. on Robotics and Automation 2012(ICRA2012), May, 2012; 4600-4605.

2) Kimura Y, Izumi I, Kaneko M, Ikuno Y, Miki A, Jo Y, Kiuchi Y. Deformation of macula area under compulsory increase of eye pressure. Proc. of the IEEE Int. Conf. on Mechatronics and Automation (ICMA2012); August, 2012; 980-985.

3) Tsai C, Kaneko M, Sakuma S and Arai F. Phase decomposition of a cell passing through  a  μ-channel - a method for improving the evaluation of cell stiffness. Proc. of the IEEE Int. Conf. on Mechatronics and Automation(ICMA2012), August, 2012, 138-143.

4) Kimura Y, Izumi I, Kaneko M, Ikuno Y, Miki A, Jo Y, Kiuchi Y. Deformation of yellow spot area by compulsory increase of eye pressure. Proc. of the 34th Annual International Conference of the IEEE Engineering in Medicine & Biology Society(EMBC2012), August, 2012; 1474-1477.

5) Tsai C, Kaneko M and Arai F. Evaluation of cell impedance using  a  μ-channel. Proc. of the 34th Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC12), August, 2012; 5518-5521.

6) Tsai C, Kaneko M and Arai F. Enhanced cell stiffness evaluation by two-phase decomposition. Proceeding of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS12), October, 2012; 1009-1011.

7) Tsai C, Kaneko M, Sakuma S. and Arai F. Biomechanical properties of red blood cell through the motion inside a micro-channel. Proc. of the 23rd 2012 international symposium on Micro-NanoMechatronics and Human Science(MHS2012), November, 2012; 390-392.

8) Fukui W, Kaneko M, Sakuma S, and Arai F. Cell fatigue test.

7

Proc. of the 26th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2013), January, 2013; 1025-1028.

9) Tsai C, Kaneko M, Sakuma S and Arai F. Observability of cell stiffness in micro-channel method. Proc. of the IEEE Int. Conf. on Robotics and Automation(ICRA2013), May, 2013; accepted.

1) IEEE Robotics and Automation Society Japan Chapter Young Award.

Fukui W, Kaneko M, Sakuma S, Kawahara T, and Arai F. -cell fatigue test. Proc. of the IEEE Int. Conf. on Robotics and Automation 2012(ICRA2012), May, 2012; 4600-4605.

2) Best Paper Award in the IEEE Int. Conf. on Mechatronics and Automation (ICMA12).

Tsai C, Kaneko M, Sakuma S and Arai F. Phase decomposition of a cell passing through  a  μ-channel - a method for improving the evaluation of cell stiffness. Proc. of the IEEE Int. Conf. on Mechatronics and Automation(ICMA2012), August, 2012, 138-143.

3) , The second prize of the paper competition of JpCOMPEmbs12.

Kimura Y, Izumi I, Kaneko M, Ikuno Y, Miki A, Jo Y, Kiuchi Y. Deformation of yellow spot area by compulsory increase of eye pressure. Proc. of the 34th Annual International Conference of the IEEE Engineering in Medicine & Biology Society(EMBC2012), August, 2012; 1474-1477.

1. Toshinobu Nishimura, Shin Kaneko, Ai Kawana-Tachikawa, Yoko Tajima, Haruo Gotoh, Dayong Zhu, Kaori Nakayama, Shoichi Iriguchi, Yasushi Uemura, Takafumi Shimizu, Naoya Takayama, Daisuke Yamada, Ken Nishimura, Manami Ohtaka, Nobukazu Watanabe, Satoshi Takahashi, Aikichi Iwamoto, Haruhiko Koseki, Mahito Nakanishi, Koji Eto and Hiromitsu Nakauchi. Generation of rejuvenated antigen-specific T cells by pluripotency reprogramming and dedifferentiation. Cell Stem Cell. 12: 114-126m 2013

2. Oikawa T, Kamiya A, Zeniya M, Chikada H, Hyuck AD, Yamazaki Y, Wauthier E, Tajiri H, Miller LD, Wang XW, Reid LM, Nakauchi H. SALL4, a stem cell biomarker in liver cancers. Hepatology. 2012 Nov 23. doi: 10.1002/hep.26159.

3. Nakajima-Takagi Y, Osawa M, Oshima M, Takagi H, Miyagi S, Endoh M, Endo TA, Takayama N, Eto K, Toyoda T, Koseki H, Nakauchi H, Iwama A. Role of SOX17 in hematopoietic development from human embryonic stem cells. Blood. 2012 Nov 20.

4. Lin HT, Otsu M, Nakauchi H. Stem cell therapy: an exercise in patience and prudence. Philos Trans R Soc Lond B Biol Sci. 2013 Jan 5;368(1609):20110334. doi: 10.1098/rstb.2011.0334.

5. Matsuno N, Yamamoto H, Watanabe N, Uchida N, Ota H, Nishida A, Ikebe T, Ishiwata K, Nakano N, Tsuji M, Asano-Mori Y, Izutsu K, Masuoka K, Wake A, Yoneyama A, Nakauchi H, Taniguchi S. Rapid T-cell chimerism switch and memory T-cell expansion are associated with pre-engraftment immune reaction early after cord blood transplantation. Br J Haematol. 2012 Nov 1. doi: 10.1111/bjh.12097.

6. Kamiya A, Nakauchi H. Enrichment and clonal culture of hepatic stem/progenitor cells during mouse liver development. Methods Mol Biol. 2013;945:273-86. doi: 10.1007/978-1-62703-125-7_16.

7. Hirabayashi M, Tamura C, Sanbo M, Kato-Itoh M, Kobayashi

T, Nakauchi H, Hochi S. A retrospective analysis of germline competence in rat embryonic stem cell lines. Transgenic Res. 2012 Aug 9. [Epub ahead of print]

8. Kajiwara M, Aoi T, Okita K, Takahashi R, Inoue H, Takayama N, Endo H, Eto K, Toguchida J, Uemoto S, Yamanaka S. Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12538-43. doi: 10.1073/pnas.1209979109. (Epub 2012 Jul 16.) Erratum in: Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14716.

9. Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H. Development of an All-in-One Inducible Lentiviral Vector for Gene Specific Analysis of Reprogramming. PLoS One. 2012;7(7):e41007. Epub 2012 Jul 18.

10. Nakamura S, Oshima M, Yuan J, Saraya A, Miyagi S, Konuma T, Yamazaki S, Osawa M, Nakauchi H, Koseki H, Iwama A. Bmi1 confers resistance to oxidative stress on hematopoietic stem cells. PLoS One. 2012;7(5):e36209. Epub 2012 May 11.

11. Kumano K, Arai S, Hosoi M, Taoka K, Takayama N, Otsu M, Nagae G, Ueda K, Nakazaki K, Kamikubo Y, Eto K, Aburatani H, Nakauchi H, Kurokawa M. Generation of induced pluripotent stem cells from primary chronic myelogenous leukemia patient samples. Blood. 2012 Jun 28;119(26):6234-42. Epub 2012 May 16.

12. Takayama N, Eto K. Pluripotent stem cells reveal the developmental biology of human megakaryocytes and provide a source of platelets for clinical application. Cell Mol Life Sci. 19(26):6234-42, 2012. doi: 10.1007/s00018-012-0995-4.2012 Apr 24.

18

2012 9M&M2012 “

” ○ .

1)

52, 230-233 (2012) . 2)

33, 461-466 (2012) . 3)

(2013) . 4) Y. Mizutani, Z. Ishikura, M.-H. Choi, S.-J. Cho, T. Okajima,

“Nanoscale cell membrane fluctuations measured by scanning ion conductance microscopy”, Biophysical Society 57th Annual Meeting (2013).

5) Y. Mizutani, M.-H. Choi, S.-J. Cho2, and T. Okajima, “Quantitative Measurement of Cell Membrane Fluctuations by Scanning Ion Conductance Microscopy” 20th International Colloquium on Scanning Probe Microscopy (2012).

6) ” ”30

2012 . 7) T. Okajima, “Exploring mechanical properties of live cells

on microfabricated substrates by atomic force microscopy”,

8

The 7th Nagoya University-UCLA International Symposium (2012).

8) T. Okajima, “Fluctuations of single cells measured by atomic force microscopy and scanning ion conductance microscopy”, 3rd Kanazawa Bio-AFM Workshop (2012)

9) R. Mizuto, Y. Sakai, R. Takahashi, T. Okajima, “Cell cycle dependence of cell mechanics investigated by traction force microscopy” 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012), (2012).

10) ”50

2012 11)

502012

12) 50

2012

(1) Hao Gao, Qingyi Gu, Takeshi Takaki, and Idaku Ishii : A Self-Projected Light-Section Method for Fast Three-Dimensional Shape Inspection, International Journal of Optomechatronics, Vol.6, No.4, pp.289-303 (2012)

(2) Qingyi Gu, Takeshi Takaki, and Idaku Ishii : Fast FPGA-Based Multi-Object Feature Extraction, IEEE Transactions on Circuits and Systems for Video Technology, doi : 10.1109/TCSVT.2012.2202195 (early access articles) (2012)

(3) Lei Chen, Hua Yang, Takeshi Takaki, and Idaku Ishii : Real-Time Optical Flow Estimation Using Multiple Frame-Straddling Intervals, Journal of Robotics and Mechatronics, Vol.24, No.4, pp.686-698 (2012)

(4) , , : ,

(C ), Vol.78, No.788, pp.1143-1153 (2012) (5) Idaku Ishii, Tomoki Ichida, Qingyi Gu, and Takeshi Takaki :

500-fps Face Tracking System, Journal of Real-Time Image Processing, doi: 10.1007/s11554-012-0255-8 (online first) (2012)

(6) Lei Chen, Takeshi Takaki, and Idaku Ishii : Accuracy of Gradient-Based Optical Flow Estimation in High-Frame-Rate Video Analysis, IEICE Transactions on Information and Systems Vol.E95-D, No.4,

1. 24 Best Automation Award Finalist, IEEE Int. Conf. on Robotics and Automation

"A Real-Time Micro-PIV System Using Frame-Straddling High-Speed Vision", Motofumi Kobatake, Takeshi Takaki, and Idaku Ishii

2. 24 PIV

( 3. 24 (

)

4. 24 ROBOMEC

1) Koide, M., Yasukawa, T., Horiguchi, Y., Nagamine, K., Shiku, H., Matsue, T., Itayama, T. Microfluidic Devices for Electrochemical Measurement of Photosynthetic Activity of Cyanobacteria Microcystis Cells. Anal. Sci. 2012; 28: 69-72.

2) Yasukawa, T., Yoshimura, Y., Mizutani F., Microsensors for Glucose in High Concentration Range by Controlling Oxygen Concentration. Electrochemistry 2012; 80: 15-17.

3) Hirano, Y., Yasukawa, T., Mase, Y., Oyamatsu, D., Shiku, H., Mizutani, F., Matsue, T., Imaging of Enzyme Reactions Captured via Immuno-recognition by Scanning Electrochemical Microscopy with Distance Control System. Electrochemistry 2012; 80: 30-32.

4) Yasukawa, T., Yamada, J., Shiku, H., Mizutani, F., Matsue, T., Negative Dielectrophoretic particle positioning in a fluidic flow. Intelligent Automation and Soft Computing 2012; 18:, 201-211.

5) Yasukawa, T., Yoshimoto, Y., Goto, T., Mizutani, F., Highly-sensitive electrochemical immunosensing method based on dual amplification systems. Biosensors and Bioelectronics 2012; 37: 19-23.

6) Yamamoto, M., Yasukawa, T., Suzuki, M., Kosuge, S., Shiku, H., Matsue, T., Mizutani, F., Patterning with Particles Using Three-Dimensional Interdigitated Array Electrodes with Negative Dielectrophoresis and Its Application to Simple Immunosensing. Electrochim. Acta 2012; 82: 35-42.

7) Kiba, Y., Otani, Y., Yasukawa, T., Mizutani, F., Electrochemical detection of redox species flowing in a nitrocellulose membrane and application to quantitative immunochromatography. Electrochimica Acta 2012; 81: 14– 19.

8) Yasukawa, T., Hatanaka, H., Mizutani, F., Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis. Anal. Chem. 2012; 84: 8830-8836.

10

1. 25

24 5 18 2.

25 1 25

A02 3

1) Ebubekir Avci, Chanh-Nghiem Nguyen, Kenichi Ohara,

Yasushi Mae, Tatsuo Arai, “Analysis and Suppression of Residual Vibration in Microhand for High-Speed Single-Cell Manipulation”, International Journal of Mechatronics and Automation, Vol. 3, No. 2, 2013 (accepted).

2) Ebubekir Avci, Kenichi Ohara, Tomohito Takubo, Yasushi Mae, Tatsuo Arai: "Development of Multi-Scalable Microhand System with Precise Motion Ability", Journal of Robotics and Mechatronics, Vol. 25, No. 1, 2013 (accepted).

3) Chanh-Nghiem Nguyen, Kenichi Ohara, Yasushi Mae, and Tatsuo Arai: "High-Speed Focusing and Tracking of Multisized Microbiological Objects," Journal of Robotics and Mechatronics, Vol. 25, No. 1, 2013 (accepted).

9

4) Kenichi Ohara, Daiki Kawakami, Tomohito Takubo, Yasushi Mae, Tamio Tanikawa, Ayae Honda and Tatsuo Arai, “Dextrous cell diagnosis using two-fingered microhand with micro force sensor”, Journal of Micro Nano Mechatronics, Vol. 7, No. 1~3, pp. 13-20, 2012.

5) Chanh-Nghiem Nguyen, Kenichi Ohara, Ebubekir AVCI, Tomohito Takubo, Yasushi Mae, and Tatsuo Arai, “Real-time Precise 3D Measurement of Micro Transparent Objects Using All-In-Focus Imaging System”, Journal of Micro Nano Mechatronics, Vol. 7, No. 1~3, pp. 21-31, 2012.

10

1) Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda, Nanofork for Single Cells Adhesion Measurement via ESEM-Nanomanipulator System, IEEE Transactions on Nanobioscience, Vol. 11, No. 1, (2012), pp. 70-78.

2) Mohd Ridzuan Ahmad, Masahiro Nakajima, Masaru Kojima, Seiji Kojima, Michio Homma, Toshio Fukuda, "Instantaneous and Quantitative Single Cells Viability Determination Using Dual Nanoprobe Inside ESEM", IEEE Transactions on Nanotechnology, Vol. 11, No. 2, (2012), pp. 298-306.

3) Masaru Kojima, Zhenhai Zhang, Masahiro Nakajima, Toshio Fukuda, "High efficiency motility of bacteria-driven liposome with raft domain binding method", Biomed Microdevices, Vol. 14, Issue 6, (2012), pp 1027-1032.

4) Masahiro Kojima, Keiko Irie, Seiichi Ikeda, Toshio Fukuda, Fumihito Arai, Yuichi Hirose, Makoto Negoro, “The hemodynamic study for growth factor evaluation of rupture cerebral aneurysm followed up for five years”, Journal of Biomedical Science and Engineering, (2012), pp. 884-891.

5) C. Shi, M. Kojima, H. Anzai, C. Tercero, S. Ikeda, M. Ohta, T. Fukuda, F. Arai, M. Negoro, K. Irie. “In-vitro Strain Measurement in Cerebral Aneurysm Models for Cyber-Physical Diagnostic”, International Journal of Medical Robotics and Computer Assisted Surgery, Vol. 8, (2012), pp. 291-199.

6) C. Hu, C. Tercero, S. Ikeda, M. Nakajima, H. Tajima, Y. Shen, T. Fukuda, and F. Arai, “Biodegradable Porous Sheet-like Scaffolds for Soft-Tissue Engineering using a Combined Particulate Leaching of Salt Particles and Magnetic Sugar Particles”, Journal of Bioscience and Bioengineering, Accepted

7) Masahiro Kojima, Keiko Irie, Toshio Fukuda, Fumihito Arai, Yuichi Hirose, Maokoto Negoro, “The Study of Flow Diversion Effects on Aneurysm Using Multiple Enterprise Stents and Two Flow Diverters”, Asian Journal of Neurosurgery, Vol. 8 Issue 3, 2013(Accepted)

8) Masaru Takeuchi, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda, “Fabrication and Appliation of Probe Device using Thermoresponsive Gel for Handling of Micro Objects”, Journal of Robotics Society of Japan Vol. 31, No. 3, 2013. (in Japanese, Accepted)

9) Masaru Takeuchi, Masahiro Nakajima, Masaru Kojima, Toshio Fukuda, “Handling of Micro Objects using Phase Transition of Thermoresponsive Polymer”, Journal of Micro-Bio Robotics, (Accepted, DOI:10.1007/s12213-013-0060-x)

10) Tao Yue, Masahiro Nakajima, Hirotaka Tajima and Toshio Fukuda, “Fabrication of microstructures embedding controllable particles inside dielectrophoretic microfluidic

devices”, International Journal of Advanced Robotic Systems. (Accepted)

1. 2012.9.28 Friendship Award of Liaoning Province , in appreciation of your enthusiastic support and

contribution to the construction of Liaoning Province, Toshio Fukuda

2. 2012.11.7 Best Paper Award in 2012 International Symposium on Micro-Nano Mechatronics and Human Science (MHS2012), “Local Injection Probe of Functional Micro-Nano Gel Tools into Caenorhabditis elegans”, Masahiro Nakajima, Naoya Nakanishi, Naoki Hisamoto, Hirotaka Tajima, Michio Homma, Toshio Fukuda

3. 2012.11.7 Best Poster Award in 2012 International Symposium on Micro-Nano Mechatronics and Human Science (MHS2012), “An In Vitro Blood Vessel Model made of PVA Hydrogel for Vascular Surgery Training”, Seiichi Ikeda and Toshio Fukuda

1) Toyama K, Yamada M, Seki M. Isolation of cell nuclei in microchannels by short-term chemical treatment via two-step carrier medium exchange. Biomed Microdev 2012; 14: 751-7.

2) Yamada M, Utoh R, Ohashi K, Tatsumi K, Yamato M, Okano T, Seki M. Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions. Biomaterials 2012; 33: 8304-15.

3) Sugaya S, Kakegawa S, Fukushima S, Yamada M, Seki M. Micropatterning of hydrogels on locally hydrophilized regions on PDMS by stepwise solution dipping and in situ gelation. Langmuir 2012; 28: 14073-80.

4) Morijiri T, Yamada M, Hikida T, Seki M. Microfluidic counterflow centrifugal elutriation system for sedimentation-based cell separation. Microfluid Nanofluid 2013; in press.

5) Sugaya S, Yamada M, Seki M. Microfluidic synthesis of micrometer-size collagen hydrogel particles for cell manipulation applications. Proc MicroTAS 2012: 944-6.

6) Iwase M, Yamada M, Seki M. Construction of vascular tissues via multilayer cell deposition inside hydrogel microchannels. Proc MicroTAS 2012: 572-4.

10

1. 23

2. 23

1) K. Kuribayashi-Shigetomi, H. Onoe and S. Takeuchi, “Cell Origami: Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force”, PLOS ONE 7(12) e51085, (2012)

1) S. Yoshida, K. Sato, S. Takeuchi, “Sequential Micro-assembly of Three Dimensional Biological

10

Microstructures from Two Dimensional Cell-laden Microplates”, The 1st CIRP Conference on Biomanufacturing, Tokyo, Japan, (To be presented), 4. Mar. (2013)

2) S. Yoshida, S. Takeuchi, “Dissolvable Mobile Microplates for Handling Adherent Cells”, MEMS 2013, Taipei, Taiwan, pp. 959-962, 24. Jan. (2013)

3) T. Teshima, H. Aonuma, H. Onoe, H. Kanuka, and S. Takeuchi, “Vertucak abd Horizontal Confocal Imaging of Single Cells on Magnetically Handleable Microplates” MEMS 2013, Taipei, Taiwan, p. 145, 22. Jan. (2013)

4) Tetsuhiko Teshima, Hiroka Aonuma, Hiroaki Onoe, Hirotaka Kanuka, Shoji Takeuchi, “Magnet-Active Mobile Microplate System to Manipulate Single Adherent Host Cells for the Analysis of Parasite Infection”, Programme and Abstract Book of International Joint Symposium on Single-Cell Analysis, Kyoto, Japan, p.106, 28. Nov.(2012)

5) D. Serien, K. Kuribayashi-Shigetomi, S. Yoshida, and S. Takeuchi, “Control of Self-folding Cell-Laden Microplates By Cytoskeleton Alignment To Fibronectin Patterns”, MicroTAS2012, Okinawa, Japan, pp. 1588-1590, 31. Oct. (2012)

6) Tetsuhiko Teshima, Hiroaki Onoe, Hiroka Aonuma, Kaori Kuribayashi-Shigetomi, Hirotaka Kanuka and Shoji Takeuchi, “Observation of parasite invasion with the micro-fabricated device”, MicroTAS2012, Okinawa, Japan, pp. 103-105, 29. Oct. (2012)

7) S. Yoshida, K. Sato, K. Kuribayashi-Shigetomi, T. Teshima, S. Takeuchi, “Photo-assisted Micro-gluing for Assembling Three-dimensional Microstructures with Living Cells”, MicroTAS2012, Okinawa, Japan, pp.509-511, 29. Oct. (2012)

8) K. Kuribayashi-Shigetomi, H. Onoe and S. Takeuchi, “Self-Folding of Microplates for 3D Cell-Laden Microstructures”, IROS, Algarve, Portugal 7-12. Oct. (2012).

9) , , , “3 ”, , M&M

2012 , , pOS1102, 21-24. Sep. (2012).

10) , , , “3 ”,

, Dynamics and Design Conference, , p173, 18-21. Sep. (2012).

11) , , , , “”

22 , , 12. Sep. (2012) 12) Tetsuhiko Teshima, Hiroka Aonuma, Hiroaki Onoe,

Hirotaka Kanuka, Shoji Takeuchi, “Parylene Micro-flapping System for the Observation of Parasite Infection into Mammalian Cells”, RSC Tokyo International Conference - Micro/Nano Technologies for Analysis of Human Health and Diagnostics, Chiba, Japan, 6. Sep. (2012)

13) Tetsuhiko Teshima, Hiroaki Onoe, Kaori Kuribayashi-Shigetomi, Hiroka Aonuma, Hirotaka Kanuka, Shoji Takeuchi, “Observation of parasite invasion with the micro-fabricated device”, 50 ,

, S36, 15. Aug. (2012) 14) , “MEMS 3

”, 1, , p8, 4. July (2012).

15) K. Kuribayashi-Shigetomi, H. Onoe and S. Takeuchi, “Self-folding 3D Cell Origami for 3D Cell Structures”, ICRA, Minnesota, USA,14-18. May (2012).

1) , Best Presentation Award, “A Parylene

micro-fabricated device toward the analysis of parasite infection into adherent host cells”, Institute of Industrial Science, The Unversity of Tokyo, 9. Sep. (2012)

2) , a Students Poster Award, “Magnet-Active Mobile Microplate System to Manipulate Single Adherent Host Cells for the Analysis of Parasite Infection”, The Single Cell Surveyor Society, 28. Nov. (2012)

2013 1 17 33

2012 12 23 15

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2012 12 13 4

Newton 2013 3 p11

Thomson Reuters 2013 1 1 Scientists use cell to fold

origami NewScientist 2012 12 9 Micro-origami uses cells to

fold itself ( 2012 12 13 4 ) ( 2012 12 13 14 )

11

( 2012 12 23 15 ) ( 2013 1 17 33 )

1. Kazuhiro Hiyama, Hirofumi Matsui, Masato Tamura, Osamu Shimokawa, Mariko Hiyama, Tsuyoshi Kaneko, Yumiko Nagano, Ichinosuke Hyodo, Junko Tanaka, Yoshihiro Miwa, Tetsuo Ogawa, Takeo Nakanishi and Ikumi Tamai. Cancer cells uptake porphyrins via heme carrier protein 1, Journal of Porphyrins and Phthalocyanines, 2012; 16: 1-8

2. Nagano Y, Matsui H, Tamura M, Shimokawa O, Nakamura Y, Kaneko T, Hyodo I. NSAIDs and acidic environment induce gastric mucosal cellular mitochondrial dysfunction. Digestion. 2012;85(2):131-5.

3. Nagano Y, Matsui H, Shimokawa O, Tamura M, Rai K, Nakamura Y, Kaneko T, Indo HP, Majima HJ, Hyodo I. Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID)-induced lipid peroxidation by the MnSOD overexpression in gastrointestinal epithelial cells. J Physiol Pharmacol. 2012 April; 63 (2):137-42

4. Nagano Y, Matsui H, Shimokawa O, Nakamura Y, Tamura M, Rai K, Kaneko T, Hyodo I. Bishophonate-induced gastrointestinal mucosal injury is mediated by mitochondrial superoxide production and lipid peroxidation. J Clin Biochem Nutr.2012; 51(3);196-203

5. Kim BJ, Kim SY, Lee S, Jeon JH, Matsui H, Kwon YK, Kim SJ, So I. The role of transient receptor potential channel blockers in human gastric cancer cell viability. Can J Physiol Pharmacol. 2012 Feb; 90(2):175-86.

6. Okada H, Naito Y, Takagi T, Takaoka M, Oya-Ito T, Fukumoto K, Uchiyama K, Handa O, Kokura S, Nagano Y,

Matsui H, Kato Y, Osawa T, Yoshikawa T. Detection of N-(hexanoyl)lysine in the tropomyosin 1 protein in N-methyl-N'-nitro-N-nitrosoguanidine-induced rat gastric cancer cells. J Clin Biochem Nutr. 2012 Jan; 50(1):47-52.

7. Hiroko P. Indo, Osamu Inanami, Tomoko Koumura, Shigeaki Suenaga, Hsiu-Chuan Yen, Shizuko Kakinuma, Ken-Ichiro Matsumoto, Ikuo Nakanishi, William St Clair, Daret K. St Clair, Hirofumi Matsui, Richard Cornette, Oleg Gusev, Takashi Okuda, Yasuhito Nakagawa, Toshihiko Ozawa & Hideyuki J. Majima, Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE, Free radical research, 2012, 46(8), 1029-1043

8. Long Binh Vong, Tsutomu Tomita, Toru Yoshitomi, Hirofumi Matsui, Yukio Nagasaki, An Orally Administered Redox Nanoparticle that Accumlates in the Colonic Mucosa and Reduces Colitis in Mice, Gastroenterology, 2012; 143(4); 1027-1036

9. Suemasu S, Yamakawa N, Ishihara T, Asano T, Tahara K, Tanaka K, Matsui H, Okamoto Y, Otsuka M, Takeuchi K, Suzuki H, Mizushima T. Identification of a unique nsaid, fluoro-loxoprofen with gastroprotective activity. Biochem Pharmacol. 2012 84(11):1470-81.

10. Nakamizo H, Suzuki H, Miura S, Mogami S, Kishikawa H, Yoshida H, Matsui H, Hibi T. Transmural pressure loading enhances gastric mucosal cell proliferation.Dig Dis Sci. 2012; 57(10): 2545-54.

11

1. Third Place (Celldance contest), Tsutomu Tomita, Cancer

Dance Movement, The American Society for Cell Biology 52nd Annual Meeting, Dec, 2012

2. Poster Award, Masato Tamura, Hirofumi Matsui, Tsutomu Tomita, Hisato Sadakata, Hideyuki Majima, Hiroko Indo and Ichinosuke Hyodo, ROS Elimination by MnSOD or antioxidants suppressed tumor invasion, 7th International Symposium on Cell/Tissue Injury and Cytoprotection/Organoprotection, Sep, 2012

3.

21 2012 3

1. R. Takagi, J. Fukuda, K. Nagata, Y. Yawata, N. Nomura, and H. Suzuki, Microfluidic microbial culture device for rapid determination of the minimum inhibitory concentration of antibiotics, Analyst, in press

2. N. Mochizuki, T. Kakegawa, T. Osaki, N. Sadr, NN. Kachouie, H. Suzuki, J. Fukuda*, Tissue Engineering Based on Electrochemical Desorption of an RGD-Containing Oligopeptide, Journal of Tissue Engineering and Regenerative Medicine, in press

3. S. Fujita, R. Onuki-Nagasaki, J. Fukuda, J. Enomoto, S. Yamaguchi, M. Miyake, Development of super-dense transfected cell microarrays generated by piezoelectric inkjet printing, Lab on a Chip, 13(1), 77-80 (2013)

4. T. Kakegawa, N. Mochizuki, N. Sadr, H. Suzuki, J. Fukuda*, Cell-adhesive and cell-repulsive zwitterionic oligopeptides for micropatterning and rapid electrochemical detachment of cells, Tissue Engineering, 19(1-2):290-8 (2013)

12

5. J. Fukuda*, S. Takahashi, T. Osaki, N. Mochizuki and H. Suzuki, Processing of nanolitre liquid plugs for microfluidic cell-based assays, Science and Technology of Advanced Materials, 13, 064201 (2012).

6. T. Anada, J. Fukuda, Y. Sai, O. Suzuki, An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids, Biomaterials, 33(33), 8430–41 (2012)

7. S. Kimura, J. Fukuda, A. Tajima, and H. Suzuki, On-chip diagnosis of subclinical mastitis in cows by electrochemical measurement of neutrophil activity in milk, Lab on a Chip, 12 (7), pp.1309-15 (2012)

1) Yoshikawa HY, Cui J, Kratz T, Matsuzaki T, Nakabayashi S, Marx A, Engel U, Lendlein A, Tanaka M. Quantitative evaluation of adhesion of osteosarcoma cells to hydrophobic polymer substrate with tunable elasticity. Journal of Physical Chemistry B 2012; 116: 8024-8030.

2) Yoshikawa HY, Hosokawa Y, Murai, R, Sazaki G, Kitatani T, Adachi H, Inoue T, Matsumura H, Takano K, Murakami S, Nakabayashi S, Mori Y, Masuhara H. Spatially Precise, Soft Microseeding of Single Protein Crystals by Femtosecond Laser Ablation. Crystal Growth & Design 2012; 12: 4334-4339.

3) Koyama H, Nakao H, Yoshikawa HY, Nakabayashi S. Magnetic field induced hopping among Arnold entrainment tongues of coupled iron electrochemical oscillators. 2013; 555: 268-273.

4) Maruyama M, Kawahara H, Sazaki G, Maki S, Takahashi Y, Yoshikawa HY, Sugiyama S, Adachi H, Takano K, Matsumura T, Inoue T, Murakami S, Mori, Y. Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow. Crystal Growth & Design 2012; 12: 2856-2863.

1) Sugiura S., Takagi T., Yamaguchi M., Sumaru K. and Kanamori T., Photodegradable Hydrogel for Optical Control of Cell Adhesion and Detachment. Chem. Mater., submitted

2) Sugiura S., Takagi T., Yamaguchi M., Sumaru K. and Kanamori T., A Photodegradable Hydrogel Sheet for Microscale Optical Control of Cell Adhesion and Detachment, Proc. microTAS 2012, Okinawa, Oct. 2012

Poster Award, Sugiura S., Takagi T., Yamaguchi M., Sumaru K. and Kanamori T., Cell Micropatterning and Manipulation on Photodegradable Hydrogel Sheet, Gelsympo 2012, Tsukuba, Oct. 2012

A03 3

1) Sekine H, Shimizu T, Sakaguchi K, Dobashi I, Wada M,

Yamato M, Kobayashi E, Umezu M, Okano T, In vitro fabrication of functional three-dimensional tissues with perfusable blood vessels. Nat Commun. 2013, 29; 4:

1399-1401. 2) Tamura A, Kobayashi J, Yamato M, Okano T,

Temperature-responsive poly(N-isopropylacrylamide)-grafted microcarriers for large-scale non-invasive harvest of anchorage-dependent cells. Biomaterials 2012; 33: 3803-12.

3) Tamura A, Nishi M, Kobayashi J, Nagase K, Yajima H, Yamato M, Okano T, Simultaneous enhancement of cell proliferation and thermally induced harvest efficiency based on temperature-responsive cationic copolymer-grafted microcarriers. Biomacromolecules 2012; 13: 1765-73.

4) Tang Z, Akiyama Y, Itoga K, Kobayashi J, Yamato M, Okano T, Shear stress-dependent cell detachment from temperature-responsive cell culture surfaces in a microfluidic device. Biomaterials 2012; 33: 7405-11.

5) Idota N, Kikuchi A, Kobayashi J, Sakai K, Okano T, Modulation of graft architectures for enhancing hydrophobic interaction of biomolecules with thermoresponsive polymer-grafted surfaces, Colloid Surf B 2012; 99: 95-101.

6) Tamura A, Kobayashi J, Yamato M, Okano T, Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture, Acta Biomaterialia 2012; 8: 3904-3913.

7) Nishi M, Kobayashi J, Akiyama Y, Yamato M, Yajima H, Okano T, Thermoresponsive affinity interaction between cells and immobilized antibodies on poly(N-isopropylacrylamide)-grafted surfaces. IEEE Proceedings of International Symposium on Micro-NanoMechatronics and Human Science (MHS 2012) 2012: 442-6.

1) Kobayashi J, Okano T, Thermoresponsive thin hydrogel-grafted surfaces for biomedical applications. React Funct Polym (in press)

2) Tang Z, Akiyama Y, Okano T, Temperature-Responsive Polymer Modified Surface for Cell Sheet Engineering, Polymers 2012, 4(3), 1478-1498

1) 6bFGF 2012 7 9-10

2) 41

bFGF 2012 6 25-26

1) 2012.12.22 1 3700 09 1

2) 2013. 1.28 6

1) Anada T, Fukuda J, Sai Y, Suzuki O. An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids. Biomaterials 2012; 33: 8430-41.

2) Shiwaku Y, Anada T, Yamazaki H, Honda Y, Morimoto S, Sasaki K, Suzuki O. Structural, morphological and surface characteristics of two types of octacalcium

13

phosphate-derived fluoride-containing apatitic calcium phosphates. Acta Biomater 2012; 8: 4417-25.

3) Handa T, Anada T, Honda Y, Yamazaki H, Kobayashi K, Kanda N, Kamakura S, Echigo S, Suzuki O. The effect of an octacalcium phosphate co-precipitated gelatin composite on the repair of critical-sized rat calvarial defects. Acta Biomater 2012; 8:1190-200

4) Tanuma Y, Anada T, Honda Y, Kawai T, Kamakura S, Echigo S, Suzuki O. Granule size-dependent bone regenerative capacity of octacalcium phosphate in collagen matrix. Tissue Eng Part A 2012; 18: 546-57

5) Masuda T, Takei N, Nakano T, Anada T, Suzuki O, Arai F. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomed Microdevices 2012; 14: 1085-93.

6) Morimoto S, Anada T, Honda Y, Suzuki O. Comparative study on in vitro biocomatibility of synthetic octacalcium phosphate and calcium phosphate ceramics used clinically. Biomed Mater 2012; 7: 045020

7) Shiraishi N, Tu R, Uzuka R, Anada T, Narushima T, Goto T, Niinomi M, Sasaki K, Suzuki O. Biomechanical evaluation of amorphous calcium phosphate coated TNTZ implants prepared using a radiofrequency magnetron sputtering system. Mater Trans 2012; 53: 1343-48.

8) Miura K, Matsui K, Kawai T, Kato Y, Matsui A, Suzuki O, Kamakura S, Echigo S. Octacalcium phosphate (OCP) collagen composites with titanium mesh facilitate alveolar augmentation in canine mandibular bone defects. Int J Oral Maxillofac Surg 2012; 41: 1161-69.

Suzuki O. Octacalcium phosphate (OCP)-based bone substitute materials. Jpn Dent Sci Rev 2013, in press.

15

1) Ishihara S, Yasuda M, Nishioka T, Mizutani T, Kawabata K, Shirato H, Haga H, Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain. FEBS Letter 2013; (ahead of print).

2) Takemoto K, Mizutani T, Tamura K, Takeda K, Haga H, Kawabata K, The number of cyclic stretch regulates cellular elasticity in C2C12 myoblasts. Open Journal of Cell Biology 2013; (ahead of print).

3) Seito N, Yamashita T, Tsukuda Y, Matsui Y, Urita A, Onodera T, Mizutani T, Haga H, Fujitani N, Shinohara Y, Minami A, Iwasaki N, Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice. Arthritis Rheum. 2012; 64(8): 2579-88.

1) ”2 3”M&M2012

(2012) OS1101.

1) Masuda T, Takei N, Nakano T, Anada T, Suzuki O, Arai F. A microfabricated platform to form three-dimensional toroidal multicellular aggregate. Biomed Microdevices 2012; 14: 1085-93.

2) Owaki H, Masuda T, Kawahara T, Takei N, Miwa-Kodama K, Miyasaka K, Ogura T, Arai F. Organ-explanted bionic simulator (OBiS): concurrent microcardiovascular anastomosis of chick embryo. Proc. of IEEE/RSJ International Conference on Intelligent Robots and Systems 2012; 3812-17.

3) Yamagishi Y, Masuda T, Takei N, Owaki H, Matsusaki M, Akashi M, Arai F. Three-dimensional assembly of multilayered tissues. Proc. of the First CIRP Conference on Biomanufacturing 2013, (Accepted)

4) Owaki H, Masuda T, Kawahara T, Miyasaka K, Ogura T, Arai F. Concurrent connecton of embryonic chick heart using a microfluidic chip for Organ-Explant-Chip. Proc. of the First CIRP Conference on Biomanufacturing 2013, (Accepted)

1) Best Paper Award, 2012 11 (2012 International Symposium on Micro-NanoMechatronics and Human Sciences) “Virus Chromatography; On-chip Diagnosis of Virus Infection from Human Body Fluid”. Niimi M, Masuda T, Kaihatsu K, Kato N, Arai F.

2) IEEE Robotics and Automation Society Japan Chapter Young Award, 2012 10 (The 2012 IEEE/RSJ Int. Conf. on Intelligent Robots and Systems) “Organ-explanted Bionic Simulator (OBiS): Concurrent Microcardiovascular Anastomosis of Chick Embryo”. Owaki H.

1) Sakai S, Inagaki H, Liu Y, Matsuyama T, Kihara T, Miyake J, Kawakami K, Taya M, Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures, Biotechnology and Bioengineering 2012; 109: 2911-2919

2) Silberberg YR, Mieda S, Amemiya Y, Sato T, Kihara T, Nakamura N, Fukazawa K, Ishihara K, Miyake J, Nakamura C, Evaluation of the actin cytoskeleton state using an antibody-functionalized nanoneedle and an AFM, Biosensors and Bioelectronics 2013; 40: 3-9

3) Han SW, Morita K, Simona P, Kihara T, Miyake J, Banu M, Adachi T, Probing Actin Filament and Binding Protein Interaction Using an Atomic Force Microscopy, Journal of Nanoscience and Nanotechnology, (in press)

1) Shinohara S, Shinohara S, Kihara T, Miyake J, Regulation of differentiated phenotypes of vascular smooth muscle cells, Current Basic and Pathological Approaches to the Function of Muscle Cells and Tissues - From Molecules to Humans, Sugi, H. (ed), InTech, pp.331-344 (2012)

1) Best Paper Award 2012 (Winner) of 22nd Anniversary World Congress on Biosensors, Evaluation of the actin cytoskeleton state using an antibody-functionalized nanoneedle and AFM, Silberberg YR, Mieda S, Amemiya Y, Sato T, Kihara T, Nakamura N, Fukazawa K, Ishihara K, Miyake J, 2012.5.17

1) Egusa H, Kobayashi M, Matsumoto T, Sasaki JI, Uraguchi S, Yatani H. Application of cyclic strain for accelerated skeletal

14

myogenic differentiation of mouse bone marrow-derived mesenchymal stromal cells with cell alignment. Tissue Eng A in press

2) Sasaki J, Matsumoto T, Nishiguchi M, Matsusaki M, Egusa H, Nakano T, Akashi M, Imazato S, Yatani H. In vitro reproduction of endochondral occification using 3D cell constructs. Integr Biol 2012; 4: 1207-1214.

3) An SH, Matsumoto T, Miyajima H, Nakahira A, Kim KH, Imazato S. Porous zirconia/hydroxyapatite scaffolds for bone reconstruction. Dent Mate 2012;28: 1221-1231.

4) An SH, Matsumoto T, Sasaki J, Miyajima H, Narayanan R, Imazato S, Kim KH. In vitro bioactivity evaluation of nano- and micro- crystalline anodic TiO2: HA formation, cellular affinity and organ culture. Mater Sci Eng C 2012; 32: 2516-2522.

5) An SH, Matsumoto T, Miyajima H, Sasaki J, Narayanan R, Kim KH. Surface characterization of alkali- and heat-treated Ti with or without prior acid etching. Appl Surf Sci, 2012; 258: 4377-4382.

6) Yoshida Y, Yoshihara K, Hayakawa S, Nagaoka N, Okihara ., Matsumoto T, Minagi S, Osaka A, Van Landuyt K and Van Meerbeek B. HEMA inhibits interfacial nano-layering of the functional monomer MDP. J Dent Res 2012; 91: 1060-1065.

11

2012 9 21

1) Takanori Takebe, Shinji Kobayashi, Hiroomi Kan, Hiromu Suzuki, Mitsuru Mizuno, Yuichiro Yabuki, Takuro Adegawa, Tomohiko Yoshioka, Junzo Tanaka, Jiro Maegawa, Hideki Taniguchi: Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor. Transplant Proc, 44 (4), 1158-1161, 2012

2) Takanori Takebe, Keisuke Sekine, Yuka Suzuki, Masahiro Enomura, Shin Tanaka, Yasuharu Ueno, Yun-Wen Zheng, Hideki Taniguchi: Self-organization of human hepatic organoid by recapitulating organogenesis in vitro. Transplant Proc, 44 (4), 1018-1020, 2012

3) Takanori Takebe, Naoto Koike, Keisuke Sekine, Masahiro Enomura, Yasuharu Ueno, Yun-Wen Zheng, Hideki Taniguchi: Generation of human vascular network in vitro. Transplant Proc, 44 (4), 1130-1133, 2012

4) Keisuke Sekine, Takanori Takebe, Yuka Suzuki, Akihide Kamiya, Hiromitsu Nakauchi, Hideki Taniguchi: Highly efficient generation of definitive endoderm lineage from human induced pluripotent stem cells. Transplant Proc, 44 (4), 1127-1129, 2012

5) Keisuke Sekine, Takanori Takebe, Masahiro Enomura, Chiemi Matsui, Hiroyasu Tanaka, Hideki Taniguchi: Regenerative medicine approach as an alternative treatment to islet transplantation. Transplant Proc, 44 (4), 1104-1106, 2012

6) Hiroyuki Koike*, Koji Kubota*, KeisukeSekine*, Takanori Takebe, Rie Ouchi, Yun-Wen Zheng, Yasuharu Ueno, Naoki Tanigawa, Hideki Taniguchi. (*; Equal contribution) Establishment of automated culture system for murine induced pluripotent stem cells. BMC biotechnology, 12 (1), 81, 2012

1) ”

”, Vol.19 No.1, 2012 , Organ Biology

Young Investigator Award. 11 Jun 12-14, 2012,

1. NATURE, NEWS&VIEWS, Rudimentary liver grown in vitro (by David Cyranoski), Nature doi:10.1038/nature.2012.10848, 20 June 2012

2. Newton iPS 2012.12

3. Newton iPS 2 2012.10

4. New Scientist magazine, Tiny human liver grown inside mouse’s head(by Rob Gilhooly), 21 June 2012

1. 2013. 1.4 2. 10 2012.11.28 3. iPS

2012.7.15 4. iPS

2012.6.29 5. iPS

2012.6.15 6. NYDailyNews.com, Japan team claims they created

functional human liver from stem cells:report, 11 June 2012

7. iPS 2012.6.9

8. iPS 2012.6.9

9. iPS 2012.6.9

10. iPS 2012.6.9

11. AFP iPS2012.6.8

12. iPS (1 ) 2012.6.8

13. iPS 2012.6.8

14. iPS 2012.6.8

15. NHK iPS “ ” 2012.6.8

16. YOMIURI ONLINE iPS …2012.6.8

17. Business Line iPS2012.6.8

, PCT: PCT/JP2012/074840 ( 2012.9.28)

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超高速バイオアセンブラ 第 1回若手研究者シンポジウム 報告

編集後記 平成24年度は計画班の間で，また本年度から参加している公募班との間で活発な連携研究が展開されました．これら新しく立ち上がった萌芽的研究成果を迅速に普及・発展させるため，新井領域代表のリーダーシップのもと，本年度は他の新学術領域との合同公開シンポジウム（ソフトインターフェイス，ナノメディスン，融合マテリアル），ジョイント研究会（少数性生物学），また第１回若手研究者シンポジウムの開催，さらには日本バイオマテリアル学会シンポジウム2012等への参画を通じ，バイオアセンブラ研究の紹介を積極的に行いました．今回のサーキュラーはその一端を纏めております．詳細はホームページ http://bio-asm.jp/ をご覧下さい．次年度の中間評価に向けて班員の皆様の益々の研究推進を期待しております．（鈴木記）

第4回バイオアセンブラシンポジウム 主催：新学術領域「バイオアセンブラ」総括班 日時：平成25年3月10日（日） 13:00－16:40 場所：有馬温泉 向陽閣 会議室

Information

超高速バイオアセンブラ Newsletter No. 4 2013.3

16

＜プログラム＞ 領域代表挨拶 13:00-13:15 領域代表 大阪大学 新井健生 講演 13:15-13:35 講演1「細胞シート用ヘラ機構“Cell Scooper”」 A01班 大阪大学・助教 多田隈 建二郎 13:35-13:55 講演2「微細加工技術を用いた細胞培養チップデバイス」 A02班 横浜国立大学・准教授 福田淳二 13:55-14:15 講演3「ヒト幹細胞から臓器を創る！」 A03班 横浜市立大学・助手 武部貴則 14:15-14:35 休憩 特別講演 14:35-15:35 特別講演１「培養屋からみた細胞挙動解析と細胞製造技術」 大阪大学・教授 紀ノ岡 正博 15:35-16:35 特別講演２「分子・細胞・組織を操る集学的アプローチ」 金沢工業大学・教授 松田 武久 閉会挨拶 16:35-16:40 名古屋大学・教授 福田敏男

皆様へのお願い 1. 研究成果を論文として発表された場合には，その1ページ目（英文）のコピー（PDFでも可）と 和文要旨（題目，著者名，所属，要旨）をお送りください．次号で紹介させていただきます． 2. 新聞ニュースや海外出張報告などもどしどしお送りください． 送り先は以下の通りです． 〒980-8575 仙台市青葉区星陵町4-1 東北大学大学院歯学研究科 顎口腔機能創建学分野 鈴木治 suzuki-o@m.tohoku.ac.jp

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