2012-iGEM Break Symmetry Southeast university. 凌昕彤 周醒驭 陈牧原 高 珅 Team members...
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Transcript of 2012-iGEM Break Symmetry Southeast university. 凌昕彤 周醒驭 陈牧原 高 珅 Team members...
2012-iGEMBreak Symmetry
Southeast university
凌昕彤
周醒驭
陈牧原
高 珅
Team members
周 乐 程 鹏
桂 凯
张 俊 樊雪龙
徐小丹
贾正阳
Technical Background
Design Scheme
Results && Biobricks &&Potential Work
ContentsContents
Biosafety &&Human Practice
Experiment
Technical BackgroundTechnical Background
Create a star!
Main GoalMain Goal : Engineering bacteria colony to automatically break their own symmetry
: Engineering bacteria colony to automatically break their own symmetry
Whole System
AHL transduction
Light sensor
Toggle switch
Toggle switch
Antisense FtsZ repress division
Antisense FtsZ repress division
FtsZ mRNA
FtsZ asRNA
FtsZ geneFtsZ protein
silencing
transcription
translate
Tby gene transcription
Lux I gene Lux I protein
SAM
AHL
luxR gene express luxR protein
combine
Luxpr promoterpromote
pcI promoter
Lac promoter
lacI promoter
lacI gene
cI gene cI protein
lacI proteinpromote
promoteinhibit
inhibit
IPTG
ultraviolet rays
promote
Light sensor promoter
cI gene cI proteinpromote
Light receiverexpress
Red light
OmpC OmpC
express
express
express
express
Light Sensor
Red Light11
Blue Light22
Red Light
Light Sensor
Cph8Cph8
RegulatoryBLp
ycgZ ycgF ycgE
regulated by the ycgF/E system
Blue Light
Conformation
Binding&&Repress Expression
Blue Light
Light Sensor
Toggle Switch
Toggle Switch
lacI+pL LuxR LuxIGenerating More AHL
Activating
AHL-Based Quorum Sensing
Antisense RNA
Antisense RNA
Verification for Antisense RNA
IPTG=0
After 10 hours in 37°C environment
IPTG=100 IPTG=200
Design Scheme
Related Kits:
j5526=r11+b34+mRFP+tt i13507=b34+mRFP+tt i13521=tetR+b34+mRFP+tt j04450=lac+b34+mRFP+tt
r0011=lacipl r0051=pci r0040=ptetr r0063=luxpl(ahl--) r0062=luxpr(ahl++) r1051=r0051=pcI
i732820=b34+LacI+tt j23039=tetR+b34+luxI+tt i13973=pcI+b34+yfp+tt k098991=pcI+b34+gfp+tt p0451=b34+cI+tt k092400=b34+luxi+tt i13202=lacipl+b34+luxI+tt
t9002=tetr+b34+luxr+tt+luxpr+b32+gfp+tt f2620=ptetr+b34+luxr+tt+luxpr
k145270=pro+b34+luxr+tt j37033=b34+luxr k091204=pro+b34+luxr+tt i0462=b34+luxr+tt k116640=ptetR+b32+luxr+tt j37015=ptetR+b34+luxr+tt+luxpr+b34+luxI+b34+gfp+tt
K088006=b33+lacI+lac+b34+cI+b32+gfp+tt+pcI+b34+rfp+tt
I763026=ptetR+b34+lacI+tt I763035=ptetr+b34+lacI+b34+gfp+tt p0151=b31+cI+tt p0440=b34+tetr+tt
k145205=ptetr+b32+gfp+tt K082034=lac+b34+gfp+tt
The Simulation Experiment
The Micro-Model
-Based on Cellular Automaton
The Macro-Model
-Based on Improved Cellular
Automaton
Modeling
Approximate Bayesian Computation ( ABC ) for parameter inference in gene expression model
Modeling
1. Approximate Bayesian computation (ABC)
ABC rejection sampler
ABC MCMC
2. Gene expression model
Modeling
Results
Results
Verification for Antisense RNA
IPTG=0
After 10 hours in 37°C environment
IPTG=100 IPTG=200
others
Potential applications
Biosensor
Cell Printing
Signal Transnduction
Potential applications
Acknowledgements
We would like to thank :Academy of Biomedical Engineering for all these support;
数学模型数学模型
文档文档
反义反义
光敏启动光敏启动
开关开关
AHLAHLOthers
③
②
①
该项目大部分由我们自主完成(除了非天然序列发夹结构外包合成),技术上不依赖外援。
项目总结 由于缺乏经验,该项目实现效果不如我们预期,但已部分实现。
下一步我们将完成元件的标准化和功能验证,以及各种材料的整理和项目展示工作。