KorbanBBtskpengadilan

Penerapan ilmu genetika : untuk mengidentifikasi individu dengan menganalisa profil DNA.Melacak,Paternalitas,Maternalitas,Mutilasi,Perkosaan (seksual),Database,DVI.

DNA IN THE CELL*

Dimanakah letak DNA

RNA & DNA

Codon : Ikatan yang terdiri dari 3 buah basa yang mengkode asam amino tertentu

Daerah yang mengkode AATidak mengkode AA

Forensic DNA HereditasHanya diwariskan dari orang tua ke anakAnak laki laki atau perempuan mempunyai komposisi DNA gabungan dari kedua orang btuanyaIdentifikasi melalui DNA sangat besar nilai pembedanyaDNA tidak tahan lama, mudah busuk dan kontaminasi.Hanya ada dua kopy, satu dari ayah satu yang lainnya dari ibu.*1. NuDNA

childMother(known parent)Allegedfather?

Rules of Inheritance1) Child has two alleles for eachautosomal marker (one frommother and one frombiological father)2) Child will have mothersmitochondrial DNA haplotype(barring mutation)

3) Child, if a son, will havefathers Y-chromosomehaplotype (barring mutation)RandommanAllegedmother

MissingAllegedfather?Parentage (Paternity) TestingReverse Parentage Testing(Missing Persons Investigation)(A)(B)child

Figure 23.1, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition 2005 Elsevier Academic Press

22Linkage analysisNu. DNA

1213

Family problem

SpouseSonDaughterFather

Missing IndividualMother

BrotherNieceNephewUncle

SisterAunt

CousinFigure 24.2, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition 2005 Elsevier Academic Press5/29/2010DNA LAB of DOKPOL By Put30Reference sample

Grandmother

Terget DNA

2. MtDNAHanya diwariskan dari ibu ke anaknyaJika diturunkan kepada anak laki-laki maka stop tidak diturunkan kepada cucunya.Pada anak perempuan mtDNA diteruskanMaka mitochondrial DNA bukan untuk membedakan*DNA LAB of DOKPOL By Put*

DNA LAB of DOKPOL By Put

1211103

9681817151613ABB

C4

C

C5

DBBBBBB7

E12

FG14

GGFigure 10.2, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition 2005 Elsevier Academic PressMt DNAMt DNA Haplotype Groups

Mitocondrial DNAHanya diwariskan oleh IBU kepada anaknya

?uncle3rd cousinFigure 9.3, J.M. Butler (2005) Forensic DNA Typing, 2nd Edition 2005 Elsevier Academic PressY-chromosome

Killed July 22, 2003Captured December 13, 2003

Matching Y-STRHaplotype Used toConfirm Identity

(along with allele sharingfrom autosomal STRs)

Uday and Qusay HusseinIs this man reallySadaam Hussein?

Butler, J.M. (2005) Forensic DNA Typing, 2nd Edition, Box 23.1, p. 534

SARINA RAHMAH

YDAUDKHAULAHRAHMA BT RUSDI

YMUAWWIDZNote : Daud & Muawwidz have the identically Y chromosome

NoordinM TopDulmatin

Matching processCilacap

Johor

Lab CodeD8S1179D21S11D7S820CSF1POD3S1358TH01D13S317D16S539Suspect1114282881111121617798810 13DAUD141428308106111717910891213KHAULAH111428308121111171799.3891012ARINA RAHMAH12143030101261116179.310912912

Lab CodeD2S133819S433vWATPOXD18S51AMELD5S818FGASuspect1919141417189111516X Y101221 27DAUD192413141818891618XY10122021KHAULAH192213141418891516XX12132027ARINA RAHMAH222413141418881518XX10132022

Lab CodeD8S1179D21S11D7S820CSF1POD3S1358TH01D13S317D16S539RAHMA bt RUSDI1414323311131011151599891213MUAWWIDZ141428338111011151679881213Suspect1114282881111 12161779881013

Lab CodeD2S1338D19S433vWATPOXD18S51AMELD5S818FGARAHMA bt RUSDI202291518188121419XX10102123MUAWWIDZ19209141818891415XY10102123Suspect1919141417189111516XY10122127

SALI USMANMASNIATI

Matching processPamulang.

Lab CodeD2S1338D19S433vWATPOXD18S51AMELD5S818FGASon172013131617881216XY10132223Suspect1723131316188101216XY12132224Mam17191313.2161910111216XX13132324

Lab CodeD8S1179D21S11D7S820CSF1POD3S1358TH01D13S31716S539Son131429298812121616778111012Suspect10132929881112151679811910Mam101529308111012151578811911

5. Sampel DNA

HOW TO COLLECT DNA FOR THE USED OF EXAMINATIONS*DNA LAB of DOKPOL By Put*BloodSemenSalivaUrineHairTeethBoneTissueTraceCigarette buttSaliva

Sources of Biological Evidence

TYPE SAMPELEvidence sample: sampel TKPReference sample: Sampel PembandingDirect reference sample langsungFamily keluarga

**

Kondisi mayatSampel yang diinginkanTidak busuk, body utuhDarah (on FTA card or swab) and buccal (mouth) swabsTidak busuk, hancurJika mungkin, darahdanJaringan otot, dalam, merah (~1.0g)Busuk, body utuh Atau hancurtulang panjang. (cut 4-6cm, using window cut without separating the shaft)And/orGigi sehat (molars preferable)And/orTulang lainnya. (~10g, if possible; dense cortical bone preferable)Tubuh terbakarAll samples aboveOrSwab dari bagian dalam kantong urine

*DNA LAB of DOKPOL By Put*

DNA qualityCommonly availableMight be availableGOOD sources of DNA- SIKAT GIGI- PENCUKUR JENGGOT- samples from a bone marrow donor program, blood cards from PKU newborn screening, - national biobanks, criminal databases*, paternity testing labs*, reference samples from military personnel*- other clinical blood or serum samples- sperm bank samples- dried umbilical cord - paraffin embedded pathology specimenMEDIUM sources of DNA- SISIR- lipsticks, deodorant sticks- BANTAL- TOPI, GELAS- PEDALAMAN KOTOR- cervical smears- fingernail clippings- PUNTUNG ROKOK- PIPA- mouth piece, mouth guard- motorcycle and other sport helmets - caps and hats- inner clothing items (bra, t-shirt, socks)- ear plugs, ear phones- eye glasses- pen with teeth marks- mailed envelopes or postcardsPOOR sources of DNA- jewelry- wrist watches- outer clothing- towels- shoes- hair bands or ear muffs- baby hair- dentures- hair rollers- trimmers, scissors, nail files

*DNA LAB of DOKPOL By Put*

DNA qualityCommonly availableMight be availableGOOD sources of DNA- SIKAT GIGIPENCUKUR JENGGOTSWAB MULUT- samples from a bone marrow donor program, blood cards from PKU newborn screening, - national biobanks, criminal databases*, paternity testing labs*, reference samples from military personnel*- other clinical blood or serum samples- sperm bank samples- dried umbilical cord - paraffin embedded pathology specimen

*DNA LAB of DOKPOL By Put*

DNA qualityCommonly availableMight be available

MEDIUM sources of DNA- SISIR- lipsticks, deodorant sticks- BANTAL- TOPI, GELAS- PEDALAMAN KOTOR- cervical smears- fingernail clippings- PUNTUNG ROKOK- PIPA- mouth piece, mouth guard- motorcycle and other sport helmets - caps and hats- inner clothing items (bra, t-shirt, socks)- ear plugs, ear phones- eye glasses- pen with teeth marks- mailed envelopes or postcards

*DNA LAB of DOKPOL By Put*

DNA qualityCommonly availableMight be availablePOOR sources of DNA- jewelry- wrist watches- outer clothing- towels- shoes- hair bands or ear muffs- baby hair- dentures- hair rollers- trimmers, scissors, nail files

*DNA LAB of DOKPOL By Put*Reference sample

DNA LAB of DOKPOL By Put

Metoda pengambilan sampelSampel langsung:Seluruh sampelmenggunting.Mengambil sampel secukupnyaSwab :Type lift

Storage Suhu ruangan : harus kering4C : kurang dari 2 minggu-20C : kurang dari 9 minggu-80C : jangka waktu lama.

Prinsip penyimpanan: memnghentikan proses degradasi*

Transportasi.Chain of custody !!!!!!!!!= Sejarah perjalanan DNA

Keuntungan DNAJumlah sampel kecil Mungkin: sampel busuk, DNA terawetkan pada beberapa jaringan, Mudah menemukan sampel pembanding, Hi power discrimination. *

Kerugian DNAtidak tahan panas, formalin, alcohol, kontaminasi dengan DNA lain pemeriksaan DNA itu mahal.

*

Samples conditionSegarPembusukan :Internal ~ enzimExternal ~ micro organismeKebakaranRadiasiOrganisme Dan kondisi lingkungan lainnya.

6. Forensic DNA Method

Prioritas sampelSampel langsungSempel pembandingProperty

Property

*DNA LAB of DOKPOL By Put*

DNA qualityCommonly availableMight be availableGOOD sources of DNA- SIKAT GIGI- PENCUKUR JENGGOT- samples from a bone marrow donor program, blood cards from PKU newborn screening, - national biobanks, criminal databases*, paternity testing labs*, reference samples from military personnel*- other clinical blood or serum samples- sperm bank samples- dried umbilical cord - paraffin embedded pathology specimenMEDIUM sources of DNA- SISIR- lipsticks, deodorant sticks- BANTAL- TOPI, GELAS- PEDALAMAN KOTOR- cervical smears- fingernail clippings- PUNTUNG ROKOK- PIPA- mouth piece, mouth guard- motorcycle and other sport helmets - caps and hats- inner clothing items (bra, t-shirt, socks)- ear plugs, ear phones- eye glasses- pen with teeth marks- mailed envelopes or postcardsPOOR sources of DNA- jewelry- wrist watches- outer clothing- towels- shoes- hair bands or ear muffs- baby hair- dentures- hair rollers- trimmers, scissors, nail files

propertyp

propertym

propertym

1234HelmTeleponLip stikK.M. Bacaetsd

4123ggp

Pandangan umumClose Up

?MaskerHand glove

MasukkanDalamTabung steril

Tabung steril yang aku sukaiNamanya Vacuum Tube

Tes golongan darah ABOTes DNA

Swab kit.

Cotton butt

Di dalam tas yang mana terdapat DNACoba cari

Gelas yang mana ???

Ferry young man

Jantung atau pilih pembuluh darah yang paling dekat dengan jantung (dokter).

Lidi kapas kering

Bagian tubuh mana ?

LABEL BB?

Ini Baru BB Tulang Lunakkontaminasi

Benar dan syah

OvercoatHand gloveMasker.

Siapapun dapat menjadi sampel DNASee anything Sir ?

Siapapun mau padaku

*****All DNA SamplingBy Put**********All DNA SamplingBy Put*****All DNA SamplingBy Put*************All DNA SamplingBy Put***All DNA SamplingBy Put*All DNA SamplingBy Put*All DNA SamplingBy Put*All DNA SamplingBy Put*All DNA SamplingBy Put*All DNA SamplingBy Put**All DNA SamplingBy Put**All DNA SamplingBy Put*All DNA SamplingBy Put*****All DNA SamplingBy Put*********************************************