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13 dioxin detection :

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(dioxin) : 210 75 polychlorinated dibenzo- -dioxins PCDDs 135 polychlorinated dibenzofurans PCDFs 210 17 2,3,7,8- (2,3,7,8-TCDD) 209 (PCB) 12 , (dioxin-like congener PCB)

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2,3,7,8- (2,3,7,8-TCDD)

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toxic equivalent quantities TEQ 2,3,7,8-TCDD toxic equivalent quantities toxic equivalent factor TEF PCDDs 2,3,7,8-TCDD PCDDs PCDFs TEF TEQ TEQ

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Toxic Equivalent Factors

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/ HRGC/HRMS 2~3

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Chemically Activated Luciferase Expression CALUX CALUX H4IIE rat-hepatoma H4IIE luciferase gene XRE CALUX 3~4

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Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence. One famous example is the firefly luciferase. In luminescent reactions, light is produced by the oxidation of a luciferin luciferin + O2 oxyluciferin + light luciferin + ATP luciferyl adenylate + PPi luciferyl adenylate + O2 oxyluciferin + AMP + light

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Enzyme-linked immunosorbentassay ELISA Electrochemical biosensor Surface plasmon resonance immunosensor Piezoelectric quartz crystal microbalance biosensor 2,3,7,8-TCDD AhR- ARNT

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dioxin binding peptide peptide library Combinatorial Chemistry

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Combinatorial Chemistry Merrifield: the solid-phase synthesis of peptides. Furka, 1988 : divide- couple-recombine, mixing- divide-couple Houghten, 1991 : mesh packets (Nature), positional scanning