1 Yeast expression of cellulase complex from rumen fungi for direct fermentation of cellulose to...
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Transcript of 1 Yeast expression of cellulase complex from rumen fungi for direct fermentation of cellulose to...
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Yeast expression of Yeast expression of cellulase complexcellulase complex from rumen fungi for from rumen fungi for direct fermentationdirect fermentation of of
cellulose to cellulose to ethanolethanol
Speaker: Speaker: Jeng-Chen Liu(Jeng-Chen Liu( 劉政劉政成成 ))
Student ID: P9921003Student ID: P9921003
Graduate Institute of Bioresources, National Pingtung University of Science and Technology
Proposal for Ph. D. ProgramProposal for Ph. D. Program
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OutlineOutline
BackgroundBackground: : fuel requirement & bioethanolfuel requirement & bioethanol GoalGoal: : direct microbial conversiondirect microbial conversion Materials and methodsMaterials and methods: : gene sources & expressiongene sources & expression Expected resultsExpected results: : cellulose cellulose ethanol ethanol
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Petrolic resources
The problems of petroleum utilizationThe problems of petroleum utilization
Exhaustion
Global warming
CO2+H2Ofossil fuelsfossil fuels
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The cycle of biomass energyThe cycle of biomass energy
CO2+H2O
Photosynthesis
SugarStarchLignocellulose Lipid
Biofuelseg. bioethanol
RenewableRenewable Net emission(CONet emission(CO22)=0)=0
Ethanol-gasoline
eg. E3
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Bioethanol: first generationBioethanol: first generation
Food: sugar starch bean
Food price
Unauthorized cultivation
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Bioethanol: second generationBioethanol: second generation
Food: sugar starch bean
Food price
Unauthorized cultivation
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Bioethanol: second generationBioethanol: second generation
Food price
Unauthorized cultivation
Lignocellulose
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The contents of lignocelluloseThe contents of lignocellulose
(Murphy and McCarthy, 2005)(Prasad et al., 2007)
2 M ton * 40%=800 K ton
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Lignocellulose Lignocellulose ethanol ethanol
Pretreatment:Pretreatment: removed lignin & hemicelluloseremoved lignin & hemicellulose
Hydrolysis:Hydrolysis: cellulose cellulose glucose glucose
Fermentation:Fermentation: glucose glucose ethanol ethanol
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PretreatmentPretreatment
(Lu and Mosier, 2008)
Physical methodsChemical methodsBiological methods
Lignin
Hemicellulose
Cellulose
Ethanol
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Cellulose Cellulose glucose glucose
Acidic hydrolysis Enzymatic hydrolysis
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Table 1. Comparison of hydrolysis methods for cellulose hydrolysis
(Bungay, 2004)
Conversion of cellulose to glucose
Cellulose Glucose
Acid hydrolysisEnzymatic hydrolysis?
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Lignocellulose Lignocellulose ethanol(SHF;SSF;DMC) ethanol(SHF;SSF;DMC)
Pretreatment:Pretreatment: removed lignin & hemicelluloseremoved lignin & hemicellulose
Hydrolysis:Hydrolysis: cellulose cellulose glucose glucose
Fermentation:Fermentation: glucose glucose ethanol ethanol
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Lignocellulose Lignocellulose ethanol(SHF) ethanol(SHF)
PretreatmentPretreatment
Hydrolysis: cellulase supplementationHydrolysis: cellulase supplementation
Fermentation: microorganismFermentation: microorganism
Cellulose ethanol
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Lignocellulose Lignocellulose ethanol(SSF) ethanol(SSF)
PretreatmentPretreatment
Hydrolysis: cellulase supplementationHydrolysis: cellulase supplementation
Fermentation: microorganismFermentation: microorganism
Cellulose ethanol
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Lignocellulose Lignocellulose ethanol(DMC) ethanol(DMC)
PretreatmentPretreatment
Hydrolysis: cellulaseHydrolysis: cellulase
Fermentation: microorganismFermentation: microorganismGlucose
Cellulose
ethanol
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The analysis of enzyme hydrosylate The analysis of enzyme hydrosylate and fermentation productsand fermentation products
(劉, 2010)
(Chen et al., 2003)
Cellulase complex
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Glucose Glucose ethanol ethanol
Saccharomyces cerevisiaeSaccharomyces cerevisiae
ethanol producerethanol producerethanol tolerant ethanol tolerant GRASGRAS
Zymomonas mobilisZymomonas mobilis
Escherichia coliEscherichia coli
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Lignocellulose Lignocellulose ethanol(DMC) ethanol(DMC) PretreatmentPretreatment
HydrolysisHydrolysis
Fermentation:Fermentation: S. cerevisiaeS. cerevisiae
DMCDMC
Glucose
Cellulose
ethanol
GoalGoal
2020
Lignocellulose Lignocellulose ethanol(DMC) ethanol(DMC) PretreatmentPretreatment
Hydrolysis: cellulaseHydrolysis: cellulase
Fermentation:Fermentation: S. cerevisiaeS. cerevisiae
DMCDMC
Glucose
Cellulose
ethanol
GoalGoal
Cellulase complex gene from rumen fungi
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ProtocolsProtocols Rumen fungi
cellulase complex genescellulase complex genes
E. coli expression system
S. cerevisiae expression system
Biochemical characterization
Promoter Cell surface display system
Direct microbial conversion
Bioethanol
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Lignocellulose Lignocellulose ethanol(DMC) ethanol(DMC) PretreatmentPretreatment
Hydrolysis: cellulaseHydrolysis: cellulase
Fermentation:Fermentation: S. cerevisiaeS. cerevisiae
DMCDMC
Glucose
Cellulose
ethanol
Cellulase complex gene from rumen fungi
Expected resultsExpected results
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Thanks for Thanks for your attentionyour attention