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bit H'max J

-A1 2008/7/17 0-1 16 3.6 2.26 50.2 5.7 � �

-A1 2008/7/17 1-2 � � 1.21 41.8 5.4 � �

-A2 2008/7/17 0-1 17 4.2 1.51 50.3 6.1 � �

-A2 2008/7/17 1-2 � � 0.92 58.9 6.0 � �

-R1 2008/7/17 0-1 18 4.2 0.01 12.4 1.5 � �

-R1 2008/7/17 1-2 � � 0.00 12.3 1.5 � �

-R2 2008/7/17 0-1 18 4.4 0.02 19.0 2.1 � �

-R2 2008/7/17 1-2 � � 0.01 19.4 2.16 � �

-1 2008/8/28 0-1 9 6.3 0.00 2.0 0.40 3538 64 10.7 4.87 6.0 0.81

-2 2008/8/28 0-1 13 6.4 0.02 1.9 0.39 6138 77 13.1 4.94 6.3 0.79

-1 2008/8/28 0-1 18 5.6 0.07 11.2 1.52 5475 57 19.3 4.22 5.8 0.72

-2 2008/8/28 0-1 19 6.0 0.07 9.8 1.29 4988 54 15.2 4.29 5.8 0.75

-1 2008/8/28 0-1 15 4.4 1.93 41.7 4.45 200 6 10.1 1.80 2.6 0.70

-2 2008/8/28 0-1 16 4.5 0.94 34.7 3.54 188 7 0.9 2.55 2.8 0.91

-1 2008/8/28 0-1 19 2.5 2.42 54.4 6.07 13 1 0.0 0.00 0.0

-2 2008/8/28 0-1 18 2.4 0.79 39.2 4.87 1000 11 2.1 2.27 3.5 0.65

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3) 4) 0 1 cm 5 201H AVS (mg S/g dry) 6)

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AVS� 0.01 0.70 mg/g

dry

AVS 0.5 mg/g dry 2010 2 15

13.8 , 22m 0.71 mg/g dry

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DNA

DNA

DNA

DNA DNA

21

FastDNA Spin Kit for Soil DNA

AVS = 0.0 mg/g S-19 AVS = 0.31 mg/g

A2 AVS = 1.54 mg/g


T.-Hoshino et al., Microbes

Environ. 19: 13-19, 2004 DNA real-time PCR

dsrA

DNA dsrA

DNA FastDNA Spin Kit for Soil

DNA

DNA

1 DNA DNA

DNA

S-19 DNA t

5%

dsrA

DNA real-time PCR

dsrA 2 DNA

dsrA dsrA

t 5%

DNA FastDNA Spin Kit for

Soil DNA

1� DNA FastDNA Spin Kit for Soil 0.5 g

3

2� DNA FastDNA Spin Kit for Soil 0.5 g

3

22

FastDNA Spin Kit for Soil DNA DNA

16S rDNA PCR DNA

2009 11 2010 8 55 DNA 3

165 16S rDNA

PCR DNA

DNA PCR

1 FastDNA Spin Kit for Soil 3 DNA

DNA TE buffer 10 100 PCR

16S rDNA

PCR DNA 1.5 kbp PCR 1

1� PCR

TE 10 TE 100

H1 H32 H33 H36 H43 H44 2010K1 2010K2 2010K3 2010K4 2010K5 2010K6 2010K7 2010K8

F7 F11 F17 F21 F23 Fcont

2010 2 15

2010 8 17

2010 2 15

1 2

1 2

1 2

1 2

1 2

1 2

1 2

1 2

1 2

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2010 1 13

2010 8 31

2009E Nov1 Nov2 2010E Jan1 Jan2 2010E Mar1 Mar2 2010E Jun1 Jun2 2010E Jul1 Jul2 2010E Aug1 Aug2

2009 11 27

2010 1 27

2010 3 30

2010 6 2

2010 7 26

2010 8 20

2010E Aug3 Aug4 Aug5

2010 8 19

PCR

DNA FastDNA Spin

Kit for Soil DNA

3 DNA qPCR

dsrA DNA

dsrA DNA


2009 2010 DNA PCR

DNA

DNA

-85ºC

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