LC Chen at BIME NTU

實驗數據分析與圖表製作實驗數據分析與圖表製作Data Anal and FigTable Data Anal and FigTable PrepsPreps

Lin-Chi Chen ( 陳林祈 )

Dept of Bio-Industrial Mechatronics EngNational Taiwan University

Email chenlinchintuedutw Tel 02-3366-5343

20082008 生物產業機電工程特論 生物產業機電工程特論 (840 ndash 930 am on Oct 23 2008)(840 ndash 930 am on Oct 23 2008)

LC Chen at BIME NTU

LC Chen at BIME NTU

Structure of a Scientific Paper

Most journals will require the same basic sections

1048708Abstract （摘要）1048708Introduction （導論） Background + Objective(s)

1048708Materials and Methods （研究方法）1048708Results （結果）1048708Discussion （討論）1048708Conclusion(s) （結論）1048708References (Literature Cited) （參考文獻）1048708Tables Figures Figure Legends （圖表及說明）

LC Chen at BIME NTU

Planning for a Scientific Manuscript

Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript

bull Number of illustrationsfigures

bull Number of tables

bull Format of final submission

bull Check journalrsquos Web site or in journal itself for these and other specifications

The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)

bull Presentation of the evidence

bull Validity of the evidence

bull Implications of the evidence

bull Assessment of answerrsquos validity

bull Conclusion

Require Figures and Tables in Support of the ldquoFindingsrdquo

LC Chen at BIME NTU

資料來源蘋果日報

How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

LC Chen at BIME NTU

Structure of a Scientific Paper

Most journals will require the same basic sections

1048708Abstract （摘要）1048708Introduction （導論） Background + Objective(s)

1048708Materials and Methods （研究方法）1048708Results （結果）1048708Discussion （討論）1048708Conclusion(s) （結論）1048708References (Literature Cited) （參考文獻）1048708Tables Figures Figure Legends （圖表及說明）

LC Chen at BIME NTU

Planning for a Scientific Manuscript

Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript

bull Number of illustrationsfigures

bull Number of tables

bull Format of final submission

bull Check journalrsquos Web site or in journal itself for these and other specifications

The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)

bull Presentation of the evidence

bull Validity of the evidence

bull Implications of the evidence

bull Assessment of answerrsquos validity

bull Conclusion

Require Figures and Tables in Support of the ldquoFindingsrdquo

LC Chen at BIME NTU

資料來源蘋果日報

How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Structure of a Scientific Paper

Most journals will require the same basic sections

1048708Abstract （摘要）1048708Introduction （導論） Background + Objective(s)

1048708Materials and Methods （研究方法）1048708Results （結果）1048708Discussion （討論）1048708Conclusion(s) （結論）1048708References (Literature Cited) （參考文獻）1048708Tables Figures Figure Legends （圖表及說明）

LC Chen at BIME NTU

Planning for a Scientific Manuscript

Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript

bull Number of illustrationsfigures

bull Number of tables

bull Format of final submission

bull Check journalrsquos Web site or in journal itself for these and other specifications

The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)

bull Presentation of the evidence

bull Validity of the evidence

bull Implications of the evidence

bull Assessment of answerrsquos validity

bull Conclusion

Require Figures and Tables in Support of the ldquoFindingsrdquo

LC Chen at BIME NTU

資料來源蘋果日報

How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Planning for a Scientific Manuscript

Determine journalrsquos requirements (Instructions to Authors)bull Length of manuscript

bull Number of illustrationsfigures

bull Number of tables

bull Format of final submission

bull Check journalrsquos Web site or in journal itself for these and other specifications

The manuscript should be sequential and logical includingbull Statement of problem (hypothesis)

bull Presentation of the evidence

bull Validity of the evidence

bull Implications of the evidence

bull Assessment of answerrsquos validity

bull Conclusion

Require Figures and Tables in Support of the ldquoFindingsrdquo

LC Chen at BIME NTU

資料來源蘋果日報

How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

資料來源蘋果日報

How do nowadays newspapers present the figures and tables in support of the ldquostoriesrdquo determined by the editor

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

httpabacusbatesedu~gandersobiologyresourceswritingHTWtablefigshtml

The way to present the figures and tables in support of the ldquofindingsrdquo by scientific researchers is somewhat different

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Three Forms of Data Presentation

Text contrary to what you may have heard not all analyses or results warrant a Table or Figure Some simple results are best stated in a single sentence with data summarized parenthetically

Seed production was higher for plants in the full-sun treatment (523 +-68 seeds) than for those receiving filtered light (147+- 32 seeds t=118 df=55 plt0001)

Tables Tables present or summarize lists of numbers or text in columns each column having a title or label Do not use a table when you wish to show a trend or a pattern of relationship between sets of values - these are better presented in a Figure

Figures Figures are visual presentations of results including graphs diagrams photos drawings schematics maps etc Graphs are the most common type of figure Graphs show trends or patterns of relationship

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

How to Refer Tables and Figures

How to refer to Tables and Figures from the text Every Figure and Table included in the paper MUST be referred to from the text Use sentences that draw the readers attention to the relationship or trend you wish to highlight referring to the appropriate Figure or Table only parenthetically

Ex Germination rates were significantly higher after 24 h in running water than in controls (Fig 4)

Ex DNA sequence homologies for the purple gene from the four congeners (Table 1) show high similarity differing by at most 4 base pairs

Avoid sentences that give no information other than directing the reader to the Figure or Table

Ex Table 1 shows the summary results for male and female heights at Bates College

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Numbering Placement amp Foolproof

How to number Tables and Figures Figures and Tables are numbered independently in the sequence in which you refer to them in the text starting with Figure 1 and Table 1

Placement of Figures and Tables within the Paper In consideration of your readers place each Table or Figure as near as possible to the place where you first refer to it (eg the next page) For manuscripts (eg lab papers) Tables and Figures are usually put on separate pages from text material

The Acid Test for Tables and Figures Any Table or Figure you present must be sufficiently clear well-labeled and described by its legend to be understood by your intended audience without reading the results section ie it must be able to stand alone and be interpretable Overly complicated Figures or Tables may be difficult to understand in or out of context so strive for simplicity whenever possible If you are unsure whether your tables or figures meet these criteria give them to a fellow biology major (not in your course) and ask them to interpret your results

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Descriptive Legends and Captions

bull Descriptive Legends or Captions To pass the acid test above a clear and complete legend (sometimes called a caption) is essential Like the title of the paper itself each legend should convey as much information as possible about what the Table or Figure Do not simply restate the axis labels with a versus written in between

bull Example Figure 1 Height frequency () of White Pines (Pinus strobus) at Thorncrag Bird Sanctuary Lewiston Maine before and after the Ice Storm of 98 Before n=137 after n=133 Approximately 16 of canopy pines were topped by heavy ice loads averaging 36 m stem length decrease Four trees fell during the storm and were excluded from the post-storm survey

Where do you place the legend

bull Table legends go above the body of the Table and are left justified Tables are read from the top down

bull Figure legends go below the graph graphs and other types of Figures are usually read from the bottom up

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Table Prep Rule of Thumb

bull Determine journalrsquos limitations on number style or structure of tables

bull Tables should be useful (not too simple) and easy to understand (not too complex)

bull Use tables when exact values are important

bull Use tables when they are more efficient to summarize results than in long complex sentences

bull Tables with footnotes and abbreviations should be easily understandable

bull General rule 1 table per 1000 words of text

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Anatomy of Table

The typical layout of a table in three sections demarcated by lines

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Tables are most easily constructed

using your word processors table

function or a spread sheet such as

Excel Gridlines or boxes commonly

invoked by word processors are

optional for our purposes but

unlikely to be permitted in a journal

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Example Literature Comparison

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

units are specified in column headings wherever appropriate

Example Summary of Readings

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Types of illustrations

bull Chartsgraphs (bar graph frequency histogram XY scatter plot XY line graph)

bull Photographs (including X-rays SEM DNAprotein image)

bull Line drawings (schematic illustration instrumentation)

bull Use to provide evidence emphasis or efficiency

bull As with tables data in charts may be easier to understand than long statements of text

bull Figures with legends and abbreviations should be easily understandable

Figure Prep Rule of Thumb

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

bull Determine journalrsquos limitations on number size and types of figuresillustrations

bull Black and white vs colorbull Journal may not accept one or the other

bull Journal may charge for use of color

bull Resolution of images typical requirementsbull At least 300 dpi for photographs

bull 1200 dpi for line art

bull May require glossy prints (hard copies) or specific electronic file types

bull Label all figures according to journalrsquos specifications

bull Clearly mark the correct orientation of the photograph

bull Provide figure legends describing what is shown

Figure Preparation (Contrsquod)

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Anatomy of Figures (Graph)

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Anatomy of Figures (Chart)

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Graph Example Bar Chart

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Graph Example Frequency Histogram

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Graph Example X-Y Scatter Plot

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Graph Example X-Y Line Plot

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

ScatterLine Plot Curve Fitting

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Photograph Example SEM

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Photograph Example DC Picture

(Before charging) (Charging by PV)

(Fully charged) (Removal of PV)

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

0-35 AS

35-70 AS

70-100 AS

Done by TzengFEB 2005

1o Ammonium Sulfate Precipitation

2o Anion Exchange Chromatography

Photograph Example Protein PAGE

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Daytime application an IWECB-

based window can be charged and

colored by a photovoltaic cell

(1) to store solar energy and

(2) to attenuate solar irradiation

Nighttime application an IWECB-

based window can be discharged amp

bleached

(1) to drive low-power devices and

(2) to increase the illumination

Line Drawing Schematic Illustration

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Line Drawing Method and Theory

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Line Drawing Experimental Setup

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

01 1 10 100 1000

10x106

10x107

S = SO + S

MAX [A] (K

D + [A]) + k[A]

Ideal model for fitting total bindingK

D = 8 nM k = 7944 K

N ~ O (1 M)

Typ

hoo

n R

eadin

gAptamer No108 Conc (nM)

Surface for TP immobilization

Protein

Aptamer

Biotin

Streptavidin (SA)

Alkaline Phosphatase (AP) Unreacted

Substrate

ReactedSubstrate

Enzyme-Linked Immunosorbent Assay (ELISA)

SA-Biotin interaction Kd ~ 10-15 M = fM ~ ca 260 pNt

Typhoon scan aptamer 5-fold dilution series

Aptamer with nM affinity confirmed

Kd = 8 nM

Combination of Figure Presentation

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

Frequently Used Software

bull Word

bull PowerPoint

bull CorelDraw

bull Photoshop

bull Excel

bull Origin

bull Grapher

bull SigmaPlot

bull Othershellip

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

A final and essential step for data presentation consider the efficiency (do things right) and effectiveness (do the right things) Recall the model of ldquoApple Dailyrdquo

If you canrsquot read more papershellip

Can you tell the difference between the figures in a rejected paper and an accepted paper

LC Chen at BIME NTU

同學在論文圖表製作過程中常見違反學術倫理而不自覺的情事

直接剪貼網路（ Internet ）或文獻（ PDF ）圖片但未經授權甚至未合理引用 （ cf 原文引用）

製作漂亮數據或使用合成照片以滿足自己的結論

未重複實驗僅報導成功一次的數據

LC Chen at BIME NTU

Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES

httpwwwcartoonstockcomdirectorysscientistasp

LC Chen at BIME NTU

Essence of Scientific ResearchA valuable thesis is based on the REPRODUCIBLE FACTS AND FIGURES and the most importantly YOUR OWN CREACTIONS NOT NUMBER OF PAGES

httpwwwcartoonstockcomdirectorysscientistasp