Комплексные решения по переработке отходов древесины и биомассы
ЭНЗИМАТИЧЕСКИЕ МЕТОДЫ КОНВЕРСИИ БИОМАССЫ:...
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Transcript of ЭНЗИМАТИЧЕСКИЕ МЕТОДЫ КОНВЕРСИИ БИОМАССЫ:...
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EG-G. . () CBH
(C5 C6)
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10 FPU / 1 + 20 bGU / , [S] = 100 /, pH 5, 50C, 24 h / / / / / / / ()
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, -, () - : Trichoderma sp. Penicillium sp. Aspergillus sp.
: (CBH1, CBH2, Eg1, Eg2, Eg3, .)- (, -1,4/1,3-) (-, -, -, , -, -, .) (-, ) (-, , .) (-, -)
:Penicillium 537Penicillium PCA Aspergillus sp.
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(I) - , - ,
,
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. , 10 FPU / 1 , [S] = 5%, 5, 50 PenicilliumTrichoderma
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Penicillium Trichoderma
, Trichoderma
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Penicillium Trichoderma Penicillium , Trichoderma
: - ()
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-
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( , ) (, ) ( ) () , ( , ) (1-, 3- ), , ,
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(II) () -
-
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() (II)
- Penicillium 537 (CBH1 ) -1,4- Penicillium 537(CBH1 )
-GEg
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() - (II) -1,4- Penicillium 537 (CBH1 ) -1,4- Penicillium 537(CBH1 ) XylMan
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Penicillium 537 (II). 537-Cell. 537-Xyl. 537-bGl .537-Cell .537-Xyl .537-bGL .537-Cell .537-Xyl .537-bGl Cell + Xyl + bGl
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Penicillium 537 (II)
. 537-Cell
. 537-Xyl
. 537-bGl 537-Cell + 537-Xyl + 537-bGl
Cell + Xyl + bGl
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(III) ()
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() (III)
- Penicillium 537(Hist )
5 / []- 5%, 5.0, 50C
1 , 24 , -G
4
6.14987925.3047619048
7.246087.1037037037
7.8809287.55
2
: 50, 50 / S, 2 , 1000rpm, V=1,5
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1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1512.0100.84930.98B1-221-1512.0800.61566.15
pHist1--Glu12.0200.64851.97pHist1 cl.22.0800.56877.25
pHist4--Glu22.0200.6411.96pHist4 cl.32.0800.64657.88
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1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1512.050.47690.95B1-221-1512.0200.66845.30
pHist1--Glu12.0100.40311.92pHist1 cl.22.0400.38367.10
pHist4--Glu22.0100.39361.87pHist4 cl.32.0400.40777.55
: 50, 50 / S, 2 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
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1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1512.0200.78251.84B1-221-1512.01600.35648.57
pHist1--Glu12.0400.24372.30pHist1 cl.22.01600.25719.41
pHist4--Glu22.0400.20362.13pHist4 cl.32.01600.21458.71
F100.6100.47970.65F100.6800.22923.37
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1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1512.050.85961.71B1-221-1512.0400.43026.83
pHist1--Glu12.0100.42192.01pHist1 cl.22.0400.49039.08
pHist4--Glu22.0100.42672.03pHist4 cl.32.0400.45438.41
F10F100.6100.15640.63F100.6400.21183.39
2
[], /
[], /
5
1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 1
10
1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 24
: 50, 50 / S, 5 , 1000rpm, V=1,5
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1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-1515.0400.42462.395.01600.500910.65
pHist1 cl.215.0400.52613.4515.01600.494213.28
pHist4 cl.325.0400.5423.5125.01600.510213.54
pHist5 cl.335.0200.88132.4535.01600.390311.58
pHist6 cl.145.0400.37582.8445.01600.425212.15
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1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-1515.0100.44631.775.0400.57779.17
pHist1 cl.215.0100.65443.1215.0400.659312.21
pHist4 cl.325.0100.70813.3725.0400.696112.89
pHist5 cl.335.0100.38191.8235.0400.543510.06
pHist6 cl.145.0100.42912.0445.0400.57810.70
: 50, 50 / S, 5 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
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1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-1515.0400.64953.205.02000.550714.21
pHist1 cl.215.0400.58923.7115.02000.468516.07
pHist4 cl.325.0400.64953.9525.02000.461315.92
pHist5 cl.335.0400.66154.0035.02000.5116.92
pHist6 cl.145.0500.55264.4545.02000.588418.52
F101.5100.47970.65F101.5800.22923.37
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1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-1515.0100.73292.915.0800.375511.92
pHist1 cl.215.0200.37723.5915.0800.420515.57
pHist4 cl.325.0200.35883.4225.0800.425115.74
pHist5 cl.335.0200.37623.5835.0800.450116.67
pHist6 cl.145.0200.41713.9745.0800.489618.13
F10F101.5100.15640.63F101.5400.21183.39
00
00
00
00
00
1, 50/ S, 50, 5 / 1 S, 1
F10
00
00
00
00
00
00
1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 1
00
00
00
00
00
1, 50/ S, 50, 5 / 1 S, 24
00
00
00
00
00
00
1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 24
: 50, 50 / S, 10 , 1000rpm, V=1,5
-
1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-151110.0400.68113.3110.02000.658616.15
pHist1 cl.2210.0500.58454.61110.02000.660920.00
pHist4 cl.3310.0500.60514.71210.02000.576318.27
pHist5 cl.310.0400.56643.61310.02000.479716.30
pHist6 cl.1410.0400.68514.10410.02000.566918.08
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1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-151110.0200.35762.8410.0800.451814.34
pHist1 cl.2210.0200.45754.36110.0800.486818.03
pHist4 cl.3310.0200.48054.58210.0800.476217.64
pHist5 cl.310.0200.29852.84310.0800.422815.66
pHist6 cl.1410.0200.32473.09410.0800.471217.45
: 50, 50 / S, 10 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
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1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-15110.0800.47025.1010.02000.869819.95
pHist1 cl.2110.0800.3345.33110.02000.786122.55
pHist4 cl.3210.0800.34365.41210.02000.7280121.37
pHist5 cl.3310.0800.44666.25310.02000.908925.06
pHist6 cl.1410.0800.36655.60410.02000.897724.83
F103.0100.47970.65F103.0800.22923.37
-
1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-1518.3200.56334.4710.0800.587818.66
pHist1 cl.217.5200.54545.19110.0800.59622.07
pHist4 cl.325.0200.50324.79210.0800.550220.38
pHist5 cl.33200.55015.24310.0800.654224.23
pHist6 cl.147.9200.54185.16410.0800.641723.77
F10F103100.15640.63F103400.21183.39
00
00
00
00
00
1, 50/ S, 50, 10 / 1 S, 1
00
00
00
00
00
00
1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 1
00
00
00
00
00
1, 50/ S, 50, 10 / 1 S, 24
00
00
00
00
00
00
1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 24
C, mg/g C, mg/g 280pNPh-b-Glu, U/mgCMC, U/mgAvicel, U/mgCellobiose, U/mg
pHist1 cl.27037422.4221.090.624.06
pHist4 cl.35296874.4315.470.797.58
pHist5 cl.36256930.9712.990.590.73
pHist6 cl.15896531.214.810.740.79
, # 87.6
1: 2 1
-3 2448,
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l3
3-255.1.2B1-221-151400.34952.121000.5797.361200.56828.7124
pHist1 cl.2400.59113.711200.49719.991600.440412.4048
pHist4 cl.3400.63483.891200.521810.301600.454412.63
pHist5 cl.3400.27612.431200.3598.301600.358411.06
pHist6 cl.1400.39592.921200.42529.111600.414311.97
-10.37170.0510.37170.0510.33050.05
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151200.20671.58400.33415.12400.40466.20
pHist1 cl.2200.25262.17400.43957.55400.50518.67
pHist4 cl.3200.3042.61400.44267.60400.49138.43
pHist5 cl.3200.14251.22400.29695.10400.38556.62
pHist6 cl.1200.18081.55400.32345.55400.42327.27
-20.0360.0320.0340.0310.09260.04
1: 5 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-151800.35574.281600.733814.002000.585114.83
pHist1 cl.2800.51486.811600.50813.502000.445415.60
pHist4 cl.3800.57197.271600.563514.412000.422415.13
pHist5 cl.3800.31155.151600.43112.242000.393914.55
pHist6 cl.1800.46166.371600.516313.642000.45415.78
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151400.17412.67800.28058.60800.3049.32
pHist1 cl.2400.31975.49800.314210.79800.330811.36
pHist4 cl.3400.34385.90800.327611.25800.319810.98
pHist5 cl.3400.17453.00800.28729.86800.295510.15
pHist6 cl.1400.19573.36800.311710.70800.325311.17
1: 10 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-1511600.22896.743200.236713.703200.29715.43
pHist1 cl.21600.313810.332000.548317.702000.578618.32
pHist4 cl.31600.326110.532000.552117.782000.56818.10
pHist5 cl.3800.59447.462000.466116.022000.563118.00
pHist6 cl.1800.71928.472000.551817.772000.586318.47
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151800.16014.911600.169810.411600.184711.32
pHist1 cl.2400.46167.92800.400713.76800.396713.62
pHist4 cl.3400.47838.21800.388713.35800.389213.36
pHist5 cl.3400.28864.95800.364312.51800.393113.50
pHist6 cl.1400.32245.53800.38713.29800.39313.49
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00
00
00
00
00
00
, 2 , 3
00
00
00
00
00
00
, 2 , 24
00000
00000
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B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00
00
00
00
00
, 5 , 3
00
00
00
00
00
, 5 , 24
00000
00000
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B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00
00
00
00
00
, 10 , 3
00
00
00
00
00
, 10 , 24
00
00
00
00
00
00
, 2 , 48
00
00
00
00
00
, 5 , 48
00
00
00
00
00
, 10 , 48
, # 87.6
1: 2 1
-3 2448,
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l3
3-255.1.2B1-221-151400.62083.091180.63149.241180.69879.9524
pHist1 cl.2800.25124.661600.413810.541600.473711.4148
pHist4 cl.3800.21224.341600.30729.011600.457711.18
pHist5 cl.3800.20524.281600.379110.051600.425910.72
pHist6 cl.1800.30625.101600.462711.251600.463111.25
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151200.28912.22400.43746.73400.50017.66
pHist1 cl.2200.34892.99400.47538.16400.52198.96
pHist4 cl.3200.38123.27400.50998.75400.55679.56
pHist5 cl.3200.3693.17400.46237.94400.53779.23
pHist6 cl.1200.41913.60400.52449.00400.596210.24
1: 5 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-151800.70246.771600.816115.182000.69916.87
pHist1 cl.21000.44687.811600.566914.462000.444715.59
pHist4 cl.31000.41067.441600.579214.662000.43915.47
pHist5 cl.31000.41547.491600.629715.492000.484216.39
pHist6 cl.11000.52878.651600.705516.722000.51717.06
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151400.30884.75800.31019.54800.351610.78
pHist1 cl.2400.37916.51800.344711.84800.374512.86
pHist4 cl.3400.35396.08800.333511.45800.345211.85
pHist5 cl.3400.35586.11800.367412.61800.36712.60
pHist6 cl.1400.41687.16800.380213.05800.379113.02
1: 10 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-1511600.58111.803200.321716.143200.348216.91
pHist1 cl.21600.364411.162000.597518.702000.620919.18
pHist4 cl.31600.349310.912000.510516.932000.52617.24
pHist5 cl.31600.371911.282000.591318.582000.641419.60
pHist6 cl.11600.401711.772000.642219.612000.642819.63
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151800.23027.08800.366711.28800.384611.79
pHist1 cl.2400.50468.66800.401913.80800.420514.44
pHist4 cl.3400.49068.42800.361512.41800.374513.50
pHist5 cl.3400.50668.70800.410114.08800.441115.90
pHist6 cl.1400.57189.82800.428314.71800.428415.44
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
F10 3-194.1.20,6 / 1 S100.10420.31100.68080.83200.50441.34
F10 3-194.1.21,7 / 1 S100.41750.59200.76341.80400.57582.93
F10 3-194.1.23 / 1 S200.33021.02400.64173.17800.44284.91
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
F10 3-194.1.20,6 / 1 S40.15050.2350.37930.73100.27141.04
F10 3-194.1.21,7 / 1 S50.2190.4250.80731.55100.55852.14
F10 3-194.1.23 / 1 S100.17540.67100.69942.69200.46093.53
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00
00
00
00
00
, 2 , 3
00
00
00
00
00
, 2 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00
00
00
00
00
, 5 , 3
00
00
00
00
00
, 5 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00
00
00
00
00
, 10 , 3
00
00
00
00
00
, 10 , 24
00
00
00
00
00
, 2 , 48
00
00
00
00
00
, 5 , 48
00
00
00
00
00
, 10 , 48
000
000
000
0,6 / 1 S
1,7 / 1 S
3 /1 S
,
[], /
000
000
000
0,6 / 1 S
1,7 / 1 S
3 /1 S
,
[], /
00
00
00
[], /
, 3
00
00
00
[], /
, 24
00
00
00
[], /
, 48
7
10.64991369.1698412698
13.2763212.2092592593
13.5374412.8907407407
2
: 50, 50 / S, 2 , 1000rpm, V=1,5
-
1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1512.0100.84930.98B1-221-1512.0800.61566.15
pHist1--Glu12.0200.64851.97pHist1 cl.22.0800.56877.25
pHist4--Glu22.0200.6411.96pHist4 cl.32.0800.64657.88
-
1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1512.050.47690.95B1-221-1512.0200.66845.30
pHist1--Glu12.0100.40311.92pHist1 cl.22.0400.38367.10
pHist4--Glu22.0100.39361.87pHist4 cl.32.0400.40777.55
: 50, 50 / S, 2 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
-
1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1512.0200.78251.84B1-221-1512.01600.35648.57
pHist1--Glu12.0400.24372.30pHist1 cl.22.01600.25719.41
pHist4--Glu22.0400.20362.13pHist4 cl.32.01600.21458.71
F100.6100.47970.65F100.6800.22923.37
-
1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1512.050.85961.71B1-221-1512.0400.43026.83
pHist1--Glu12.0100.42192.01pHist1 cl.22.0400.49039.08
pHist4--Glu22.0100.42672.03pHist4 cl.32.0400.45438.41
F10F100.6100.15640.63F100.6400.21183.39
2
[], /
[], /
5
1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 1
10
1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 24
: 50, 50 / S, 5 , 1000rpm, V=1,5
-
1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1515.0400.42462.395.01600.500910.65
pHist1--Glu15.0400.52613.4515.01600.494213.28
pHist4--Glu25.0400.5423.5125.01600.510213.54
-
1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1515.0100.44631.775.0400.57779.17
pHist1--Glu15.0100.65443.1215.0400.659312.21
pHist4--Glu25.0100.70813.3725.0400.696112.89
: 50, 50 / S, 5 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
-
1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2P.verr 221-1515.0400.64953.205.02000.550714.21
pHist1--Glu15.0400.58923.7115.02000.468516.07
pHist4--Glu25.0400.64953.9525.02000.461315.92
F101.5100.47970.65F101.5800.22923.37
-
1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2P.verr 221-1515.0100.73292.915.0800.375511.92
pHist1--Glu15.0200.37723.5915.0800.420515.57
pHist4--Glu25.0200.35883.4225.0800.425115.74
F10F101.5100.15640.63F101.5400.21183.39
P.verr 221-151P.verr 221-151
pHist1--GlupHist1--Glu
pHist4--GlupHist4--Glu
F10
P.verr 221-151P.verr 221-151
pHist1--GlupHist1--Glu
pHist4--GlupHist4--Glu
F10F10
1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 1
P.verr 221-151P.verr 221-151
pHist1--GlupHist1--Glu
pHist4--GlupHist4--Glu
P.verr 221-151P.verr 221-151
pHist1--GlupHist1--Glu
pHist4--GlupHist4--Glu
F10F10
1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 24
: 50, 50 / S, 10 , 1000rpm, V=1,5
-
1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-151110.0400.68113.3110.02000.658616.15
pHist1 cl.2210.0500.58454.61110.02000.660920.00
pHist4 cl.3310.0500.60514.71210.02000.576318.27
pHist5 cl.310.0400.56643.61310.02000.479716.30
pHist6 cl.1410.0400.68514.10410.02000.566918.08
-
1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-151110.0200.35762.8410.0800.451814.34
pHist1 cl.2210.0200.45754.36110.0800.486818.03
pHist4 cl.3310.0200.48054.58210.0800.476217.64
pHist5 cl.310.0200.29852.84310.0800.422815.66
pHist6 cl.1410.0200.32473.09410.0800.471217.45
: 50, 50 / S, 10 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)
-
1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l
3-255.1.2B1-221-15110.0800.47025.1010.02000.869819.95
pHist1 cl.2110.0800.3345.33110.02000.786122.55
pHist4 cl.3210.0800.34365.41210.02000.7280121.37
pHist5 cl.3310.0800.44666.25310.02000.908925.06
pHist6 cl.1410.0800.36655.60410.02000.897724.83
F103.0100.47970.65F103.0800.22923.37
-
1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l
3-255.1.2B1-221-1518.3200.56334.4710.0800.587818.66
pHist1 cl.217.5200.54545.19110.0800.59622.07
pHist4 cl.325.0200.50324.79210.0800.550220.38
pHist5 cl.33200.55015.24310.0800.654224.23
pHist6 cl.147.9200.54185.16410.0800.641723.77
F10F103100.15640.63F103400.21183.39
00
00
00
00
00
1, 50/ S, 50, 10 / 1 S, 1
00
00
00
00
00
00
1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 1
00
00
00
00
00
1, 50/ S, 50, 10 / 1 S, 24
00
00
00
00
00
00
1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 24
C, mg/g C, mg/g 280pNPh-b-Glu, U/mgCMC, U/mgAvicel, U/mgCellobiose, U/mg
pHist1 cl.27037422.4221.090.624.06
pHist4 cl.35296874.4315.470.797.58
pHist5 cl.36256930.9712.990.590.73
pHist6 cl.15896531.214.810.740.79
, # 87.6
1: 2 1
-3 2448,
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l3
3-255.1.2B1-221-151400.34952.121000.5797.361200.56828.7124
pHist1 cl.2400.59113.711200.49719.991600.440412.4048
pHist4 cl.3400.63483.891200.521810.301600.454412.63
pHist5 cl.3400.27612.431200.3598.301600.358411.06
pHist6 cl.1400.39592.921200.42529.111600.414311.97
-10.37170.0510.37170.0510.33050.05
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151200.20671.58400.33415.12400.40466.20
pHist1 cl.2200.25262.17400.43957.55400.50518.67
pHist4 cl.3200.3042.61400.44267.60400.49138.43
pHist5 cl.3200.14251.22400.29695.10400.38556.62
pHist6 cl.1200.18081.55400.32345.55400.42327.27
-20.0360.0320.0340.0310.09260.04
1: 5 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-151800.35574.281600.733814.002000.585114.83
pHist1 cl.2800.51486.811600.50813.502000.445415.60
pHist4 cl.3800.57197.271600.563514.412000.422415.13
pHist5 cl.3800.31155.151600.43112.242000.393914.55
pHist6 cl.1800.46166.371600.516313.642000.45415.78
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151400.17412.67800.28058.60800.3049.32
pHist1 cl.2400.31975.49800.314210.79800.330811.36
pHist4 cl.3400.34385.90800.327611.25800.319810.98
pHist5 cl.3400.17453.00800.28729.86800.295510.15
pHist6 cl.1400.19573.36800.311710.70800.325311.17
1: 10 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-1511600.22896.743200.236713.703200.29715.43
pHist1 cl.21600.313810.332000.548317.702000.578618.32
pHist4 cl.31600.326110.532000.552117.782000.56818.10
pHist5 cl.3800.59447.462000.466116.022000.563118.00
pHist6 cl.1800.71928.472000.551817.772000.586318.47
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151800.16014.911600.169810.411600.184711.32
pHist1 cl.2400.46167.92800.400713.76800.396713.62
pHist4 cl.3400.47838.21800.388713.35800.389213.36
pHist5 cl.3400.28864.95800.364312.51800.393113.50
pHist6 cl.1400.32245.53800.38713.29800.39313.49
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00
00
00
00
00
00
, 2 , 3
00
00
00
00
00
00
, 2 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00
00
00
00
00
, 5 , 3
00
00
00
00
00
, 5 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00
00
00
00
00
, 10 , 3
00
00
00
00
00
, 10 , 24
00
00
00
00
00
00
, 2 , 48
00
00
00
00
00
, 5 , 48
00
00
00
00
00
, 10 , 48
, # 87.6
1: 2 1
-3 2448,
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l3
3-255.1.2B1-221-151400.62083.091180.63149.241180.69879.9524
pHist1 cl.2800.25124.661600.413810.541600.473711.4148
pHist4 cl.3800.21224.341600.30729.011600.457711.18
pHist5 cl.3800.20524.281600.379110.051600.425910.72
pHist6 cl.1800.30625.101600.462711.251600.463111.25
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151200.28912.22400.43746.73400.50017.66
pHist1 cl.2200.34892.99400.47538.16400.52198.96
pHist4 cl.3200.38123.27400.50998.75400.55679.56
pHist5 cl.3200.3693.17400.46237.94400.53779.23
pHist6 cl.1200.41913.60400.52449.00400.596210.24
1: 5 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-151800.70246.771600.816115.182000.69916.87
pHist1 cl.21000.44687.811600.566914.462000.444715.59
pHist4 cl.31000.41067.441600.579214.662000.43915.47
pHist5 cl.31000.41547.491600.629715.492000.484216.39
pHist6 cl.11000.52878.651600.705516.722000.51717.06
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151400.30884.75800.31019.54800.351610.78
pHist1 cl.2400.37916.51800.344711.84800.374512.86
pHist4 cl.3400.35396.08800.333511.45800.345211.85
pHist5 cl.3400.35586.11800.367412.61800.36712.60
pHist6 cl.1400.41687.16800.380213.05800.379113.02
1: 10 1
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
3-255.1.2B1-221-1511600.58111.803200.321716.143200.348216.91
pHist1 cl.21600.364411.162000.597518.702000.620919.18
pHist4 cl.31600.349310.912000.510516.932000.52617.24
pHist5 cl.31600.371911.282000.591318.582000.641419.60
pHist6 cl.11600.401711.772000.642219.612000.642819.63
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
3-255.1.2B1-221-151800.23027.08800.366711.28800.384611.79
pHist1 cl.2400.50468.66800.401913.80800.420514.44
pHist4 cl.3400.49068.42800.361512.41800.374513.50
pHist5 cl.3400.50668.70800.410114.08800.441115.90
pHist6 cl.1400.57189.82800.428314.71800.428415.44
-3 24 48
RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l
F10 3-194.1.20,6 / 1 S100.10420.31100.68080.83200.50441.34
F10 3-194.1.21,7 / 1 S100.41750.59200.76341.80400.57582.93
F10 3-194.1.23 / 1 S200.33021.02400.64173.17800.44284.91
-
RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l
F10 3-194.1.20,6 / 1 S40.15050.2350.37930.73100.27141.04
F10 3-194.1.21,7 / 1 S50.2190.4250.80731.55100.55852.14
F10 3-194.1.23 / 1 S100.17540.67100.69942.69200.46093.53
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 2 1
00
00
00
00
00
, 2 , 3
00
00
00
00
00
, 2 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 5 1
00
00
00
00
00
, 5 , 3
00
00
00
00
00
, 5 , 24
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00000
00000
00000
B1-221-151
pHist1 cl.2
pHist4 cl.3
pHist5 cl.3
pHist6 cl.1
,
[], /
, 10 1
00
00
00
00
00
, 10 , 3
00
00
00
00
00
, 10 , 24
00
00
00
00
00
, 2 , 48
00
00
00
00
00
, 5 , 48
00
00
00
00
00
, 10 , 48
000
000
000
0,6 / 1 S
1,7 / 1 S
3 /1 S
,
[], /
000
000
000
0,6 / 1 S
1,7 / 1 S
3 /1 S
,
[], /
00
00
00
[], /
, 3
00
00
00
[], /
, 24
00
00
00
[], /
, 48
-
(IV) - ()
-
(IV) , pH5, 50, 72 0.2 /, 0.1 /Penicillium eTrichoderma :Spezyme CP,Multifect XL,CP + XL
Chart2
3.652173913
2.8695652174
1.6956521739
1.8260869565
2.1739130435
2.2608695652
2.17
1.64
0.98
0.3826086957
11.0909090909
4.4545454545
3.0909090909
1.5454545455
1.4090909091
1.08
1.1363636364
0.6363636364
0.2727272727
7.2727272727
1.1818181818
7.33
0.2434782609
Glucose (g/L)
Exp 1
Substate: Pretreated corn cob
Moisture of substrate: 65 %.
Yield ofYield of
1 h6 h244872016244872glucoseglucosePeakCglu, g/l
C. lucknowenseC. lucknowense
2C1 CBH Ia 650.50.260.650.50.37C1 CBH Ia 650.200.351.062.02.875.214.958542.8
3C1 CBH Ib 600.450.210.450.380.3C1 CBH Ib 600.170.250.691.52.264.111.762733.0
4C1 CBH IIb 700.570.310.640.410.28C1 CBH IIb 700.230.441.041.62.093.810.855752.6
5C1 EG II 510.640.360.750.490.34C1 EG II 510.260.531.242.02.614.713.678404.0
6C1 EG V 250.510.280.440.270.36C1 EG V 250.200.380.681.01.392.57.264283.1
7C1 EG VI 470.470.250.500.330.43C1 EG VI 470.180.330.791.31.703.18.855292.6
8C1 Xyl II 240.630.160.270.160.2C1 Xyl II 240.130.160.360.50.701.33.6
9C1 b-Glu 1060.820.230.300.190.23C1 b-Glu 1060.170.290.430.70.831.54.361373.0
T. reesei0.07T. reesei
10A1 CBH I 660.560.361.020.650.46A1 CBH I 660.220.531.732.73.656.619.0108655.7
11A1 CBH I 570.510.240.530.450.37A1 CBH I 570.200.310.841.82.875.214.963823.1
12A1 CBH II 600.60.330.590.350.43A1 CBH II 600.240.470.941.31.703.18.8
13A1 CBH II 500.670.350.610.40.25A1 CBH II 500.270.510.981.61.833.39.5
14A1 EG I hm0.540.330.710.440.29A1 EG I hm0.210.471.161.72.174.011.3
15A1 EG I lm0.720.410.730.430.3A1 EG I lm0.300.621.191.72.264.111.7
16A1 EG II hm0.70.340.600.350.22A1 EG II hm0.290.490.961.31.572.88.1
17A1 EG II lm0.590.30.450.240.16A1 EG II lm0.240.420.680.91.041.95.4
18A1 Xyl II 210.940.250.420.250.28A1 Xyl II 210.200.330.640.91.041.95.4
19substrate0.570.140.150.090.11substrate0.110.130.150.20.240.41.311090.0
20b-glu A. j.0.770.170.230.120.15b-glu A. j.0.160.180.290.30.380.72.0
14410
0.5
Yield ofYield of
16 h244872016244872sugarssugars
C. lucknowenseC. lucknowense% of dry S
2C1 CBH Ia 650.420.130.660.80.58C1 CBH Ia 650.270.301.472.13.25.88.899184.4045
3C1 CBH Ib 600.380.120.310.460.5C1 CBH Ib 600.250.290.901.42.95.27.9
4C1 CBH IIb 700.970.210.440.780.94C1 CBH IIb 700.490.371.112.12.34.26.4
5C1 EG II 511.60.420.980.470.7C1 EG II 510.750.541.992.93.76.710.1
6C1 EG V 2520.720.781.120.60C1 EG V 250.910.781.672.83.35.99.0
7C1 EG VI 471.110.20.440.61C1 EG VI 470.550.361.111.72.44.56.7
8C1 Xyl II 243.923.143.140.440.6C1 Xyl II 241.702.765.526.98.214.822.5
9C1 b-Glu 1060.660.250.280.400.50C1 b-Glu 1060.370.400.851.31.42.63.9
T. reeseiT. reesei
10A1 CBH I 660.870.31.220.710.91A1 CBH I 660.450.442.383.94.58.212.5
11A1 CBH I 570.430.110.470.280.93A1 CBH I 570.270.291.162.12.34.26.4
12A1 CBH II 600.60.10.273.061.3A1 CBH II 600.340.280.831.32.13.75.7
13A1 CBH II 500.880.110.360.620.95A1 CBH II 500.460.290.981.82.34.36.5
14A1 EG I hm1.050.331.183.020.7A1 EG I hm0.530.472.323.33.76.710.1
15A1 EG I lm1.2310.82.480.78A1 EG I lm0.601.011.702.84.07.311.0
16A1 EG II hm1.040.60.71.570.94A1 EG II hm0.520.691.531.82.34.26.4
17A1 EG II lm0.140.110.06511.2A1 EG II lm0.160.290.500.50.61.01.6
18A1 Xyl II 213.763.13.281.40.67A1 Xyl II 211.632.735.756.77.112.919.62.2805555556
19substrate0.120.10.0360.430.27substrate0.150.280.310.30.30.50.7
20b-glu A. j.0.140.110.030.780.86b-glu A. j.0.160.290.440.40.61.01.6
48162020
C. lucknowense
CBH Ia 651.40.91.41.11.1
CBH Ib 601.51.21.31.01.3
CBH Iib 702.20.81.11.31.1
EG II 512.91.01.61.51.4
EG V 254.62.12.52.82.3
EG VI 473.01.11.41.41.4
Xyl II 2413.316.915.213.311.7
b-Glu 1062.21.42.02.01.7
T. reesei
CBH I hm2.00.81.41.51.2
CBH I lm1.40.91.41.20.8
CBH II hm1.40.60.91.01.2
CBH II lm1.70.61.01.11.3
EG I hm2.51.02.01.91.7
EG I lm2.01.61.41.61.8
EG II hm1.81.41.61.41.5
EG II lm0.70.70.70.60.5
Xyl II8.38.39.07.36.8
substrate1.32.22.11.31.1
b-glu A. j.1.01.61.51.21.5
Exp 1
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
C1 CBH Ia 65
C1 CBH Ib 60
C1 CBH IIb 70
C1 EG II 51
C1 EG V 25
C1 EG VI 47
C1 Xyl II 24
C1 b-Glu 106
A1 CBH I 66
A1 CBH I 57
A1 CBH II 60
A1 CBH II 50
A1 EG I hm
A1 EG I lm
A1 EG II hm
A1 EG II lm
A1 Xyl II 21
substrate
b-glu A. j.
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
Exp 2
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
C1 CBH Ia 65
C1 CBH Ib 60
C1 CBH IIb 70
C1 EG II 51
C1 EG V 25
C1 EG VI 47
C1 Xyl II 24
C1 b-Glu 106
A1 CBH I 66
A1 CBH I 57
A1 CBH II 60
A1 CBH II 50
A1 EG I hm
A1 EG I lm
A1 EG II hm
A1 EG II lm
A1 Xyl II 21
substrate
b-glu A. j.
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
Total Bars
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
Cglu, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 6 hours
Total kinetics
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
72
Cglu, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 72 hours
Xyl II
Xyl II
b-Glu C1
b-glu A. j.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
CRS, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 6 hours
b-glu A. j.
b-Glu C1
Xyl II
Xyl II
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
CRS, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 72 hours
Xyl II
Xyl II
b-Glu C1
b-glu A. j.
Substate: Pretreated corn cob
Moisture of substrate: 65 %.
Yield ofYield of
1 h6 h244872016244872glucoseglucose
P. verruculosumP. verruculosum
1B1 CBH Ia 660.521.122.31.121.29B1 CBH Ia 660.411.915.079.411.0920.265.0
2B1 CBH I 550.340.490.820.420.56B1 CBH I 550.250.761.703.34.458.126.1
3B1 CBH IIb 600.330.460.820.320.41B1 CBH IIb 600.240.711.702.43.095.618.1
4B1 EG I 700.290.270.460.330.41B1 EG I 700.200.360.891.31.552.89.1
5B1 EG I 520.30.270.390.290.38B1 EG I 520.210.360.731.11.412.68.3
6B1 EG II 390.240.210.290.180.36B1 EG II 390.150.250.500.60.661.23.9
7B1 b-Glu 1060.460.851.650.770.91B1 b-Glu 1060.351.423.596.37.6413.944.8
P. canescensP. canescens
8a-L-AF 700.170.150.220.120.22a-L-AF 700.090.150.340.30.340.62.0
9a-L-AF 600.120.110.170.0950.18a-L-AF 600.050.070.230.20.250.51.5
10Xyl 10 A0.20.190.260.1450.27Xyl 10 A0.120.220.430.50.450.82.7
11a-Gal A0.170.140.220.120.23a-Gal A0.090.130.340.30.360.72.1
12C1 Ara 300.130.110.170.0950.17C1 Ara 300.050.070.230.20.230.41.3
13Pec0.150.120.200.120.22Pec0.070.090.300.30.340.62.0
14C1 EG III0.440.390.640.440.54C1 EG III0.340.581.301.72.143.912.5
15C1 Xyl II 240.180.170.270.180.34C1 Xyl II 240.100.180.450.60.611.13.6
16A1 Xyl II 210.270.250.380.250.27A1 Xyl II 210.180.330.700.90.911.75.3
17Spezyme 0,1 g/l0.490.781.600.730.87Spezyme 0,1 g/l0.381.293.486.07.2713.242.7
18Multifect 0,1 g/l0.280.310.470.170.33Multifect 0,1 g/l0.190.440.911.11.182.16.9
19S 0,07 g/l + M 0,025 g/l0.390.581.300.640.79S 0,07 g/l + M 0,025 g/l0.290.932.805.26.5511.938.4
24520
Yield ofYield of
1 h6 h244872016244872sugarssugars
P. verruculosumP. verruculosum% of dry S
1B1 CBH Ia 662.463.426.82.723.1B1 CBH Ia 661.093.007.3112.313.6424.837.6
2B1 CBH I 552.0623.181.131.32B1 CBH I 550.931.803.495.66.1511.216.9
3B1 CBH IIb 600.70.911.671.051.42B1 CBH IIb 600.350.881.912.63.286.09.0
4B1 EG I 700.650.591.170.751.04B1 EG I 700.330.611.382.02.484.56.8
5B1 EG I 520.720.50.910.700.85B1 EG I 520.360.541.111.92.083.85.7
6B1 EG II 390.30.10.150.660.34B1 EG II 390.190.200.310.50.510.91.4
7B1 b-Glu 1062.422.965.281.502.06B1 b-Glu 1061.082.615.718.99.2616.825.5
P. canescensP. canescens
8a-L-AF 700.310.270.190.570.3a-L-AF 700.190.350.350.40.460.81.3
9a-L-AF 600.10.090.100.450.22a-L-AF 600.100.190.250.30.380.71.0
10Xyl 10 A0.290.470.651.151.28Xyl 10 A0.180.510.831.41.492.74.1
11a-Gal A0.130.110.120.440.17a-Gal A0.110.210.270.30.330.60.9
12C1 Ara 302.11.482.960.982.3C1 Ara 300.941.363.265.05.149.314.2
13Pec0.120.070.110.330.43Pec0.110.180.260.30.300.50.8
14C1 EG III1.261.111.440.851.03C1 EG III0.591.051.662.22.464.56.8
15C1 Xyl II 243.844.084.901.341.59C1 Xyl II 241.683.555.316.57.2813.220.1
16A1 Xyl II 213.643.023.861.081.34A1 Xyl II 211.592.664.215.46.2311.317.2
17Spezyme 0,1 g/l3.63.887.042.562.82Spezyme 0,1 g/l1.573.397.5611.612.4622.734.3
18Multifect 0,1 g/l3.83.645.961.792.3Multifect 0,1 g/l1.663.186.428.410.2718.728.3
19S 0,07 g/l + M 0,025 g/l3.763.966.962.583.06S 0,07 g/l + M 0,025 g/l1.643.457.4711.713.4724.537.1
481020
P. verruculosum
CBH Ia 662.71.61.41.31.2
CBH I 553.82.42.11.71.4
CBH IIb 601.51.21.11.11.1
EG I 701.71.71.61.61.6
EG I 521.71.51.51.71.5
EG II 391.20.80.60.70.8
b-Glu 1063.01.81.61.41.2
P. canescens
a-L-AF 702.12.41.01.21.4
a-L-AF 602.22.71.11.41.5
Xyl 10 A1.52.41.93.13.3
a-Gal A1.31.70.81.00.9
Ara 30 C117.318.814.420.822.6
Pec1.51.90.90.80.9
EG III C11.81.81.31.31.2
Xyl II C116.819.511.710.711.9
Xyl II A18.88.16.05.96.9
Spezyme 0,1 g/l4.12.62.21.91.7
Multifect 0,1 g/l8.77.37.17.48.7
S 0,07 g/l + M 0,025 g/l5.63.72.72.22.1
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
B1 CBH Ia 66
B1 CBH I 55
B1 CBH IIb 60
B1 EG I 70
B1 EG I 52
B1 EG II 39
B1 b-Glu 106
a-L-AF 70
a-L-AF 60
Xyl 10 A
a-Gal A
C1 Ara 30
Pec
C1 EG III
C1 Xyl II 24
A1 Xyl II 21
Spezyme 0,1 g/l
Multifect 0,1 g/l
S 0,07 g/l + M 0,025 g/l
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
Cglu, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 6 hours
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
CRS, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 6 hours
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
0000000000000000000
B1 CBH Ia 66
B1 CBH I 55
B1 CBH IIb 60
B1 EG I 70
B1 EG I 52
B1 EG II 39
B1 b-Glu 106
a-L-AF 70
a-L-AF 60
Xyl 10 A
a-Gal A
C1 Ara 30
Pec
C1 EG III
C1 Xyl II 24
A1 Xyl II 21
Spezyme 0,1 g/l
Multifect 0,1 g/l
S 0,07 g/l + M 0,025 g/l
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
Cglu, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 72 hours
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6 h
CRS, g/l
Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 72 hours
Cglu, g/lCglu, g/l
6 h72 h
Tr CBH I 660.533.65
Tr CBH I 570.312.87
Tr CBH II 600.471.70
Tr CBH II 500.511.83
Tr EG I hm0.472.17
Tr EG I lm0.622.26
Tr EG II hm0.672.17
Tr EG II lm0.571.64
Tr Xyl II 210.330.98
A.j. BGL0.180.38
Pv CBH I 66 (A280)1.9111.09
Pv CBH I 550.764.45
Pv CBH II 600.713.09
Pv EG I 700.361.55
Pv EG I 520.361.41
Pv EG II 390.371.08
Pv EG III 250.671.14
Pv BGL 1160.470.64
Pv Xyl 230.220.27
Spezyme 0,1 g/l1.297.27
Multifect 0,1 g/l0.441.18
S 0,07 g/l + M 0,025 g/l1.157.33
Background substrate0.130.24
RS - Glu (g/l)RS - Glu (%)
6 h72 h72 h72 h
C1 CBH Ia 650.533.343.34100
C1 CBH Ib 600.362.632.63100
C1 CBH IIb 700.602.752.75100
C1 EG II 510.543.673.67100
C1 EG III 281.052.462.46100
C1 EG V 250.783.273.27100
C1 EG VI 470.362.452.45100
C1 Xyl II 243.167.727.72100
C1 AraGal 302.065.685.68100
C1 BGL 1060.401.431.43100
A1 CBH I 660.444.530.8819
A1 CBH I 570.292.31-0.56-24
A1 CBH II 600.282.050.3617
A1 CBH II 500.292.350.5222
A1 EG I hm0.473.671.5041
A1 EG I lm1.014.001.7443
A1 EG II hm0.662.850.6824
A1 EG II lm0.331.52-0.12-8
A1 Xyl II 212.706.675.6985
A.j. BGL0.290.580.1934
B1 CBH I 66 (A280)3.0013.642.5519
B1 CBH I 551.806.151.6928
B1 CBH II 600.883.280.196
B1 EG I 700.612.480.9438
B1 EG I 520.542.080.6832
B1 EG II 390.421.270.1915
B1 EG III 250.631.00-0.14-14
B1 BGL 1160.440.63-0.00-1
B1 Xyl 232.863.933.6693
P.c. AF 700.350.460.46100
P.c. AF 600.190.380.38100
P.c. a-Gal0.210.330.33100
P.c. Pec0.180.300.30100
Spezyme 0,1 g/l3.3912.465.1942
Multifect 0,1 g/l3.1810.279.0988
S 0,07 g/l + M 0,025 g/l4.5014.086.7548
Background substrate0.280.260.015
Glucose (g/L)
Reducing sugars (g/L)
Hydrolysis of pretreated corn cobs for 72 hours.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 U/ml, 1 mM NaN3, pH 5, 50 0.
00000000000
0.19545454550.17272727270.22727272730.25909090910.20.18181818180.33636363640.12727272730.10.17045454550.0545454545
0.34545454550.25454545450.43636363640.52727272730.38181818180.32727272730.58181818180.16363636360.18181818180.29090909090.0727272727
1.05612648220.69249011861.03913043481.23517786560.67549407110.79011857711.29545454550.36403162060.45454545450.42687747040.2272727273
21.47826086961.60869565221.956521739111.26086956521.73913043480.52173913040.60869565220.6521739130.2391304348
2.86956521742.26086956522.08695652172.60869565221.39130434781.69565217392.13636363640.69565217390.61363636360.82608695650.2272727273
C1 CBH Ia 65
C1 CBH Ib 60
C1 CBH IIb 70
C1 EG II 51
C1 EG V 25
C1 EG VI 47
C1 EG III
C1 Xyl II 24
C1 Xyl II 24
C1 b-Glu 106
C1 Ara 30
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0000000
0.40909090910.24545454550.23636363640.20.20909090910.15454545450.3545454545
1.90909090910.76363636360.70909090910.36363636360.36363636360.25454545451.4181818182
5.06818181821.70454545451.70454545450.88636363640.72727272730.53.5909090909
9.39130434783.30434782612.43478260871.26086956521.08695652170.60869565226.347826087
11.09090909094.45454545453.09090909091.54545454551.40909090910.65909090917.6363636364
B1 CBH Ia 66
B1 CBH I 55
B1 CBH IIb 60
B1 EG I 70
B1 EG I 52
B1 EG II 39
B1 b-Glu 106
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
00000
0.11363636360.15909090910.38181818180.19090909090.2909090909
0.12727272730.18181818181.29090909090.43636363640.9272727273
0.14545454550.29288537553.47727272730.90909090912.7954545455
0.21739130430.34782608761.13043478265.2173913043
0.24347826090.38260869577.27272727271.18181818186.5454545455
substrate
b-glu A. j.
Spezyme 0,1 g/l
Multifect 0,1 g/l
S 0,07 g/l + M 0,025 g/l
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0000000000
0.22272727270.20.24090909090.27272727270.21363636360.29545454550.28636363640.23636363640.19772727270.1818181818
0.52727272730.30909090910.47272727270.50909090910.47272727270.61818181820.49090909090.41818181820.32727272730.3272727273
1.73162055340.84387351780.94071146250.98181818181.16324110671.19090909090.95810276680.68260869570.63833992090.7045454545
2.6521739131.78260869571.3478260871.56521739131.73913043481.69565217391.3478260870.86956521740.91304347830.9130434783
3.6521739132.86956521741.69565217391.82608695652.17391304352.26086956521.56521739131.04347826091.04347826090.9090909091
A1 CBH I 66
A1 CBH I 57
A1 CBH II 60
A1 CBH II 50
A1 EG I hm
A1 EG I lm
A1 EG II hm
A1 EG II lm
A1 Xyl II 21
A1 Xyl II 21
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
00000
0.09090909090.04545454550.11818181820.09090909090.0727272727
0.14545454550.07272727270.21818181820.12727272730.0909090909
0.34090909090.22727272730.43181818180.34090909090.2954545455
0.3478260870.23913043480.45652173910.3478260870.347826087
0.34090909090.250.45454545450.36363636360.3409090909
a-L-AF 70
a-L-AF 60
Xyl 10 A
a-Gal A
Pec
t, h
Cglu, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
00000000000
0.26938775510.25306122450.4938775510.75102040820.91428571430.55102040820.58947368421.69795918371.67578947370.36734693880.9431578947
0.30204081630.2938775510.36734693880.53877551020.78367346940.35918367351.05263157892.75918367353.55368421050.41.3642105263
1.46938775510.89795918371.11020408161.99183673471.66530612241.11020408161.66315789475.51836734695.30526315790.84897959183.2631578947
2.12244897961.42857142862.08163265312.89795918372.77551020411.71428571432.21052631586.93877551026.48421052631.3061224494.9684210526
3.18367346942.85714285712.32653061223.67346938783.26530612242.44897959182.46315789478.16326530617.28421052631.42857142865.1368421053
C1 CBH Ia 65
C1 CBH Ib 60
C1 CBH IIb 70
C1 EG II 51
C1 EG V 25
C1 EG VI 47
C1 EG III
C1 Xyl II 24
C1 Xyl II 24
C1 b-Glu 106
C1 Ara 30
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0000000
1.09473684210.92631578950.35368421050.33263157890.36210526320.18526315791.0778947368
2.99789473681.80210526320.88421052630.61473684210.53894736840.20210526322.6105263158
7.30526315793.49473684211.90526315791.37894736841.10526315790.30526315795.7052631579
12.29473684215.62.631578947421.89473684210.45263157898.9473684211
13.64210526326.14736842113.28421052632.48421052632.08421052630.50526315799.2631578947
B1 CBH Ia 66
B1 CBH I 55
B1 CBH IIb 60
B1 EG I 70
B1 EG I 52
B1 EG II 39
B1 b-Glu 106
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
0000000000
0.45306122450.27346938780.34285714290.45714285710.52653061220.60.52244897960.15510204081.63265306121.5915789474
0.44081632650.28571428570.27755102040.28571428570.46530612241.0122448980.68571428570.28571428572.72653061222.6610526316
2.38367346941.15918367350.83265306120.97959183672.31836734691.69795918371.53469387760.49795918375.74693877554.2105263158
3.87755102042.12244897961.34693877551.75510204083.32653061222.77551020411.84693877550.5061224496.6938775515.3894736842
4.53061224492.3061224492.05102040822.34693877553.673469387842.32653061220.57142857147.10204081636.2315789474
A1 CBH I 66
A1 CBH I 57
A1 CBH II 60
A1 CBH II 50
A1 EG I hm
A1 EG I lm
A1 EG II hm
A1 EG II lm
A1 Xyl II 21
A1 Xyl II 21
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
00000
0.18947368420.10105263160.18105263160.11368421050.1094736842
0.34526315790.19368421050.51368421050.21052631580.1768421053
0.34736842110.25263157890.83157894740.27368421050.2631578947
0.40526315790.34210526321.42105263160.33684210530.2789473684
0.46315789470.37894736841.49473684210.32631578950.3
a-L-AF 70
a-L-AF 60
Xyl 10 A
a-Gal A
Pec
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
00000
0.14693877550.15510204081.57473684211.65894736841.6421052632
0.27755102040.28571428573.38526315793.18315789473.4526315789
0.310.44081632657.55789473686.42105263167.4736842105
0.27346938780.416326530611.62105263168.378947368411.7052631579
0.25578231290.576870748312.463157894710.273684210513.4736842105
substrate
b-glu A. j.
Spezyme 0,1 g/l
Multifect 0,1 g/l
S 0,07 g/l + M 0,025 g/l
t, h
CRS, g/l
Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.
2 h6 h2448729602624487296
C. lucknowenseC. lucknowense
1CBH Ia 65 kDaCBH Ia 65 kDa
2CBH Ia 65 kDa0.50.260.650.50.370.43CBH Ia 65 kDa0.100.000.000.02.870.0
3CBH Ib 60 kDa0.450.210.450.380.30.38CBH Ib 60 kDa0.090.000.000.02.260.0
4CBH IIb 70 kDa0.570.310.640.410.280.33CBH IIb 70 kDa0.110.000.000.02.090.0
5EG II 51 kDa0.640.360.750.490.340.4EG II 51 kDa0.130.000.000.02.610.0
6EG V 25 kDa0.510.280.440.270.360.25EG V 25 kDa0.100.000.000.01.390.0
7EG VI 47 kDa0.470.250.500.330.430.29EG VI 47 kDa0.090.000.000.01.700.0
8Xyl II 24 kDa0.630.160.270.160.20.42Xyl II 24 kDa0.000.000.000.00.700.8
9b-Glu 106 kDa0.820.230.300.190.230.17b-Glu 106 kDa0.000.000.000.00.830.0
T. reesei0.07T. reesei
10CBH I hm0.560.361.020.650.460.59CBH I hm0.110.000.000.03.650.0
11CBH I lm0.510.240.530.450.370.41CBH I lm0.100.000.000.02.870.0
12CBH II hm0.60.330.590.350.430.28CBH II hm0.120.000.000.01.700.0
13CBH II lm0.670.350.610.40.250.29CBH II lm0.140.000.000.01.830.0
14EG I hm0.540.330.710.440.290.35EG I hm0.110.000.000.02.170.0
15EG I lm0.720.410.730.430.30.35EG I lm0.150.000.000.02.260.0
16EG II hm0.70.340.600.350.220.26EG II hm0.140.000.000.01.570.0
17EG II lm0.590.30.450.240.160.2EG II lm0.120.000.000.01.040.0
18Xyl II0.940.250.420.250.280.2Xyl II0.000.000.000.01.040.0
19substrate0.570.140.150.090.110.15substrate0.000.000.000.00.240.2
20b-glu A. j.0.770.170.230.120.150.23b-glu A. j.0.000.000.000.00.380.4
1441020
0.5
26 h2448729602624487296
C. lucknowenseC. lucknowense
1CBH Ia 65 kDaCBH Ia 65 kDa
2CBH Ia 65 kDa0.420.130.660.80.580.34CBH Ia 65 kDa0.000.000.000.00.00.0
3CBH Ib 60 kDa0.380.120.310.460.50.28CBH Ib 60 kDa0.000.000.000.00.00.0
4CBH IIb 70 kDa0.970.210.440.780.940.18CBH IIb 70 kDa0.000.000.000.00.00.0
5EG II 51 kDa1.60.420.980.470.70.44EG II 51 kDa0.000.000.000.00.00.0
6EG V 25 kDa20.720.781.120.600.34EG V 25 kDa0.000.000.000.00.00.0
7EG VI 47 kDa1.110.20.440.610.25EG VI 47 kDa0.000.000.000.00.00.0
8Xyl II 24 kDa3.923.143.140.440.61.5Xyl II 24 kDa0.000.000.000.00.00.0
9b-Glu 106 kDa0.660.250.280.400.500.07b-Glu 106 kDa0.000.000.000.00.00.0
T. reeseiT. reesei
10CBH I hm0.870.31.220.710.910.71CBH I hm0.000.000.000.00.00.0
11CBH I lm0.430.110.470.280.930.2CBH I lm0.000.000.000.00.00.0
12CBH II hm0.60.10.273.061.30.16CBH II hm0.000.000.000.02.10.0
13CBH II lm0.880.110.360.620.950.18CBH II lm0.000.000.000.00.00.0
14EG I hm1.050.331.183.020.70.45EG I hm0.000.000.000.00.00.0
15EG I lm1.2310.82.480.780.56EG I lm0.000.000.000.00.00.0
16EG II hm1.040.60.71.570.940.2EG II hm0.000.000.000.00.00.0
17EG II lm0.140.110.06511.20.2EG II lm0.000.000.000.00.00.7
18Xyl II3.763.13.281.40.671.11Xyl II0.000.000.000.00.00.0
19substrate0.120.10.0360.430.270.09substrate0.000.000.310.00.30.3
20b-glu A. j.0.140.110.030.780.860.2b-glu A. j.0.000.000.000.00.60.5
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- () (IV) - Penicillium 537 (CBH1 ) -GCBH-s
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(.)The Chemical Journal, .2008, .39
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