Post on 05-Jan-2016
description
Sabato D’AuriaIstituto di Biochimica delle ProteineConsiglio Nazionale delle Ricerche
s.dauria@ibp.cnr.it
Progettazione e Sviluppo di innovativi Nanosensori a Fluorescenza per la Sicurezza e la Difesa Nazionale
Ricerca e Tecnologia per la Sicurezza. Auditorum di Finmeccanica, Roma 10 Novembre, 2005
Biological recognitionBiological recognition elementelement
transducertransducer
amplifieramplifier
microelectronicsmicroelectronics
analytesanalytes
Biological Recognition Biological Recognition ElementElement EnzymeProtein
Antibody
Signal TransducerSignal Transducer Optical
Electro-ChemistryCalorimetric
Roma 10 Novembre, 2005
The molecular complexity of chemical sensors quickly increases for complex biochemical analytes. Even if an appropriate probe could be designed and synthesized, there is no guarantee of useful spectral changes, adequate water solubility, and right affinity constants.
Why Proteins, but not Chemical Probes for sensing analytes???
The sensor showed on the next slide illustrates the complexity of fluorescence chemical probes.
Roma 10 Novembre, 2005
O ON
NN
HN
O
Pr
Pr
PrNH
O
O
O
O
N
H
H
O
O
O
P O
O
N
NN
N
NHH
Pr
Pr
PrN
O
OH
O
NH
OH
Structure of the chemical probe used for fluorescence detection of AMP
Roma 10 Novembre, 2005
The Complexity of a Fluorescence Chemical Probe
Roma 10 Novembre, 2005
Thermostable proteins as long-term stability probes: example of glucose sensing by mesophilic and thermophilic glucokinase
Third millennium biosensors will have the following features:
- Long-term Stability
- Non-Consuming analyte
- Implantable
- Wireless
Roma 10 Novembre, 2005
Selective binding of proteins to irradiated PS regions with submicrometer resolution. (2005 International Patent Pending)
Nanotechnology approach for stable protein-based nanosensors
Roma 10 Novembre, 2005
Roma 10 Novembre, 2005
Advanced Robot Machine for High Density Nano-patterning Sensing Biomolecules on Chips
Roma 10 Novembre, 2005
Thermostable Glucose-Binding Protein from P. horikoshii for glucose sensing
Roma 10 Novembre, 2005
Multi-analyte assay by nano-patterning biomolecules specifically labeled
Roma 10 Novembre, 2005
Fluorescence Platform for advanced sensing methodologies
Family doctor and/orHospital
Wireless communicationCoenzyme-depleted enzymes and proteins belonging to the “binding-protein” family can be used as specific probes for the realization of non-consuming analyte fluorescence biosensors for analyses of high social interest.
Labeled proteins immobilized on the chip
Roma 10 Novembre, 2005
Roma 10 Novembre, 2005
Wireless biosensors for homeland security
Fluorescence emission of a biomolecule immobilized on a porous silicon chip. Fluorescence variations of Acetylcholine immobilized on a Porous silicon chip upon binding ammonium nitrate
Chips at Work
Roma 10 Novembre, 2005
Conclusions
The use of specific biomolecules as fluorescence probes results in the design of advanced highly stable and sensitive nanosensors for analyses of high social interest.
Fundings
- Consiglio Nazionale delle Ricerche - Commesa: Diagnostica Avanzata ed Alimentazione
- Regione Campania Centro di Competenze in Biotecnologie Industriali
- NATO Grant PST CLG 981025
- NATO Grant LST 978934
- Ministero Affari Esteri - Italia- Corea del Sud – Grant N. R1
- Ministero Affari Esteri – Italia - Austria- Grant N. 4-2004-Grant CNR-Kosef
Roma 10 Novembre, 2005
Roma 10 Novembre, 2005
- Selex Sistemi Integrati, Dr. Carlo Falessi (MIUR Project, Submitted 2005)- Harvard University, Prof. J. Collier (Progetto Italy-USA, MIUR, Submitted 2005)- University of Texas, USA, Dr. I. Grycznyski (Core collaboration)- Russian Academy of Science (FIRB Project, with Selex SI, Submitted 2005)- ISS, Inc, Illinois, USA, Dr. B. Barbieri (Core Collaboration)- University of Prague, Dr. P. Herman (NATO)- University of Pussan, Korea, Dr. J. Kang (CNR; Kosef)- Institute Galileo Ferraris, Torino, Dr. A. Rossi (Core Collaboration)- Institute of Microelectronics, Dr. L. DeStefano (Core Collaboration)- University of Graz, Austria, Dr. B. Nidetzky (International Project)- Neotron, Spa, Modena, Italy, Dr. M. Gatti (Collaboration Project)
Collaborations
The 2004-2005 Lab’s Publications on Design and Development of Advanced Sensing Methodologies
- Odor-binding protein as probe for a refractive index-based biosensor: new perspectives in biohazard assessment. S. D’Auria, A. Varriale, M. Staiano, V. Scognamiglio, M. Rossi, S. Campopiano, V. Cennamo, L. Zeni ,(2005) Proteins Peptide Letters (in press)- Advanced Protein-Based Biosensors: Glucose Biosensors as a Model for Analyses of High Social InterestM. Staiano, P. Bazzicalupo, M. Rossi, and S. D’AuriaMolecular BioSystems (2005) DOI:10.1039- Porous silicon-based optical microsensor for the detection of L-Glutamine L. De Stefano, L. Rotiroti, I. Rendina, L.Moretti, V. Scognamiglio, M. Rossi, and S. D’Auria(2005) Biosensors Bioelectronics (in press)- Writing 3D protein nanopatterns onto a silicon nanospongeS. Borini, S. D’Auria, M. Rossi, A. M. Rossi(2005) Lab-on-a-Chip Oct;5(10):1048-52- Advanced Fluorescence Biosensors for Diabetic patientsS. D’Auria, V. Scognamiglio, M. De Champdore’, M. Staiano, G. Ghirlanda, M. Rossi(2005) Topics in Fluorescence Spectroscopy,10, Plenum Press, New York, USA- A putative thermostable sugar-binding protein from the archaeon Pyrococcus horikoshii as a probe for the development of a fluorescence biosensor for diabetic patients.M. Staiano, MR Sapio, V. Scognamiglio, A. Marabotti, AM Facchiano, P. Bazzicalupo, M. Rossi, and S. D’Auria Biotechnology Progress (2004) 5:1572-80.- Protein-based biosensors for diabetic patientsV. Scognamiglio, M. Staiano, M. Rossi, and S. D’Auria.Journal of Fluorescence (2004) 14,5,491-498.
Roma 10 Novembre, 2005
Personnel
Prof. Mose’ Rossi, DirectorDr. Vincenzo Aurilia, Molecular GeneticistDr. Maria Staiano, BiochemistDr. Marcella de Champdore’, ChemistDr. Viviana Scognamiglio, BiologistDr. Antonio Varriale, BiotechnologistDr. Antonietta Parracino, ChemistMr. Gianluca Aquino, StudentMrs. Annalisa Vitale, StudentMrs. Immacolata Cocozza, Student
Roma 10 Novembre, 2005