CRYPTOCOCCUS NEOFORMANSCRYPTOCOCCUS NEOFORMANSPresentation subtitlePresentation subtitle

SEJARAH• Sanfelice 1894 : peach juice Italy (Sanfelice, 1894 )• Busse dan Buschke ,1895 : cryptococcosis : chronic ulcer tibia• Verse 1914 : cryptococcal meningitis• Stoddart dan Cutler 1916 : patologi CNS → kapsul polisakarida

Struktur sel Cryptococcus Neoformans (Sumber : Simmer dan Secko, 2009)

MIKROSKOPIS

(Perfect, 2010).

SIKLUS HIDUP

. Diferensiasi morfologis pada CN (Sumber : Lin, 2009)

Komplek spesies CN Cryptococcus Neoformans

Serotipe Representasi klinik

Filamentasi Perkawinan

Filamentasi sendiri

Pseudohifa

Cryptococcus Neoformans

A var. grubii 95% Ya Tidak Ya AD hybrid

D var. neoformans

<5% Ya Ya Ya

Cryptococcus Gattii

B <1% Ya Tidak Tidak C

• C. gattii (serotypes B and C) is most often isolated from trees

• C. neoformans (both serotypes A, D, and AD hybrids) is more often isolated from bird guano

• Basidiospora/ ragi kering inhalasi paru granuloma (bentuk dormant) imunosupresi menyebar

CRYPTOCOCCUS GRUBII

From left to right:•Encapsulated yeast cells•Sexual spores from Serotype A mating•Encapsulated yeast and sexual spores on defined media•Basidia head bearing four spores at distinct positions•Hyphae with a clamp cell, yeast cells and sexual spore chains(Source: Rajesh Velagapudi, and Joseph Heitman, M.D, PhD., Duke University)

C VAR NEOFORMANS

Velagapudi et al., 2009

C GATTII

Velagapudi et al., 2009

Perbandingan

Velagapudi et al., 2009

Perbandingan

Comparison of morphologies and spore sizes. Spores produced by mating, fruiting, or diploid self-filamentous C. neoformans strains were analyzed by SEM. A comparative profiling of the spores from H99 and KN99a serotype A mating (a), JEC21 and JEC20a serotype D mating (b), and monokaryotic fruiting of serotype D strain XL280 (c) and spores produced by self-filamentous diploid (aAA) strain KN2B5#19 (d) is shown.

Velagapudi et al., 2009