Elias Hakalehto, PhD

Adjunct Professor in University of Helsinki and

University of Eastern Finland, Finland

CEO, Finnoflag Oy

Elias Hakalehto 6.6.17 Villa Solin, Turku

Biojalostus, ekosysteemipalvelut ja

mikrobiomit kestävän kierrättämisen

ytimessä – kasvavaa liiketoimintaa

luonnon ehdoilla

"Itämeren puhtaimmat liiketoimintamallit",

Villa Solin, City of Turku, Finland 6.6.2017

, Finland

Hakalehto, E. 2015. Enhanced microbial process in the sustainable fuel production. In Jinyue, Y

(Ed.). Handbook of Clean Energy Systems. J.R.Wiley & Sons USA.

Elias Hakalehto 6.6.17 Villa Solin, Turku

Elias Hakalehto Tampere 5.6.17

What is "disruptive technology"?

Which technologies are indeed irreplaceable...?

British Airways Magazine 2017

Elias Hakalehto 6.6.17 Villa Solin, Turku

... for the global survival?

Solar panels?

Fuel cells?

Spacecrafts?

Lithium batteries?

Robot cars?

5 G ?

Microbial biotechnology?

Elias Hakalehto 6.6.17 Villa Solin, Turku

SIDE STREAM

PRODUCTIZATION

BIOCHEMICALS,

SUSTAINABLE FOOD-CHAIN

AND ENERGY

ZERO WASTE

Elias Hakalehto 6.6.17 Villa Solin, Turku

Microbes fascinate Bill Gates. On his arrival for a recent meeting with Justin

Trudeau, the Canadian Prime Minister, Gates is spotted clutching a book on

bacteria, presumably so that he might devour a chapter or two should any lull occur

in the conversation.

When we meet shortly afterwards, Gates – though minus scientific tome – has the

faintly restless manner of someone who finds micro- organisms more absorbing

than some dignitaries and most journalists. Gates and I have met several times, and

I think I know his approach. Though unfailingly modest and courteous, he loathes

wasting a single second.

When it comes to changing the world, however, no topic is too small to engage his

attention, and none too vast. He is, for instance, so intrigued by chickens and

their potential to feed the hungry that he recently addressed a conference

accompanied by a coop full of the birds.

Mary Riddell, The Daily Telegraph, 21st October, 2016

USES OF CHICKEN

Biogas, compost

Hakalehto, E. (ed.) 2015. Microbiological Food Hygiene.

Nova Science Publishers, Inc., N.Y., USA.

Elias Hakalehto 6.6.17 Villa Solin, Turku

irrigation

agricultural

land

town market

(pediatric)

patients

wastewater pool

Hakalehto, E. 2015. Hazards and prevention of food spoilage. In: Hakalehto, E. (ed.) Microbial food

hygiene. New York, NY, USA: Nova Science Publishers, Inc.

Hakalehto, E., Nyholm, O., Bonkoungou, I.J.O., Kagambega, A., Rissanen, K., Heitto, A., Barro, N. &

Haukka, K. 2014. Development of microbiological field methodology for water and food-chain hygiene

analysis of Campylobacter spp. and Yersinia spp. in Burkina Faso, West Africa. Pathophysiology, 21: 219-

229.

Campylobacter sp.

Yersinia sp.

animal feed

grains chicken

Elias Hakalehto 6.6.17 Villa Solin, Turku

BIOPROCESS

- UTILIZES BIOCATALYSIS

IN BIOREFINERY INDUSTRY

MICROBES

ENZYMES

Elias Hakalehto 6.6.17 Villa Solin, Turku

Photos from Hagbyn organic chicken farm in

Enköping. Below wastes from slaugtered

chicken and manure, which were used as raw

materials. European Union Baltic Sea Project

ABOWE Test Report 2014-2015.

Peeling of apples for extra carbohydrate

and trace element source.

Elias Hakalehto 6.6.17 Villa Solin, Turku

Biogas yield of the different substrates used in the biorefinery pilot runs in Sweden

and the residues/digestate after the first pilot run. The y-axis shows the yield in liter

biogas per grams of volatile solids (ABOWE Swedish Test Report 2014-2015).

BIOGAS PRODUCTION IN SWEDISH TESTS

Elias Hakalehto 6.6.17 Villa Solin, Turku

FATE OF CARBON IN INDUSTRIAL SYSTEMS

WASTE WATER

SLUDGE

WASTE PAPER CELLULOSE PAPER COMBUSTION

WOOD WASTE

CO2, CO, H2S, NOx

PAST IN PAPER AND PULP PRODUCTION

Hakalehto E. International Conference of Environmental Indicators. Windsor, Canada, 3.-5.8.2015

Elias Hakalehto 6.6.17 Villa Solin, Turku

FATE OF CARBON IN INDUSTRIAL SYSTEMS

WASTE WATER

SLUDGE

WASTE PAPER

CELLULOSE PAPER COMBUSTION

WOOD WASTE CO2, ym

FUTURE IN FOREST BIOREFINERIES

SIDE STREAM CHEMICALS

ENERGY, FUEL, BIOCHEMICALS H2 CH4

ORGANIC FERTILIZERS

BIOPROCESSES

TO ASSIMILATION

RECYCLED WATER

Hakalehto E. International Conference of Environmental Indicators. Windsor, Canada, 3.-5.8.2015 Elias Hakalehto 6.6.17 Villa Solin, Turku

X

Elias Hakalehto 6.6.17 Villa Solin, Turku

MICROBES

MUNICIPAL CIRCULATION GARDENS

SLUDGE

NUTRIENTS

PLANTS

ENERGY

GREEN FOOD

ANIMAL HUSBANDRY

DEVELOPMENT OF FOOD SUPPLY

• Recycling biowaste it is possible to get both energy and organic fertilizers. With the help

of these it is possible to cultivate food in urban environmentt with the idea of circular

economy (fig up:www.ryerson.ca (Carrot City, Romainville, Paris))

Chinese cabbage fertilized with patented microbe containing nutrients

(Finnoflag Oy).

Elias Hakalehto 6.6.17 Villa Solin, Turku

IN FUTURE BIOREFINERIES ALL RESIDUAL BIOMASS IS

RETURNED TO THE CIRCULATION

Adj. Prof. Elias Hakalehto 2017

Elias Hakalehto 6.6.17 Villa Solin, Turku

MICROBES FOR GOOD

GREEN FUEL &

ENERGY

WASTE

RECIRCU

LATION

HEALTH

PROMOTION BULK,

PLATFORM &

SPECIAL

CHEMICALS

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

A. UNDERSTANDING MICROBIAL METABOLISM

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

B. INTERACTION BETWEEN NATURAL STRAINS

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

C. LAB -> PILOT -> SCALE UP

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

D. SAVE IN THE INVESTMENT

- NON-ASEPTIC

- FAST THROUHGPUT -> HIGH PRODUCTIVITY

- LOW ENERGY ROUTE

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

E. PRODUCT RECOVERY

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

F. LEARNING SYSTEMS

- CONSOLIDATED BIOPROCESSING

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

G. BIOBASED FINAL PRODUCTS

Aromatics (Phe,

Tyr, His,..)

Glycoside CH’s

Lactate

O-CH’s (sugars)

CH2’s

Acetate

CH3’s

Reference

(TSP)

Hydrophobic

amino acids

WATER

Laatikainen, R., Laatikainen, P., Hakalehto, E. Quantitative quantum mechanical nmr analysis: The superior tool

for analysis of biofluids. In Proceedings of the 1st Int. Electron. Conf. Metabolomics, 1–30 November 2016;

Sciforum Electronic Conference Series, Vol. 1, 2016 , C005; doi:10.3390/iecm-1-C005

Elias Hakalehto 6.6.17 Villa Solin, Turku

KEYS TO SUCCESS IN MICROBIAL BIOPROCESS

H. CLEAN VALUES:

- AIR

- WATER

- SOIL

Hakalehto, E. 2013. Interactions of Klebsiella sp. With other intestinal flora. In Pereira, L.A. &

Santos, A. (eds) Klebsiella infections: Epidemiology, pathogenesis and clinical outcomes. Nova

Science Publishers, Inc. New York, USA.

EXHAUST GASES OF ONE MICROBIAL CULTURE

EXPLOITED BY THE SUBSEQUENT ONES

Elias Hakalehto 6.6.17 Villa Solin, Turku

PMEU Spectrion ®

PMEU with Automated Sample

Collection System

PMEU Scentrion ®

Syringes in the PMEU

(Portable Microbe Enrichment

Unit)

Elias Hakalehto 6.6.17 Villa Solin, Turku

Elias Hakalehto 6.6.17 Villa Solin, Turku

WE HAVE BEEN INVOLVED SEVERAL

BIOREFINERY PILOT PROJECTS SINCE 2007. NOW

IT IS THE TIME FOR UPSCALING…

Elias Hakalehto 6.6.17 Villa Solin, Turku

THANK YOU!

Elias.Hakalehto@gmail.com

Elias Hakalehto 6.6.17 Villa Solin, Turku