Post on 01-Jun-2020
KYOTO UNIVERSITY京都大学
Bio-Sensing Engineering
Naoshi Kondo
Laboratory of Bio-Sensing Engineering
Graduate School of Agriculture
Kyoto University, Japan
Fluorescence Phenomenon on Agricultural Products
KYOTO UNIVERSITY京都大学
来週までのレポート
農産物の蛍光物質についてどのようなことに利用できるか(品質評価,鮮度評価,異物検査,腐敗防止,光合成の活性度,化学薬品の検査),学んだことを例を挙げてレポートしなさい
Spectroscopy
Various type of light-matter interaction
Why Fluorescence and what we can detect with Fluorescence Spectroscopy?
• In general fluorescence emitted by compounds with benzene ring or conjugationsystem in their molecular structure
• Many bio-products are containing fluorophore• Fluorophore could be used as a marker for bio-products content, time series
changes, and physical characteristics.• Interaction of fluorescence phenomenon with other light-matter phenomenon
Beta carotene
CoumarinChlorophyll
KYOTO UNIVERSITY京都大学
Image PC
HostPC
180°Turn over
Image PC
TV Camera Lighting Device
X-ray generator
Scintilator
Imaging PC
JudgementPC
Trigger sensor
Spectrometer
Halogen lamp
Typical Fruit grading system
KYOTO UNIVERSITY京都大学
A Color TV camera-Double LED system
White LED
device
Camera
UV LED device
PC
White LED
device
White LED
device
UV LED device
UV LED device
KYOTO UNIVERSITY京都大学
Rotten Fruit
Slight injury, but possibility to be rotten
White Light UV Light
Fluorescence
Fluorescence Image
KYOTO UNIVERSITY京都大学
Pin hole Rot Grow rotten area Mold Influence
Other fruits
Slight injury makes all fruits in a box spoil
2 weeks1 week
Separate slight injury fruits from healthy fruits
Before storage
KYOTO UNIVERSITY京都大学
Top view of the image acquisition system on an
practical grading line (Shibuya Seiki)
Separated rotting fruits in several hours
Shibuya Seiki
KYOTO UNIVERSITY京都大学
Autonomous controlled drone
Dark part
Scute (Placoid scale, Shield shape scale)
Horse Mackerel (fish name)
KYOTO UNIVERSITY京都大学
Ishida
Parasite worm (Anisakis) detection
3.2 t/h
Applicable to Grain Sorter
ColorCamera
Grain Guide
Background
Color/NIRCamera
Light (CCFL)
NIR LED
Bad grain Good grain
EjectorNozzle
Possibility to
separate by FL
Satake
KYOTO UNIVERSITY京都大学
Activities of Fluorescence Substances
Excitation
280 nm
230 nm
KYOTO UNIVERSITY京都大学
Flavonoid change
0
1
2
3
4
4 6 8 10 12 14 16 18 20Inte
nsity R
atio
Ex 2
80
/Ex 2
30
Day After Harvest
Intensity Ratio Flavonoids Exocarp
Ex 280/Ex 230
Stored in 4 C
Stored in 20 C
(Yang et al.,2011, total flavonoid in green peppers increased during storage)
Freshness index?
KYOTO UNIVERSITY京都大学
Photosynthesis activities in Periwinkle (plant name)
FL. Intensity of shady
leaves is higher
Hidden part of leaf is higher
KYOTO UNIVERSITY京都大学
Family of cucumber plant
Disease white dots are
highlighted Yellowish weaken
leaves are low intensity
Greenish healthy leaves
are high intensity
Induction method to assess the
photosynthetic function in tomato plant
Time (s)
Ch
l flu
ore
sce
nce
inte
nsity
(r.u
.)
1 10 100
Light on
I D
P
S
M
T
Excitation light intensity
P/S
M/S
Chl fluorescence induction
Courtesy of Prof. Takayama, Ehime Univ.
1.5
m
0.6 m
CFI robot for practical use
0.6 m
CCD camera120°2.1m
Tomato plant
PC
CCD
camera
Automatic
operation cart
Blue LED for
excitation light
Chl fluorescence
image
Iseki Co., Ltd.: by courtesy of Prof. Takayama, Ehime University
Applicable to seedling production robots
Grafting robot
Cutting sticking robot
Transplanting robot
BRAIN
KYOTO UNIVERSITY京都大学
Collaboration research: Tea leaves fluorescence Kyoto Prefectural Tea Industry Research Institute
KYOTO UNIVERSITY京都大学
Chemical detection
Possibility of
chemical residues
detection
Rotting
Actively add fluorescence substance to spreading agent
Fertilizers
Chemical Fertilizer
N-P-K=14-10-13
Chemical Fertilizer
N-P-K=14-2-16
Lime
Organic fertilizer
(Cattle manure) Greenish
Blueish
Reddish
Measurement Box
Power source, PC,
Spectroscopy,
PLC, amp board
Antenna of
DGPS
Chisel
(sensor
probe
housing)
※ Date: 2004. 11. 21 Depth: 150mm, Speed: About 30cm/ sec
Cooperation with SHIBUYA MACHINERY CO.,LTD., TUAT
Commercialized Real time soil sensor
Applicable to soil sensor
Shibuya
KYOTO UNIVERSITY京都大学
Arrangement of Sensors in chisel
Illumination fiber
EC Electrode
Laser Displacement
sensor Color cameraCondensed
fiberGround surface
Soil flattener
Courtesy of Prof. Shibusawa, TUAT
KYOTO UNIVERSITY京都大学
Effect of fertilizer treatment on carrot fluorescence
Chemical fertilizer Organic fertilizer
KYOTO UNIVERSITY京都大学
Effect of fertilizer treatment on carrot fluorescence
Chemical fertilizer Organic fertilizer
Moisture
Content
NO3-NSOM
pH EC
Real-time soil sensor
N, EC, pH, moisture content, SOM,
soil temperature, compaction
Maps from Soil Sensor
By courtesy of
Prof. Shibusawa, TUAT
Tomato Freshness Monitoring Day 1 3 7 9 11 15
Color
image
UV
image
0
5
10
15
0 5 10 15 20
PW
L
Day
Spoilage limitFRESH
SPOILED
UV image color channel Physiological Weight Loss during storage
0
50
100
150
200
0 5 10 15
HS
V
Day
hue saturation value
Nurulhuda et al. (2017)
Fish eye fluorescence
Day 0
Day 0
Day 3
Day 3
Day 6
Day 6
Peak A
Peak C
Peak B
KYOTO UNIVERSITY京都大学
Collaboration research: Olive oil authentication and quality evaluation
Color image (halogen lamp) and FL image (Ex: 365 nm)A: Coratina; B: Chieti; C: Virgin OO; D: Taggiasca; E: Bosana; F: Unknown, unfiltered;
G: Monte Etna; H: Ogliarola; I: Mix, Coratina, Ogliarola, Signora
Collaboration with University of Molise, Italy
KYOTO UNIVERSITY京都大学
Kyoto University Project “SPIRITS” Construction of Database of Fluorescence Properties in Asian Agriculture,
Livestock, and Aquaculture for 9 Billion People’s Food Production
2. Quality measurement
1. Reduction of the post-harvest loss
Accumulation to DBTemperate Zone
Tropical Zone
(Supporting Program for Interaction-based Initiative Team Studies)
KYOTO UNIVERSITY京都大学
Kyoto University Project on Fluorescence Construction of Database of Fluorescence Properties in Asian Agriculture,
Livestock, and Aquaculture for 9 Billion People’s Food Production
Temperate Zone
Tropical Zone
2. Quality measurement
3. Food safety
4. Plant production control
5. Chemical tracking
1. Reduction of the post-harvest loss
Accumulation to DB
Kyoto Univ.
University
Res. Institute
Res. Institute
University
Company
Res. Institute
Company
Company
Res. InstituteProducts in AgricultureLivestockAquaculture
Properties of Fluorescence
the World Food
Production
Contribution to
DBJoin the members to accumulate Fluorescence Data
20/42
KYOTO UNIVERSITY京都大学
Roles of Grading Facility and Sensors
1. Efficient sorting, and labor saving
2. Uniformization of fruit quality
3. Enhancing market value of the products
(Establishing local region brand of products)
4. Fair payment to producers based
not only on quantity but on quality of each fruit
5. Farming guidance from grading results and GIS
6. Contribution to the traceability system
for food safety and security
7. Saving the food loss
Producer Operator Distributor Consumer