分 子 病 理 實 驗 室Molecular Pathology Lab

實驗室主持人：戚謹文 , 周德盈共同主持人 : 李芬瑤

The development of rational approaches to the diagnosis and treatment of cancer is dependent on the understanding of the mechanism that underlie the formation and progression of tumor

Traditional pathology

Molecular pathology

•Traditional pathology1. Frozen section2. Wax embedded tissue

Hematoxylin-eosin stainSpecial stain: silver stain immunostain: vessel, estrogen receptors

•Advances in molecular biologyGenetic analysesComparative genomic hybridization(CGH) Fluorescence in situ hybridization (FISH)Gene expression: transcriptionGenomic fingerprintingcDNA and oligonucleotide arraysSerial analysis of gene expressionDifferential display or representational difference analysisGene expression: translation2-D gels, HPLC, Mass spectrometry, tissue array, microdissection and laser microscopy

•Molecular pathology (1) Human genome project

1. novel genes 2. known genes : amplification,

mutation, deletion

•Molecular pathology (2) Human genome project

Studies of mRNA can determine the expression profile of a large number of transcripts.

•Molecular pathology (3) Human genome project

Proteomic methodologies can identify quantitative and qualitative protein changes in tumors, and to examine posttranslational modifications such as phosphorylation of proteins.

Loss of annexin 1 correlates with early onset of tumorigenesis in esophageal and prostate carcinoma. Paweletz et al. Cancer Res 60:6293, 2000

•References:

1. Going and Gusterson Molecular pathology and future development. Eur J Cancer, 35:1895-1904, 1

999.2. Sirivatanauksorn et al. Laser-assisted microdissection: applications in molecular biology. J Patholo

gy 189: 150-154, 1999.