Post on 23-Feb-2016
description
PowerPoint Presentation
1 /
12
()
23
()
2011
34
()
ITS-DNAHPLCsyringaldehydeJournal of Nature Products
HPLCsyringaldehydecaffeic acidchlorogenic acidquercetinGlut4 2013
45
- 56
67100-1.2.3--aB101-1.2.2--a7
78 OGTT
89
9 10
10 2013()11
-11 2013()12
--12 2013 13
13 2013 14
20131415 (1)(2) (3)
15 ()syringaldehydecaffeic acidchlorogenic acidquercetin
(lignans) (Phenylpropanoids(polyphenol) (triterpenoids)
161617lignin triterpenoid, phenylpropanoid estersphenolicMyriceric acid Hibicusin(7S,8S)-Demethylcarolignan Eerythro-Carolignan EBoehmenanMansonone H Myriceric acid B/CUncarinic acid A (A549MCF-7 )Syringaresinol (human breast cancer cellsP- Glycoprotein )(P -)cleomiscosin-A (antileukemia )syringic acid, Vanillic Acid ()scoparoned () 9,9-O-Ferulolyl-()-secisolaricinresinol, uncarinic acid (HIV)() phenylpropanoids dihydrodehydrodiconifenyl alcohol ( NGF) ()17Journal of Natural Products 18
2012 Journal of Natural Products syringaldehyde 1819
19 20
1H-NMR
Mass20 ()21
21 22
22 23
23
24
A54924A549 25
A549A5491224A549Myriceric acid(7S,8S)-Demethylcarolignan EHibicusinHibiscone CHibicuslide Cerythro-Carolignan EBoehmenanMansonone HMyriceric acid B/CA549
25
A549 cell
26Flow cytometry12hrA549cell cycle S phaseA54915.625 ug/ml (3%)250 ug/ml (18.5%)
26()27
2728Hibiscus Taiwanese()A549BCCA54962.5g/ml27%125g/ml82%
BCC31.25g/ml25%62.5g/ml70%125 g/ml90%
2828 29
2930Anti-Inflammatory and Analgesic Effects
Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 132859, 13 pages doi:10.1155/2012/1328593031
3132
A(PGE2) 150300g/mlPGE2
B() 2
3233COX-2PGiNOSCOX-2COX-2
3334
HIV
188020051153551HIV(8) 3435
Bioorganic & Medicinal Chemistry
200412 Bioorganic & Medicinal ChemistryEC 50 values of 1.8 and 3.9g/mL (7) 3536 (I~III)
(Hibiscus taiwanensis)(Malvaceae)
lignans 1532 naphthoquinones 1016 40naphthalene 5 oleanoic acids 282930 31
hydroxyl groupmethoxyl group cinnamic acid or ester110(6) 3637 1 2
Met3738
138
393940
40 ()
414142 ()
...
4243
4344 -
109(90/) 3
14323
1 2 3
4445 ()1 Ans
2Ans
3Ans
4546()4 Ans
5Ans
6Ans 1-3
4647
4748
-
48Chart11000096.82295.51172.43317.31112.32225.144
Hibiscus Taiwanese (ug/ml)Cell ViabilityvA549
Sheet1100096.8295.5172.4317.3112.3225.14Control15.62531.2562.5125250500
Sheet1000022011033011022044
Hibiscus Taiwanese (ug/ml)Cell ViabilityA549
Sheet2
Sheet3
Chart110000912276113144113310441355
Hibiscus Taiwanese (ug/ml)Cell V iab ilityBCC
Sheet11000912761314113104135Control15.62531.2562.5125250500
Sheet1000022011044033044055
Hibiscus Taiwanese (ug/ml)Cell ViabilityBCC (Basal Cell Carcinoma)
Sheet2
Sheet3